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80,559 results

Showing: 3841 - 3880 of 80559 results

  1. APEX2-OMM

    Plasmid
    #79056
    Purpose
    Soybean APEX2 anchored to cytosolic face of outer mitochondrial membrane with C-terminal targeting sequence of last 31aa of human mitochondrial antiviral-signaling protein
    Depositor
    Alice Ting
    Article
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Insert
    Soybean APEX2
    Use
    Lentiviral
    Tags
    flag and last 31 amino acids of human MAVS
    Expression
    Bacterial and Mammalian
    Promoter
    CMV immearly promotor
    Available Since
    Aug. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    79056-plasmid-map-sequence-id-238450

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  3. pLenti6MQBI163 GLUT2

    Plasmid
    #194138
    Purpose
    Expresses GLUT2 protein in mammalian cells
    Depositor
    Jun Liu
    Insert
    SLC2A2 (SLC2A2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194138-plasmid-map-sequence-id-380702

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  4. tdTomato-VE-Cadherin-N-10

    Plasmid
    #58142
    Purpose
    Localization: Tight Junctions, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    VE-Cadherin (CDH5 Human)
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    I503T in VE-Cadherin
    Promoter
    CMV
    Available Since
    Nov. 19, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. TFORF3187

    Plasmid
    #144663
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RAD21 (RAD21 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144663-plasmid-map-sequence-id-350018

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  18. pCMV-T7-ABE8.20m-nSpRY-P2A-EGFP (KAC1335)

    Plasmid
    #185917
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8.20m-nSpRY-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185917-plasmid-map-sequence-id-367357

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  20. CCR6-DuET

    Plasmid
    #213202
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR6 (CCR6 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213202-plasmid-map-sequence-id-430765

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  22. EBFP2-Beta-Catenin-20

    Plasmid
    #55235
    Purpose
    Localization: Tight Junctions, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Insert
    Beta-Catenin (Ctnnb1 Mouse)
    Tags
    EBFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. mTLR1

    Plasmid
    #13080
    Depositor
    Ruslan Medzhitov
    Insert
    TLR1 (Tlr1 Mouse)
    Expression
    Mammalian
    Available Since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only

  29. TFORF0021

    Plasmid
    #141480
    Purpose
    Lentiviral vector for overexpressing the IRX1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    IRX1 (IRX1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141480-plasmid-map-sequence-id-276576

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  30. mPlum-Lysosomes-20

    Plasmid
    #55982
    Purpose
    Localization: Lysosome Membrane, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Insert
    LAMP1 (Lamp1 Rat)
    Tags
    mPlum
    Expression
    Mammalian
    Mutation
    D50E
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    55982-plasmid-map-sequence-id-147991

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  33. pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

    Plasmid
    #201916
    Purpose
    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Inserts
    Gag-Cas9 v2
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    Expression
    Mammalian
    Promoter
    CAG and Human U6
    Available Since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201916-plasmid-map-sequence-id-403306

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Showing: 3841 - 3880 of 80559 results