78,292 results

1. pEGFP-MRLC1

   Plasmid

   #35680

   Depositor

   Tom Egelhoff

   Article

   Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

   Insert

   MRLC1 (MYL9 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pU6-sgRNA EF1Alpha-puro-T2A-BFP

   Plasmid

   #60955

   Purpose

   Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

   Depositor

   Jonathan Weissman

   Article

   Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-PE2max-BSD

   Plasmid

   #191102

   Purpose

   Lentiviral expression plasmid of PEmax prime editor with P2A-BSD marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   PEmax-P2A-BSD
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Changed R221K, N394K, H840A from SpCas9

   Promoter

   EF-1a

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX458-mcherry

   Plasmid

   #161974

   Purpose

   Expresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNA

   Depositor

   Joanna Wysocka

   Article

   Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

   Insert

   Cas9-t2a-mcherry
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_53BP1_FLAG

   Plasmid

   #237345

   Purpose

   transient overexpression in mammalian cells

   Depositor

   Michele Pagano

   Insert

   53BP1 (TP53BP1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV R-CEPIA1er

   Plasmid

   #58216

   Purpose

   Red fluorescent indicator for calcium signaling in the endoplasmic reticulum

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   R-CEPIA1er
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   R-GECO1 E300D M305L D329N F332I E336D N346K F361W…

   Promoter

   CMV

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGP-CMV-jGCaMP8m

   Plasmid

   #162372

   Purpose

   Mammalian expression of ultrafast protein calcium sensor

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8m
   

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   A25G F286Y

   Promoter

   CMV

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-PCNA-19-SV40NLS-4

   Plasmid

   #55117

   Purpose

   Localization: Nucleus, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

9. PCMV4-3 HA/IkB-alpha(SS32,36AA)

   Plasmid

   #24143

   Depositor

   Warner Greene

   Article

   Sun et al Mol Cell Biol. 1996 Mar . 16(3):1058-65.

   Insert

   IkB alpha (NFKBIA Human)
   

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   S32A S36A

   Available Since

   March 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. plenti-CAG-IRF4-FLAG-IRES-GFP

    Plasmid

    #107389

    Purpose

    Lentiviral expression of IRF4-FLAG

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IRF4 (IRF4 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-EF1a-TetOn3G

    Plasmid

    #104543

    Purpose

    PiggyBac vector encoding Tet-On 3G constitutively expressed by EF1a promoter

    Depositor

    David Vereide

    Article

    Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

    Insert

    Tet-On 3G
    

    Expression

    Bacterial and Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. GPI-mCherry2

    Plasmid

    #182869

    Purpose

    Monomer expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

    Insert

    GPI-mCherry2
    

    Expression

    Mammalian

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Flag-SIRT1

    Plasmid

    #1791

    Purpose

    Mammalian expression of flag-tagged SIRT1

    Depositor

    Michael Greenberg

    Article

    Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

    Insert

    SIRT1 (SIRT1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCV_IRES_GFP_CD16a

    Plasmid

    #196189

    Purpose

    Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

    Depositor

    Ana Ruiz-Saenz

    Article

    Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

    Insert

    CD16a (FCGR3A Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    changed Phenylalanine 158 to Valine

    Promoter

    LTR

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-C1-PKM2

    Plasmid

    #64698

    Purpose

    Expression of GFP tagged PKM2 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Stetak et al Cancer Res. 2007 Feb 15;67(4):1602-8.

    Insert

    PKM2 (PKM Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBITE-CD19-CD3

    Plasmid

    #190677

    Purpose

    Encodes a modularized form of HD37-OKT3 bispecific antibody

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Modularized-HD37-modularized-OKT3-P2A-NeonGreen
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. HRE-oUnaG

    Plasmid

    #201712

    Purpose

    Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a

    Depositor

    Friedemann Kiefer

    Article

    Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

    Insert

    UnaG
    

    Expression

    Mammalian

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-IDO1-p2a-eGFP

    Plasmid

    #187026

    Purpose

    Expresses IDO1 and GFP (seperately) in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Maassen et al Free Radic Biol Med. 2022 Aug 1;188:287-

    Insert

    Indoleamine 2,3-dioxygenase 1 (IDO1 Human)
    

    Tags

    P2A-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFRT-DHX36iso1

    Plasmid

    #159585

    Purpose

    Express N-ter FLAG HA tagged DHX36 isoform 1 in mammalian cells

    Depositor

    Markus Hafner

    Article

    Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

    Insert

    DHX36 (DHX36 Human)
    

    Tags

    FLAG HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-Spike-VLP

    Plasmid

    #173922

    Purpose

    Expresses virus-like particles based upon the original SARS-Cov-2

    Depositor

    Andreas Stuermer

    Inserts

    SARS-2 Spike Protein (S Synthetic, SARS-COV-2, Human)
    SARS-Cov-2 E protein (E Synthetic, SARS-COV-2, Human)
    SARS-Cov-2 M Protein (M Synthetic, SARS-COV-2, Human)
    

    Tags

    no tags, native protein

    Expression

    Bacterial and Mammalian

    Promoter

    22bp IRES, CMV, and IE2 Promoter/Enhancer from Mo…

    Available Since

    April 11, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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