74,918 results

1. MYC-hTRAK2

   Plasmid

   #225142

   Purpose

   Expresses MYC-tagged human TRAK2

   Depositor

   Thomas Schwarz

   Article

   Davis et al Life Sci Alliance. 2022 Oct 27;6(1):e202

   Insert

   TRAK2 (TRAK2 Human)
   

   Use

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR.hU6hH1

   Plasmid

   #32686

   Purpose

   dual shRNA expression cassette entry vector

   Depositor

   Ming-Sound Tsao

   Article

   Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

   Type

   Empty backbone

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6hH1

   Available since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1-TALEN-L

   Plasmid

   #59025

   Purpose

   TALEN designed to target the human AAVS1/PPP1R12C locus

   Depositor

   Danwei Huangfu

   Article

   Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

   Insert

   AAVS1-TALEN-L
   

   Use

   AAV and TALEN

   Tags

   3xFlag and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. TDP-REGv2(mScarlet-FLAG)#9 in pTwist-CMV backbone

   Plasmid

   #216153

   Purpose

   Expresses mScarlet in cells with TDP-43 loss-of-function. Has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells (brighter than #7). It uses a single intron. [Code 'B11']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

   Depositor

   Pietro Fratta

   Article

   Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

   Insert

   mScarlet with cryptic splice site and C-terminal FLAG tag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Pb-TRE3G-SpCas9-IRES-blast

   Plasmid

   #195506

   Purpose

   Inducible Piggybac vector expressing SpCas9 with Blasticidin selection

   Depositor

   Joanna Wysocka

   Article

   Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

   Insert

   spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-MS2-HA-HsNBAS_AA

   Plasmid

   #148236

   Purpose

   Mammalian Expression of HsNBAS

   Depositor

   Elisa Izaurralde

   Insert

   HsNBAS (NBAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVUHshp53

   Plasmid

   #11653

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   GFP, shRNA against TP53 (TP53 Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-TXNIP

   Plasmid

   #18758

   Depositor

   Clark Distelhorst

   Article

   Wang et al Oncogene. 2006 Mar 23. 25(13):1903-13.

   Insert

   thioredoxin-interacting protein (Txnip Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1-TALEN-R

   Plasmid

   #59026

   Purpose

   TALEN designed to target the human AAVS1/PPP1R12C locus

   Depositor

   Danwei Huangfu

   Article

   Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

   Insert

   AAVS1-TALEN-R
   

   Use

   AAV and TALEN

   Tags

   3xFlag and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMyrPalm_mEGFP

    Plasmid

    #21037

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    pMyrPalm
    

    Use

    Tags

    mEPFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA 3.3 LRP5

    Plasmid

    #115907

    Purpose

    Expresses human LRP5 in mammalian cells.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    LRP5 (LRP5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. NpY-mNeon

    Plasmid

    #186618

    Purpose

    Encodes Neuropeptide Y fused to mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

    Insert

    Neuropeptide Y (NPY Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA C12 ecDHFR DD YFP HA

    Plasmid

    #192815

    Purpose

    Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.

    Depositor

    John Hulleman

    Article

    Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021

    Insert

    E. coli dihydrofolate reductase
    

    Use

    Tags

    enhanced yellow fluorescent protein and hemagglut…

    Expression

    Mammalian

    Mutation

    W74R/T113S/E120D/Q146L

    Promoter

    CMV

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-TOM20_Halo_T2A_EGFP

    Plasmid

    #135443

    Purpose

    Mamalian cell expression of mictochondrial HaloTag7 and cytosolic EGFP

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

    Insert

    TOM20-HaloTag7 and EGFP
    

    Use

    Tags

    TOM20

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Flag-Gsdmd-NT 4A

    Plasmid

    #80952

    Purpose

    Expresses Gsdmd-NT 4A mutant in mammalian cells

    Depositor

    Judy Lieberman

    Article

    Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

    Insert

    Gsdmd-NT 4A (Gsdmd Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZac2.1 gfaABC1D-cyto-GCaMP8s

    Plasmid

    #186052

    Purpose

    AAV-mediated expression of GCaMP8s under the control of GfaABC1D promoter

    Depositor

    Loren Looger

    Insert

    jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    GfaABC1D

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Nsp7-mCherry

    Plasmid

    #165134

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp7 (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRluc-LC3G120A

    Plasmid

    #105003

    Purpose

    Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.

    Depositor

    Marja Jããttelã

    Article

    Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

    Insert

    LC3B (Map1lc3b Rat)
    

    Use

    Luciferase

    Tags

    Rluc with C124A substitution

    Expression

    Mammalian

    Mutation

    Glycine 120 mutated to alanine, prevents lipidati…

    Promoter

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. MAC-FURIN

    Plasmid

    #158386

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    furin, paired basic amino acid cleaving enzyme (FURIN Human)
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pHTN-HaloTag_BCL11A:1-773

    Plasmid

    #211014

    Purpose

    Mammalian expression of full-length human BCL11A. N-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    BCL11A:M1-F773 (BCL11A Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    isoform 2

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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