8,173 results
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Plasmid#51567PurposeConstitutively express PM (Plasma membrane) localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
TagsCAAX and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable SinceAug. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAV0761
Plasmid#133494PurposeExpression of nucleo-cytoplasmic mTagBFP2 in P-cellsDepositorInsertpLys3(BstZ17I)-p(map3)-mTagBFP2-terminator(ScADH1)-bleMX
ExpressionYeastAvailable SinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLK
Plasmid#64186Purposeharbours marker genes LEU2 and LYS2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pESC-NAT-LACap
Plasmid#80763PurposeExpresses truncated L-A capsid for curing satellite virusDepositorInsertL-A cDNA (L-A Cap)
ExpressionYeastPromoterPGK1Available SinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pYZ293
Plasmid#102687PurposeS. pombe gRNA entry vector with Cas9 - Sp leu1 markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJS288
Plasmid#200714PurposeTo express yeast Hxt1 hexose transporter in yeastDepositorUseSynthetic BiologyTagsmCherryExpressionYeastMutationdomesticated for MoClo cloning (removal of intern…Available SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSP-G2
Plasmid#64737PurposeYeast expression plasmidDepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-Sec31-yoHalo
Plasmid#115423PurposeExpress Sec31-yoHalo at the endogenous locus via pop-in pop-out mutagenesisDepositorInsertSec31-yoHalo
TagsyoHaloTagExpressionYeastPromoterEndogenousAvailable SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
FRP2372
Plasmid#127578PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Lys2 selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorExpressionYeastAvailable SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIM573
Plasmid#74637Purposealias pFA6a-ymTag-BFP-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and G418 selection markerDepositorInsertymTagBFP
ExpressionYeastPromoternoneAvailable SinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-ymTurquoise2-CaURA3
Plasmid#118458PurposeYeast tagging vector to create a gene fusion with yeast optimized mTq2 with CaUra3 selection. *An updated version of this plasmid is available: #168057 pFA6a-link-ymTq2-URA3.DepositorInsertymTurquoise2
ExpressionYeastAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG306GPD-ccdB-DsRed
Plasmid#14356DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-fSNAP-3xHA
Plasmid#188935PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertURA-GAL1-fSNAP-3xHA
ExpressionYeastAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
3413 pBF339 Bcl-XL
Plasmid#8773DepositorInsertBcl-XL (Bcl2l1 Mouse)
ExpressionYeastAvailable SinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pE3n
Plasmid#17457Purposemodified pENTR vector for N-terminal 6x MYC tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTags6xMycExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pScDD2_ERdd
Plasmid#109047Purposedestabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).DepositorInsertER50 (ESR1 H. sapiens gene with yeast enhanced codon)
Tagsyeast enhanced GFP with 8 amino acids TSGRGEGQ li…ExpressionYeastMutation3 mutations : E380G, R434G, N532SPromoterSOD1Available SinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
p413TEF-PKM2
Plasmid#34608DepositorAvailable SinceJan. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
ZJOM22
Plasmid#133629PurposeIntegration of EMC1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast endoplasmic reticulum (ER).DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-ADH700-yeCherry-YAP1-yeGFP-DHFR
Plasmid#24549DepositorInsertpADH1 yeCherry::YAP1 yeGFP-DHFR (YAP1 Budding Yeast)
ExpressionYeastMutation1. Truncated version of ADH700 promoter (SbfI/Spe…Available SinceMay 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNK5867
Plasmid#219751PurposepGAP-like vector encoding hispidin-synthase from Mycena citricolor under control of GAP promoter, for yeast expressionDepositorInsertpGAP - mcitHispS - tAOX
ExpressionYeastAvailable SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK1508
Plasmid#219750PurposepGAP-like vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of GAP promoter, for yeast expressionDepositorInsertpGAP - npgA - tAOX
ExpressionYeastAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
TetO7-HSP30UTR-nLucPEST-MS2(v4)
Plasmid#199764PurposeControl construct of elongation reporter to quantify elongation of LacZDepositorInsertNanolucPEST
ExpressionYeastPromoterTetO7Available SinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Gal1/10p CAS9 Gal1/10p I-SceI
Plasmid#182519PurposeGalactose-Induced Expression vector for Cas9 and I-SceI.DepositorInsertsI-SceI
Cas9
ExpressionYeastAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
PP78
Plasmid#78948PurposeSupplemental Figure 3. Integration Site 5.DepositorInsertIntegration Site 5
ExpressionYeastAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV0584
Plasmid#133471PurposeIntegration into fission yeast genome at ura4 locus with natMX selection cassetteDepositorTypeEmpty backboneExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIW447-CRISPR XYL2
Plasmid#98906PurposeK. marxianus CRISPR Plasmid for XYL2 editingDepositorInsertsCodon optimized Cas9
sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsSV40ExpressionYeastAvailable SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKG-GW1
Plasmid#203163PurposeYeast expression vector; To express proteins under control of TDH3 promoter and terminator using leucine selectionDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJZC638
Plasmid#62283PurposeExpresses MCP-VP64 and dCas9 in Yeast cellsDepositorInsertsMCP-VP64
dCas9
TagsVP64ExpressionYeastMutationNuclease activity has been deactivated by mutatio…PromoterpAdh and pGal10Available SinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB6371
Plasmid#106153PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6630, integration into Yarrowia lipolytica chromosomal location IntC_3, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymYPET
Plasmid#118455PurposeOverexpression of ymYPETDepositorInsertymYPET
ExpressionYeastMutationYPET A206K, F208S, E232L, N235DAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPTK010-3a-Glucoamylase-αMFΔ
Plasmid#84969PurposeType 3a, Glucoamylase followed by αMFΔ - secretion signalDepositorInsertGlucoamylase followed by αMFΔ secretion tag
UseSynthetic BiologyExpressionYeastPromoterNonAvailable SinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pG/ER(G)
Plasmid#108219PurposeYeast expression vector for human estrogen receptor alphaDepositorAvailable SinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG306GAL-ccdB
Plasmid#14139DepositorTypeEmpty backboneUseGateway destinationExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
TetO7-HSP30UTR-LacZ-PKTlinker-nLucPEST-MS2(v4)
Plasmid#199763PurposeElongation reporter construct to quantify elongation of LacZDepositorInsertLacZ-nLucPEST
ExpressionYeastPromoterTetO7Available SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a- mYFP-kanMX6
Plasmid#105147PurposeYeast gene targetingDepositorInsertmYFP-kanMX6
TagsmYFPExpressionBacterial and YeastMutationA206KAvailable SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pI1-MTU-DO-G418
Plasmid#160012PurposeIntegrative vector for K. marxianus, targeting the LAC4 locus. G418 selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yECFP-Hygro
Plasmid#44647DepositorInsertyEmCFP
ExpressionYeastAvailable SinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTH732-2µ-RLuc/staCFLuc
Plasmid#40607DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationThe last three codons of the full-length Firefly …PromoterADH1 and TDH3Available SinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET8
Plasmid#107439PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIM23
Plasmid#74643PurposeCentromeric plasmid to construct episomal C-terminal fusions of genes with yeast-adapted TagRFP-T and HIS3 selection markerDepositorInsertyTagRFP-T
ExpressionYeastPromoterMET25Available SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS306-pHIS3-2xGFP-tURA3
Plasmid#116936PurposeYeast integrative vector for the expression of 2xGFPDepositorInsert2xGFP
ExpressionYeastPromoterHIS3Available SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS267
Plasmid#122380PurposeCRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codonDepositorInsertHSP90
UseCRISPRExpressionYeastAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-his3-P81nmt1-3FLAG
Plasmid#19341DepositorInserthis3 (his3 Fission Yeast)
ExpressionYeastAvailable SinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCfB8786
Plasmid#126920PurposePlasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtIDepositorInsertXdCrtI
ExpressionYeastAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-Alpha-synuclein-H50Q
Plasmid#181724PurposeGalactose inducible expression of Alpha-synuclein-H50QDepositorAvailable SinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMJA103
Plasmid#128519PurposeExpresses human amyloid Lysozyme in Pichia pastorisDepositorAvailable SinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pATP403red-hENT1
Plasmid#166027PurposeExpresses human equilibrative nucleoside transporter 1 (hENT1)DepositorInserthuman equilibrative nucleoside transporter 1 (SLC29A1 Human)
UseSynthetic BiologyExpressionYeastPromoterpTDH3Available SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVG1
Plasmid#111444PurposeUnified Solo vector pV1382 + sgScADE2 + ScADE2 stop codon repair tempateDepositorInsertCaCas9/sgScADE2/stop-codon repair
UseCRISPRExpressionYeastAvailable SinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG423GAL-ccdB-EGFP
Plasmid#14197DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only