Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

Your Selections

Mammalian Expression

76,413 results

Showing: 2241 - 2280 of 76413 results

  3. pcDNA3-TfR-OVA

    Plasmid
    #64600
    Purpose
    Expression of chicken ovalbumin fused to the transmembrane domain of transferrin receptor as a model antigen for study of MHC II presentation
    Depositor
    Martin Zenke, Sandra Diebold
    Article
    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.
    Insert
    Ovalbumin
    Use
    Tags
    Transmembrane domain of Transferrin Receptor (TfR…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64600-plasmid-map-sequence-id-117969

    Enlarge

  6. CTGF-bio-His

    Plasmid
    #52028
    Purpose
    Expresses full-length Connective tissue growth factor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    CTGF (CCN2 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52028-plasmid-map-sequence-id-80102

    Enlarge

  8. SpCas9-NG-ABE8e(V106W)

    Plasmid
    #198533
    Purpose
    Expresses human codon-optimized SpCas9-NG-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8e(V106W)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-ABE8e(V106W)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198533-plasmid-map-sequence-id-388880

    Enlarge

  9. EF1a_Puro_Telo_v2

    Plasmid
    #195139
    Purpose
    ~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction
    Depositor
    Jason Sheltzer
    Article
    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11
    Insert
    Telomere (RTEL1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    195139-plasmid-map-sequence-id-442720

    Enlarge

  15. pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt

    Plasmid
    #175181
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Has Service
    AAV1
    Insert
    pAAV hSyn FLEX iGluSnFR3 v857.GPI
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    GPI Anchor, IgK-chain, and Myc epi tag
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    175181-plasmid-map-sequence-id-345963

    Enlarge

  19. TFORF1217

    Plasmid
    #142757
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PRRX1 (PRRX1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    142757-plasmid-map-sequence-id-391466

    Enlarge

  23. pLV-EF1A-CD19.28H.28TM.BB.3z

    Plasmid
    #200679
    Purpose
    Modular CD28hinge-CD28transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production
    Depositor
    Michele Bernasconi
    Article
    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):
    Insert
    FMC63-CD28HD-CD28TM-41BB-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)
    Use
    Lentiviral
    Tags
    MycTag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a-short
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    200679-plasmid-map-sequence-id-392606

    Enlarge

  25. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

    Plasmid
    #62723
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Has Service
    AAV1 and AAV5
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    human synapsin promoter
    Available since
    April 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62723-plasmid-map-sequence-id-166279

    Enlarge

  26. pLenti puro HA-Ubiquitin

    Plasmid
    #74218
    Purpose
    Lentiviral expression of HA tagged Ubiquitin
    Depositor
    Melina Fan
    Insert
    ubiquitin (UBB Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Single ubiquitin coding sequence (full length UBB…
    Promoter
    CMV
    Available since
    March 14, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    74218-plasmid-map-sequence-id-148715

    Enlarge

  32. mRFP-Rab7

    Plasmid
    #14436
    Purpose
    Mammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.
    Depositor
    Ari Helenius
    Article
    Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.
    Insert
    Rab 7 (RAB7A Canis familialis)
    Use
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    3 silent mutations near the C-terminus
    Promoter
    Available since
    March 23, 2007
    Availability
    Academic Institutions and Nonprofits only
    14436-plasmid-map-sequence-id-229535

    Enlarge

  33. TFORF1167

    Plasmid
    #142744
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    EBF3 (EBF3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142744-plasmid-map-sequence-id-395819

    Enlarge

  38. CROPseq-3P5P

    Plasmid
    #219680
    Purpose
    CROPseq vector enabling gRNA detection via mRNA-based capture (CROPseq), 3'-based direct capture using 10x capture sequence 1 or 5'-based enrichment using a custom reverse transcription primer
    Depositor
    Christoph Bock
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    219680-plasmid-map-sequence-id-462884

    Enlarge

Showing: 2241 - 2280 of 76413 results