4,298 results
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Plasmid#136375PurposeGateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is usedDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only
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pDGB3_omega1
Plasmid#68238PurposeGoldenBraid level 1 Omega plant expression vectorDepositorInsertempty vector
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha2
Plasmid#68229PurposeGoldenBraid level 1 Alpha plant expression vector.DepositorInsertempty vector
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJY-RpABE-FT_INF
Plasmid#112874Purposebinary vector for INF (Induced Neo-Functionalization) of FT in ArabidopsisDepositorInsertsRPS5A promoter
ABE7.10
U6-sgRNA targeting FT
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB165 - pL1R3_pMAS::rLUC-I::tMAS
Plasmid#123198Purposebinary plant vector for transient expression of a Renilla luciferase (rLUC, with intron)DepositorInsertpMAS::rLUC-I::tMAS
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPTXcGMPRELUC
Plasmid#68503Purposecyclic nucleotide reporter for bacteria or plant cellsDepositorInsertOPTX promoter with cGMPRE (AT1G33440 Mustard Weed)
UseTagsluciferaseExpressionBacterial and PlantMutation3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…PromoterOPTX promoter with cGMPREAvailable sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-H840A_1
Plasmid#91162PurposeCas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:hpt IIDepositorInsertAtCas9_H840A (nickase)
UseCRISPRTagsExpressionPlantMutationH840APromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pAct-AtINT2
Plasmid#127529PurposePlasmid encodes A. thaliana codon optimized Integrase 2.DepositorInsertIntegrase 2 coding sequence codon optimized for A. thaliana expression.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pAct-AtINT5
Plasmid#127531PurposePlasmid encodes A. thaliana codon optimized Integrase 5.DepositorInsertIntegrase 5 coding sequence codon optimized for A. thaliana expression.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pAct-AtINT7
Plasmid#127532PurposePlasmid encodes A. thaliana codon optimized Integrase 7.DepositorInsertIntegrase 7 coding sequence codon optimized for A. thaliana expression.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pAct-AtINT9
Plasmid#127533PurposePlasmid encodes A. thaliana codon optimized Integrase 9.DepositorInsertIntegrase 9 coding sequence codon optimized for A. thaliana expression.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S(rc)2_4_5-GFP
Plasmid#127520PurposePlasmid has an inverted CaMV 35S promoter sequence (reverse complement) flanked by attB and attP attachment sites of integrases 2, 4, and 5. EGFP coding sequence is in the forward orientation.DepositorInsertCaMV 35S reverse complement promoter sequence flanked by attB/attP Integrase 2, 4 and 5 attachment sites.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
MIGS5.2 (FF576)
Plasmid#35254DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGWB14-LYX001
Plasmid#126219PurposeWith a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.DepositorInsertNiben101Scf00149g02001.1
UseTagsHAExpressionPlantMutationPromoterCaMV 35SAvailable sinceJune 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ284-v1
Plasmid#138118PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with D172R mutation, without promoterDepositorInsertMb2Cas12a v1
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationD172RPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
P16ΔS:(MCS)-3xHA-S11
Plasmid#108185PurposeBinary vectors used for the constitutive expression of tripartite split-sfGFP components (with a 3xHA tag) in plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterP16ΔSAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDGB1a2R+pNOS_Red-F_NOSt
Plasmid#170888PurposeGoldenBraid Transcriptional Unit - pNOS_Red-F_NOSt Reverse orientationDepositorInsertRed-F
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationp.S286Y Red mutantPromoterpNOSAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVL44 - pVND7>>
Plasmid#116010Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpVND7:GR-LhG4:tVND7:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFH88
Plasmid#128201PurposesgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)DepositorInsertsgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtGLV6:T35s
Plasmid#164741PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMedtr3g095180.1
UseTagsTerminator 35SExpressionPlantMutationPromoterLotus japonicus UbiquitinAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtGLV2:T35s
Plasmid#164740PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMedtr5g012400.1
UseTagsTerminator 35SExpressionPlantMutationPromoterLotus japonicus UbiquitinAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSC2
Plasmid#91180PurposeT-DNA vector for combinatorial deletion of NCR genes in medicago truncatula (Csy4 array of 6 gRNAs under the control of CmYLCV promoter)DepositorInsertgRNAs targeting NCR genes
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMB-TMEg
Plasmid#61209PurposeFluorescent reporter for plasma membrane and trunk peri-arbuscular membrane MtBCPsp-GFP-BCP expressed under MtBCP1 promoterDepositorInsertMtBCPsp-GFP-BCP
UseTagsEGFPExpressionPlantMutationPromoterMtBCP1Available sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMB-TLEr
Plasmid#61188PurposeFluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under MtBCP1 promoterDepositorInsertMtRAB7A2
