8,025 results

1. pYES2-Ub-M-GFP

   Plasmid

   #11952

   Depositor

   Nico Dantuma

   Article

   Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

   Insert

   Ub-M-GFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Ubiquitin fused to N-terminus of GFP. Methionine …

   Promoter

   Available since

   May 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS403-HIS3-pINO4-SV40NLS-PfV-GS-Sapphire

   Plasmid

   #203658

   Purpose

   Yeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm GEMs under the HIS3 promoter.

   Depositor

   Liam Holt

   Article

   Shu T et et al bioRxiv 2022

   Insert

   PfV
   

   Use

   Tags

   Sapphire

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBIS-GALkFLP(URA)

   Plasmid

   #49460

   Purpose

   overexpression of Flp step-arrest mutant in yeast, URA selection

   Depositor

   Marc Gartenberg

   Article

   Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

   Insert

   Flp(H305L) (flp Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H305L, ‘step-arrest’ mutant,

   Promoter

   GAL10

   Available since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. FRP2008

   Plasmid

   #159312

   Purpose

   Yeast tagging vector to create gene fusion with yeast codon optimized mKOkappa under KanMX selection

   Depositor

   Joerg Stelling

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   V2del

   Promoter

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG305GPD-ccdB

   Plasmid

   #14138

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU

   Plasmid

   #64180

   Purpose

   harbours marker gene URA3

   Depositor

   Markus Ralser

   Article

   Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Aug. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAG426GPD-ccdB-HA

   Plasmid

   #14252

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   3HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG303GAL-ccdB

   Plasmid

   #14133

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRS416Gal TDP43 WT YFP

   Plasmid

   #27447

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pWS171

    Plasmid

    #90518

    Purpose

    Cas9 gap repair vector - LEU2

    Depositor

    Tom Ellis

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENO1-dTom-NATr

    Plasmid

    #52208

    Purpose

    Constitutive expression of Candida codon-optimized dTomato with NAT selectable marker

    Depositor

    Robert Wheeler

    Article

    Gratacap et al Dis Model Mech. 2013 Sep;6(5):1260-70. d

    Insert

    dTomato
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for Candida species

    Promoter

    ENO1

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pYSD313

    Plasmid

    #212931

    Purpose

    Encodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    Sed1 (SED1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pESC-LEU (V5 and HA)

    Plasmid

    #222486

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and LEU2 selection

    Depositor

    Kiyofumi Yamada, Tetsuo Matsuzaki

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    HA and V5

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. UASGAL-Pgal

    Plasmid

    #60338

    Purpose

    Contains a promoter of activating sequence derived from native galactose promoter coupled with full length native galactose promoter

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pEK36

    Plasmid

    #210598

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    SpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter
    

    Use

    Tags

    mClover3

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpH2Bpro

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTEF6-7

    Plasmid

    #124583

    Purpose

    Reporter plasmid with constitutively expressed mCherry and YFP (eCitrine)

    Depositor

    Matthias Heinemann

    Article

    Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pE1n

    Plasmid

    #17441

    Purpose

    modified pENTR vector for N-terminal STREP tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    STREP

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDR-GW AT1.03YEMK

    Plasmid

    #28004

    Depositor

    Wolf Frommer

    Article

    Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

    Insert

    AT1.03YEMK
    

    Use

    Tags

    mVenus and mseCFP

    Expression

    Yeast

    Mutation

    M60Y, G131E, K132M, 133K

    Promoter

    Available since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAV125

    Plasmid

    #63191

    Purpose

    pRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGS_129

    Plasmid

    #160652

    Purpose

    Expresses Human APOE4 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

    Depositor

    Susan Lindquist, Li-Huei Tsai

    Article

    Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

    Insert

    Apolipoprotein E e4 (APOE Human)
    

    Use

    Tags

    Kar2 signal sequence

    Expression

    Yeast

    Mutation

    e4 allele

    Promoter

    pZ estradiol inducible promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDZ274 (pLEU MET25pro MCP-2x-yeGFP)

    Plasmid

    #45929

    Depositor

    Robert Singer, Daniel Zenklusen

    Article

    Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

    Insert

    MCP-2x-yeGFP
    

    Use

    Tags

    2x-yeGFP

    Expression

    Yeast

    Mutation

    Promoter

    MET25

    Available since

    Aug. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

22. 12URA-B-ScTop2∆CTD

    Plasmid

    #214924

    Purpose

    Yeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)

    Depositor

    James Berger

    Article

    Jeong et al Elife. 2022 Nov 7;11:e81786. doi: 10.755

    Insert

    topoisomerase II
    

    Use

    Tags

    6x His-TEV

    Expression

    Yeast

    Mutation

    Deleted amino acids 1178-1428

    Promoter

    Gal 1/10

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pUG-natNT2

    Plasmid

    #110922

    Purpose

    expression of nat gene conferring resistance to nourseothricin for gene deletion in yeast

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    nat
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    AgTEF1

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGTy1mhis3-AI or pGTy1his3-AI

    Plasmid

    #62228

    Purpose

    A Ty1 element tagged with the retrotransposition indicator gene his3-AI is fused to the GAL1 promoter on a multicopy URA3-based shuttle plasmid.

    Depositor

    David Garfinkel

    Article

    Curcio et al Proc Natl Acad Sci U S A. 1991 Feb 1;88(

    Insert

    Ty1H3 retrotransposon
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Feb. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pZ_P-AOX1-eGFP

    Plasmid

    #126718

    Purpose

    yeast plasmid overexpressing eGFP

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    egfp
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCfB2909(XII-5 MarkerFree)

    Plasmid

    #73281

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-5 (Chr XII: 839226..840357)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. YIplac211-yoHaloTag-TLG1

    Plasmid

    #105277

    Purpose

    Plasmid for pop-in/pop-out replacement of Tlg1 with yoHaloTag-Tlg1.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Tlg1 (TLG1 Budding Yeast)
    

    Use

    Tags

    yoHaloTag

    Expression

    Yeast

    Mutation

    HaloTag has yeast optimized codons

    Promoter

    Available since

    Jan. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAP01

    Plasmid

    #59981

    Purpose

    The function of this material is to facilitate the capture and expression of bacterial gene clusters.

    Depositor

    Bradley Moore

    Article

    Yamanaka et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

    Type

    Empty backbone

    Use

    Synthetic Biology; Transformation-associated reco…

    Tags

    no

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    no

    Available since

    Nov. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGI3EM22C

    Plasmid

    #135488

    Purpose

    Agrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hph

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    hph SpunH2A/Bpr LifeAct-tdTomato
    

    Use

    Tags

    LifeAct

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunH2A/Bpr (divergent)

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTW040

    Plasmid

    #64769

    Purpose

    For integration of Cre recombinase at the yeast HIS3 locus orthe CYC1 terminator

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    Cre-EBD78
    

    Use

    Tags

    fused to the human Estrogen Binding Domain (EBD),…

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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