76,419 results
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mito-dendra2
Plasmid#55796Purposephoto-activatable mitochondrial marker for expression in mammalian cellsDepositorInsertmito-Dendra2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Wnt3
Plasmid#35909DepositorInsertWnt3 (WNT3 Human)
UseTagsExpressionMammalianMutationNonePromoterCMVAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-CDK8-WT-IRES-mCherry
Plasmid#68856PurposeExpresses Human CDK8 Wild-TypeDepositorInsertcyclin-dependent kinase 8 (CDK8 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1αAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Puro-PNPT1
Plasmid#223315PurposeLentiviral vector expressing human PNPT1DepositorInsertPolyribonucleotide Nucleotidyltransferase 1 (PNPT1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pK036.TRE-Flpe-WPRE (Supernova)
Plasmid#85007PurposeFor Flpe/FRT-based Supernova, pK036 should be used with pK068 etc.DepositorInsertNLS-FLPe-WPRE
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV-LUC2CP/intron/ARE
Plasmid#62858Purposesplicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.DepositorInsertLuciferase
UseLuciferaseTagsExpressionMammalianMutationinsertion of chimeric intron at nueotide poclsiti…PromoterCMVAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
CRY2olig-PR
Plasmid#218917PurposeExpression of CRY2olig-mCherry fused to a codon-optimised poly-PR sequenceDepositorInsertCRY2olig-mCherry tagged poly-PR
UseTagsExpressionMammalianMutationwild-typePromoterCMVAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
CRY2olig-GR
Plasmid#218919PurposeExpression of CRY2olig-mCherry fused to a codon-optimised poly-GR sequenceDepositorInsertCRY2olig-mCherry tagged poly-GR
UseTagsExpressionMammalianMutationwild-typePromoterCMVAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-Oct4-p2a-Sox2-17-t2a-Klf4-e2a-cMyc
Plasmid#193346PurposetetO-OS*KM (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + super-Sox + Klf4 + cMyc)DepositorUseLentiviralTagsExpressionMammalianMutationSox2-17 is engineered highly cooperative chimeric…PromoterTetO mini CMVAvailable sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
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LeGO-S2
Plasmid#85211PurposeLentiviral vector expressing T-Sapphire fluorescent protein driven by SFFV promoterDepositorInsertT-Sapphire
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
Integrin Beta1-mPA-GFP
Plasmid#186302PurposeExpresses mPA-GFP tagged Integrin beta-1 in mammalian cells on a CMV promoterDepositorInsertIntegrin Beta 1 (ITGB1 Human)
UseTagsmPA-GFPExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRGEN-CMV-CjCas9
Plasmid#89752PurposeExpression of CjCas9 in mammalian cellsDepositorInsertCas9
UseCRISPRTagsSV40-HAExpressionMammalianMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-rtTA
Plasmid#163601PurposeExpresses rtTA in mammalian cellsDepositorInsertrtTA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Gα13*-BERKY3
Plasmid#158218PurposeBRET biosensor for detection of Gα13-GTP in mammalian expression vector pcDNA3.1(+)DepositorInsertLyn11-Nluc-ER/K linker-3xYFP-PRG(RH)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
LeGO-mOrange2
Plasmid#85212PurposeLentiviral vector expressing mOrange2 fluorescent protein driven by SFFV promoterDepositorInsertmOrange2
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
DD-Cas9 with filler sequence and Venus (EDCPV)
Plasmid#90085PurposeThis plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be remove for cloning of desired sgRNADepositorInsertCas9
UseCRISPR and LentiviralTagsDestabilized Domain and FlagExpressionMammalianMutationPromoterEFSAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
AIMTOR T757
Plasmid#140828PurposeAIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cellsDepositorInsertAIMTOR T757 (ULK1 Human)
UseTagsNES Nuclear export signalExpressionMammalianMutationThe insert comprises only a small sequence of hum…PromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3 BRE Luciferase
Plasmid#45126DepositorInsertId1-BRE
UseLuciferase; Bmp/smad transcriptional reporterTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMXs GFP-LC3-RFP
Plasmid#117413PurposeExpresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.DepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseRetroviralTagsEGFP and mRFP1ExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-eGFP-Z1
Plasmid#135049PurposeEnhanced Green fluorescent protein expression for mammalian cells under constitutive CMV Promoter in minimal backbone plasmid.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV PromoterAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV INS2x mRFP-3MREs (AAV-beta)
Plasmid#206928PurposeBeta cell-specific gene expression from AAV in human pancreatic isletsDepositorInsertmRFP
UseAAVTagsExpressionMammalianMutationPromoterINSx2Available sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-A315T
Plasmid#133755PurposeLentiviral expression of human TDP-43 A315T disease mutant in mammalian cells.DepositorInsertTARDBP (TARDBP Human)
UseLentiviralTagsExpressionMammalianMutationChanged alanine 315 to threoninePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-EEA-5guides-PGK-Puro
Plasmid#102898PurposeEBNA episome plasmid for U6 promoter-driven expression of 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.DepositorInsertEEA-5guides-PGK-Puro
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-SOCS1
Plasmid#174571PurposeHuman HA-tagged SOCS1 expression (N-term. tag) (CMV prom.)DepositorInsertHA-SOCS1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLIX402 PHF2
Plasmid#91700Purposeinducible lentiviral expression of PHF2DepositorInsertPHF2 (PHF2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
Myc-tagged delta N (dominant-negative form) human TCF4E pcDNA3
Plasmid#32739DepositorInsertTCF4E (Tcf7l2 Human)
UseTagsMycExpressionMammalianMutationThe N-terminal deletion form (delta N) lacks amin…PromoterAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR
Plasmid#224435PurposeAAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.DepositorInsertsmScarlet-KASH
U6-gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
PWPXLD-EGFR-WT
Plasmid#133749PurposeWild-type EGFR tagged with HA cloned into lentiviral PWPXLD plasimdDepositorInsertWild type EGFR-HA (EGFR Human)
UseLentiviralTagsHA tagExpressionMammalianMutationPromoterEF1-alphaAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hHNF4alpha
Plasmid#206878PurposeThe ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the Bam HI/XbaI sites of the pcDNA3.1(+), the mammalian expression vector.DepositorInsertHuman hepatocyte nuclear factor 4 alpha (HNF4A Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag hHMGB1
Plasmid#31609DepositorInsertHMGB1 (HMGB1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
LBR-mCherry
Plasmid#172446PurposeExpression of nuclear envelope protein LBR with a C-terminal mCherry tagDepositorInsertlamin B receptor (LBR Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cytosolic Syk Biosensor
Plasmid#125729PurposeFRET biosensor for studying SYK activities in cellsDepositorInsertSYK biosensor (ZAP70 Synthetic)
UseTags6xHistine Tag at the N-terminal.ExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 EGFP-TEADi
Plasmid#140145PurposeTetracycline-inducible TEADi (GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal)DepositorInsertEGFP TEADi
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
P2RY4-Tango
Plasmid#66475PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertP2RY4 (P2RY4 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHM6-alphasynuclein-A53T
Plasmid#40825DepositorInsertSNCA (SNCA Human)
UseTags6x His and HAExpressionMammalianMutationA53TPromoterCMVAvailable sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
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SPLICS PM-ER Long P2A
Plasmid#164111PurposeDetect the long Plasma membrane-Endoplasmic reticulum contactDepositorInsertSplit YFP PM-ER Long P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCG-Hc∆18
Plasmid#84817PurposeMeasles virus hemagglutinin expressing plasmidDepositorInsertMeasles virus H glycoprotein
UseTagsExpressionMammalianMutationPromoterCMV IEAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only