UseTagsmCherryExpressionPlantMutationPromoterMtBCP1Available sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZA57
Plasmid#174770PurposeIn planta gene expressionDepositorInsertGG_NbZAR1syn-4xMyc, Kmr plant selection marker
UseTags4xMycExpressionPlantMutationWTPromoterAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292 (AaCas12b)-D570A-TV
Plasmid#136380PurposeCRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation systemDepositorInsertAaCas12b (D570A)-TV
UseCRISPRTagsExpressionPlantMutationD570APromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX029
Plasmid#167152PurposeMoClo-compatible Level 1 (position 3) vector encoding Neonothopanus nambi hispidin-3-hydroxylase nnH3H under control of 0.4 kb 35S promoter, for expression in plantsDepositorInsertp35s-nnH3H-ocsT
UseTagsExpressionPlantMutationPromoterp35sAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
P315-FBP_10
Plasmid#139701PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing CPH and NpgaDepositorInsertpUBQ1:NnH3H:tNos, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM829.2
Plasmid#84158Purposetarget miRNA829.2 for destruction in Arabidopsis thalianaDepositorInsertSTTM829.2
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUPD2+pAtUbq10
Plasmid#170877PurposePhytobrick - pAtUBQ10DepositorInsertpAtUBQ10
UseTagsExpressionPlantMutation441C>T to remove BbsI restriction sitePromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2+NtEUt
Plasmid#170883PurposePhytobrick - NtEUtDepositorInsertNtEUt
UseTagsExpressionPlantMutationPromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD+AtHSP18.2t
Plasmid#170884PurposePhytobrick - AtHSP18.2tDepositorInsertHSP18.2t
UseTagsExpressionPlantMutationPromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB216
Plasmid#68607PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsNuclear localization signal-tagged tdTomato (tdTo…ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMDC32-FIT2
Plasmid#96990PurposeExpress mouse FIT2 gene in plants.DepositorInsertMmFIT2 (Fitm2 Mouse)
UseTagsExpressionPlantMutationPromoter35SAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC32-SEIPIN3
Plasmid#96975PurposeExpress Arabidopsis SEIPIN3 gene in plants.DepositorInsertSEIPIN3 (AT2G34380 Mustard Weed)
UseTagsExpressionPlantMutationPromoter35SAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB401+Citrine
Plasmid#128432PurposeModified R4pGWB401 three way Gateway destination vector (Nakagawa et al. 2008) that enables C-terminal fusion of Citrine, a dimeric acid stable yellow fluorescent protein.DepositorTypeEmpty backboneUseGateway destination vectorTagsCitrine, yellow fluorescence proteinExpressionPlantMutationPromoterAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHWBF06exp
Plasmid#130831PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertSUN2
UseTagsmCherry-FLAG-HAExpressionPlantMutationPromoterCaMV 35SAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18
Plasmid#172317PurposeCRISPR dCas9 directly fused to a catalytic inactive version of bacterial DNA methyltransferase MQ1 to target to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(C141S,S317A)_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
TF101-STTM-miR165/166
Plasmid#84816PurposeTurning off/down miR165/166 in SoybeanDepositorInsertSTTM-miR165/166
UseTagsExpressionPlantMutationPromoterunknownAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB423
Plasmid#68688PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTriple repeats of Citrine (3xCitrine)ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTX208
Plasmid#89268PurposeExpress STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA01 and OsYSA-gRNA02DepositorInsertOsYSA-gRNA01 and OsYSA-gRNA02
UseCRISPRTagsSV40 NLSExpressionPlantMutationPromoterMaize Ubiquitin1 promoterAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC43-SEIPIN2
Plasmid#96977PurposeExpress GFP-tagged Arabidopsis SEIPIN2 gene in plants.DepositorInsertSEIPIN2 (AT1G29760 Mustard Weed)
UseTagsGFPExpressionPlantMutationPromoter35SAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC43-SEIPIN1
Plasmid#96976PurposeExpress GFP-tagged Arabidopsis SEIPIN1 gene in plants.DepositorInsertSEIPIN1 (AT5G16460 Mustard Weed)
UseTagsGFPExpressionPlantMutationPromoter35SAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:ER-GFP1–10
Plasmid#125664PurposeBinary vector for the inducible expression of ER-targeted GFP1–10 fragment that can be used to detect a protein tagged with GFP11DepositorInsertER-GFP1–10
UseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-dead_1
Plasmid#91165PurposeCas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:hpt IIDepositorInsertAtCas9_D10A+H840A (dead)
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
MIGS4.2 (FF575)
Plasmid#35252DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLEELA
Plasmid#79752Purposebinary DEST vector for plant expressionDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter2xPro35SAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCO489
Plasmid#139732PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, LuzDepositorInsertpMOD_D_AtuMas:NnLuz:trbcSE9
UseLuciferaseTagsExpressionPlantMutationPromoterAtuMASAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0058
Plasmid#117539PurposeLevel1 Golden Gate Cassette: sgRNA cassette for SaCas9, targeting NbPDS gene in N. benthamianaDepositorInsertsgRNA (SaCas9)
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0040
Plasmid#117607PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_NbPDS(pEPOR1CB0058)DepositorInsert[35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only