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Showing: 151 - 200 of 3935 results

  3. pGTMV

    Plasmid
    #118755
    Purpose
    Binary plasmid with a tobacco mosaic virus infectious clone; Agrobacterium-mediated transformation required for expression in plants.
    Depositor
    Jose-Antonio Daros
    Article
    Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10
    Insert
    Tobacco mosaic virus
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Cauliflower mosaic virus 35S
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    118755-plasmid-map-sequence-id-229554

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  5. pJL-TRBO

    Plasmid
    #80082
    Purpose
    An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence.
    Depositor
    John Lindbo
    Article
    Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    80082-plasmid-map-sequence-id-358589

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  9. pHRE

    Plasmid
    #134909
    Purpose
    Overexpression of recombinant proteins in plants thanks to efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Test UTR combinations by swapping 5' and 3' UTRs.
    Depositor
    George Lomonossoff
    Article
    Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    double 35S
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    134909-plasmid-map-sequence-id-267026

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  20. pYPQ203 (pMDC32-Ubi1)

    Plasmid
    #86207
    Purpose
    Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromiycin resistance marker
    Depositor
    Yiping Qi
    Article
    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10
    Type
    Empty backbone
    Use
    Gateway compatible attr1-attr2 destination vector
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Maize ubiquitin 1 (ZmUbi1)
    Available since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86207-plasmid-map-sequence-id-174284

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  21. pGW_RUBY_EggCas9

    Plasmid
    #225990
    Purpose
    To clone entry vector with CRISPR guide RNA via Gateway cloning; it has an Egg cell-specific expression of Cas9 and the plant is selected by the red color due to constitutive expression of RUBY
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    225990-plasmid-map-sequence-id-448019

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  22. pDIRECT_10E

    Plasmid
    #91209
    Purpose
    Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers , Plant Selection: none
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91209-plasmid-map-sequence-id-182248

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  24. pCHW90004

    Plasmid
    #222436
    Purpose
    Level 0, promoter module (GGAG-CCAT) containing CBS4 and tomato miniDFR promoters.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Insert
    CBS4-miniDFR, CBS4 promoter + miniDFR minimal promoter (Solanum lycopersicum)
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    no
    Promoter
    Available since
    Nov. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    222436-plasmid-map-sequence-id-452566

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  26. P307-FBP_6

    Plasmid
    #139697
    Purpose
    T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway
    Depositor
    Daniel Voytas
    Article
    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1
    Insert
    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    various
    Available since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    139697-plasmid-map-sequence-id-296513

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  30. pCBC-DT2T3

    Plasmid
    #50591
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T2, gRNA scaffold, U6-29t plus, U6-1p, T3
    Use
    CRISPR; Pcr template
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50591-plasmid-map-sequence-id-244476

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  32. ePPEplus

    Plasmid
    #205241
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Zong Yuan
    Article
    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:
    Insert
    c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Detailed in manuscript
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    205241-plasmid-map-sequence-id-408831

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  39. pEG302 22aa SunTag VP64 nog

    Plasmid
    #120251
    Purpose
    CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    NOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    120251-plasmid-map-sequence-id-238812

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  42. pLaNGo11-7

    Plasmid
    #126055
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x7 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126055-plasmid-map-sequence-id-247152

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  46. pKb-TnpB2

    Plasmid
    #215334
    Purpose
    ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI site
    Depositor
    Kutubuddin Molla
    Article
    Karmakar et al Plant Biotechnol J. 2024 Jun 28. doi: 10
    Insert
    Rice codon optimized ISDra2 TnpB
    Use
    Tags
    Not applicable
    Expression
    Plant
    Mutation
    Promoter
    Rice ubiquitin promoter for TnpB and maize ubiqui…
    Available since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    215334-plasmid-map-sequence-id-447325

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  47. pDGB3alpha2_35S:P19:Tnos (GB1203)

    Plasmid
    #68214
    Purpose
    Transcriptional unit for Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression driven by the 35S promoter
    Depositor
    Diego Orzaez
    Article
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    P19 silencing suppressor
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    35S
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68214-plasmid-map-sequence-id-119066

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  48. pM-Cas12a

    Plasmid
    #196291
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding LbCas12a in place of viral GP
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding LbCas12a in place of viral GP (cas12a Synthetic)
    Use
    CRISPR
    Tags
    Flag
    Expression
    Plant
    Mutation
    See depositor comments below
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196291-plasmid-map-sequence-id-389824

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  50. CMPE-ePPEplus

    Plasmid
    #205242
    Purpose
    For multiplex plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Zong Yuan
    Article
    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:
    Insert
    Csy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Detailed in manuscript
    Promoter
    Available since
    Oct. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    205242-plasmid-map-sequence-id-408832

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Showing: 151 - 200 of 3935 results