8,135 results

1. pJK148-Pnmt41-GBP-mCherry(N)-leu1+

   Plasmid

   #89070

   Purpose

   genomic GBP-mCherry epitope N-terminal tagging at leu1+ locus in S. pombe

   Depositor

   Quanwen Jin

   Article

   Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

   Type

   Empty backbone

   Use

   Genomic integration in s. pombe

   Tags

   GBP-mCherry

   Expression

   Yeast

   Available Since

   June 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYD1

   Plasmid

   #73447

   Purpose

   expression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cells

   Depositor

   Dane Wittrup

   Article

   Kieke et al Protein Eng. 1997 Nov;10(11):1303-10.

   Type

   Empty backbone

   Tags

   6xHis, AGA2, T7 peptide, V5 tag, and Xpress tag

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-mCherry-hphMX6

   Plasmid

   #105156

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu

   Article

   Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

   Insert

   mCherry-hphMX6
   

   Tags

   mCherry

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS416-yZ3EV-Z3pr-yEGFP (RB3579)

   Plasmid

   #69100

   Purpose

   URA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr driving expression of yEGFP

   Depositor

   David Botstein

   Inserts

   yZ3EV cassette
   Z3 promoter
   yEGFP
   

   Expression

   Yeast

   Available Since

   Sept. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-6xGLY-3xFLAG-HIS3MX6

   Plasmid

   #20753

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Expression

   Yeast

   Available Since

   March 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pV1393

   Plasmid

   #111430

   Purpose

   CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR_XPR2_hrGFP

   Plasmid

   #84614

   Purpose

   HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into XPR2 locus

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   hrGFP
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   UAS1B8-TEF(136)

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPRyl_XPR2

   Plasmid

   #84609

   Purpose

   Introduces DSB at XPR2 locus in Y. lipolytica

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Promoter

   UAS1B8-TEF(136)

   Available Since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR_AXP_hrGFP

   Plasmid

   #84613

   Purpose

   HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into AXP locus

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   hrGFP
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   UAS1B8-TEF(136)

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPRyl_AXP

    Plasmid

    #84608

    Purpose

    Introduces DSB at AXP locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR_D17_hrGFP

    Plasmid

    #84616

    Purpose

    HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into D17 locus

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    hrGFP
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR_MFE1_hrGFP

    Plasmid

    #84617

    Purpose

    HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into MFE1 locus

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    hrGFP
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPRyl_D17

    Plasmid

    #84611

    Purpose

    Introduces DSB at D17 locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPRyl_A08

    Plasmid

    #84610

    Purpose

    Introduces DSB at A08 locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPRyl_MFE1

    Plasmid

    #84612

    Purpose

    Introduces DSB at MFE1 locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR_A08_hrGFP

    Plasmid

    #84615

    Purpose

    HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into A08 locus

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    hrGFP
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYY10-2µ-TP901

    Plasmid

    #235771

    Purpose

    2µ plasmid for constitutive expression of TP901 under RNR2 promoter.

    Depositor

    Chang Liu

    Article

    Pisera et al bioRxiv [Preprint]. 2024 Nov 30:2024.11.

    Insert

    TP901 integrase
    

    Expression

    Yeast

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJRoC30_Tv9nL

    Plasmid

    #188069

    Purpose

    PROSS-designed high-redox potential laccase from Trametes versicolor

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

    Insert

    PROSS-designed high-redox potential laccase from Trametes versicolor
    

    Tags

    S. cerevisiae evolved α-factor prepro-leader sign…

    Expression

    Yeast

    Mutation

    79 PROSS mutations, described in publication

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRS416-Gal4-dCas9-VP64

    Plasmid

    #71128

    Purpose

    This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.

    Depositor

    Ronald Davis

    Article

    Naranjo et al PLoS Genet. 2015 Dec 29;11(12):e1005751.

    Insert

    Gal4-dCas9-VP64
    

    Tags

    NLS n-terminal, N terminal Gal4 Activator domain,…

    Expression

    Yeast

    Promoter

    Tef1

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRW404-2µ-BXB1

    Plasmid

    #235976

    Purpose

    2µ plasmid for constitutive expression of BXB1 under RNR2 promoter.

    Depositor

    Chang Liu

    Article

    Pisera et al bioRxiv [Preprint]. 2024 Nov 30:2024.11.

    Insert

    BXB1
    

    Expression

    Yeast

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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