7,933 results
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Plasmid#184965PurposeTest effect of extending -Eco1 a1/a2 in yeastDepositorInsertEco1: RT and ncRNA(extended), a1/a2 length: 27
UseTagsExpressionYeastMutationhuman codon optimized RT, a1/a2 length extended t…PromoterGal7Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-natMX6-P41nmt1-3FLAG
Plasmid#19346DepositorInsertnatMX6
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-ccdB-DsRed
Plasmid#14364DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pIMVAd40R
Plasmid#98309PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of GAL promoters; integrated into ERG9 locus;DepositorInsertERG9C-terminal-TURA3- PGAL7>MVD1>TPRM9-PGAL2>-ERG12>TNATTIDP1UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBBK01 pCAS-Tyr-[gRNA: BsaI GFP dropout] (KanR, AmpR)
Plasmid#178985PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
YEp112-CUP1-His-Smt3
Plasmid#99537PurposeYeast episomal vector for expression of His-tagged Smt3 (SUMO); TRP1 markerDepositorInsertSMT3 (SMT3 Budding Yeast)
UseTags7xHisExpressionYeastMutationPromoterCUP1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
MET-HYGRO
Plasmid#218113PurposeIntegration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LYS-Leu2
Plasmid#218114PurposeIntegration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY2
Plasmid#218115PurposeActivity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY3
Plasmid#218116PurposeActivity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY3-LYS-IV
Plasmid#218119PurposeLYS-Leu2 plasmid (218114) with the functional cassettes of pY3 (218116) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pAV0782
Plasmid#133514PurposeExpression of sfGFP tagged LifeAct probe to monitor actin cytoskeletonDepositorInsertpAde6(PmeI)-p(act1)-LifeAct-sfGFP-terminator(ScADH1)-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf-E2-Crimson
Plasmid#117658PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-pro-alphaf-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFTK020
Plasmid#171292PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertPalcA syn_NoCrea (ANIA_08979) promoter
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-NUbo-GFP-His6
Plasmid#212790PurposeCopper-inducible expression of the artificial test substrate NUbo-GFP-His6 in budding yeastDepositorInsertNUbo-GFP-His6
UseIntegrative vectorTagsExpressionYeastMutationPromoterCUP1Available sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACT2.2gtwy
Plasmid#11346DepositorInsertgateway cassette
UseTagsGal4 Activation DomainExpressionYeastMutationPromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
M2660 ura3::LYS2 Disruptor Converter
Plasmid#51678PurposeYeast marker swap plasmid for converting URA3 to LYS2DepositorInsertLYS2 (LYS2 Budding Yeast)
UseYeast marker swapTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
yHS1
Plasmid#159150PurposeCytosolic labile heme reporterDepositorInsertyHS1
UseTagsExpressionYeastMutationPromoterGPDAvailable sinceSept. 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYC34
Plasmid#63915PurposeRecipient overexpressing vector to construct mCherry tagged fusionsDepositorTypeEmpty backboneUseTagsmCherryExpressionYeastMutationPromoterTEF1 (Saccharomyces cerevisiae)Available sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYES2-Ub-R-GFP
Plasmid#11953DepositorInsertUb-R-GFP
UseTagsExpressionYeastMutationUbiquitin fused to N-terminus of GFP. Arginine at…PromoterAvailable sinceMay 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
p426 46Q GPD
Plasmid#1182DepositorInserthtt 46Q (HTT Human)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pXNubWtgate
Plasmid#105080PurposeYeast mating-based Split Ubiquitin Assay, positive controlDepositorTypeEmpty backboneUseTagsNubIExpressionYeastMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPEE47
Plasmid#139426PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPEE46
Plasmid#139425PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.DepositorInsertmRuby3
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-MNN9-msGFP2
Plasmid#177105PurposeExpresses Mnn9-msGFP2 in yeast cellsDepositorInsertMNN9 (MNN9 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKmK.C3
Plasmid#125061PurposeStorage plasmid for K.marxianus centromereDepositorInsertKmARS/CEN6
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDS1885
Plasmid#177341PurposeExpresses Aequorin in Candida albicans using the dominant marker SAT1DepositorInsertAequorin
UseTagsExpressionYeastMutationPromoterACT1Available sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS12-mCherry
Plasmid#25444DepositorInsertmCherry
UseTagsCOX4 PresequenceExpressionYeastMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMTU-DO-HPH-C4
Plasmid#160343PurposeHigh-copy expression vector for K. marxianus, hygromycin selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pOst1-pro-alphaf(MUT1)-E2-Crimson
Plasmid#117662PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpOst1-pro-af(MUT1)-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG413 GAL ORF1 Shuffle1 NLS
Plasmid#145794PurposemPing Yeast Transposition AssayDepositorInsertORF1 Shuffle1 NLS
UseTagsExpressionYeastMutationEnhanced Nucleur Localization SignalPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG425 GAL Pong TPase L418A, L420A
Plasmid#145795PurposemPing Yeast Transposition AssayDepositorInsertPong TPase L418A, L420A
UseTagsExpressionYeastMutationL418A, L420APromoterAvailable sinceJan. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
p415 Glo3
Plasmid#112651PurposeExpression S.cerevisiaeDepositorInsertGlo3
UseTagsExpressionYeastMutationPromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT82_GalL_nCDA1Δ198-BE3
Plasmid#145046PurposeExpresses nCDA1Δ198-BE3 in yeast cellsDepositorInsertnCDA1Δ198-BE3
UseCRISPRTagsExpressionYeastMutationpmCDA1(1-198AA); spCas9(D10A)PromoterGalLAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXP842
Plasmid#46068DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterADH2Available sinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pETCON-PTX-S1-220
Plasmid#67747PurposePertussis Toxin Subunit 1 Yeast Display PlasmidDepositorInsertPTxS1-220
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-ymYPET-CaURA3
Plasmid#118460PurposeYeast tagging vector to create a gene fusion with ymYPET with CaUra3 selectionDepositorInsertymYPET
UseTagsExpressionYeastMutationYPET A206K, F208S, E232L, N235DPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
CuGFP-ATG8(416)
Plasmid#49423PurposeExpresses ATG8 fused at the N terminus to GFP under the control of the CUP1 promoter.DepositorInsertautophagy-related 8 (ATG8 Budding Yeast)
UseTagsGFPExpressionBacterial and YeastMutationPromoterCUP1Available sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet26
Plasmid#107448PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMet26 (met26 Fission Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet16
Plasmid#107452PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMet16 (met16 Fission Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZaa-STE2-cohis
Plasmid#133433PurposeYeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.DepositorInsertSTE2 (STE2 Budding Yeast)
UseTagsExpressionYeastMutationC59S/C252A, Codon optimized for P. pastoris, and …PromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS425ADS
Plasmid#20119DepositorInsertamorphadiene synthase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMarch 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yETSapphire-CaUra3
Plasmid#44650DepositorInsertTurbo Sapphire
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAV0328
Plasmid#133489PurposeExpression of nucleo-cytoplasmic mCherryDepositorInsertpUra4(AfeI)-p(act1)-mCherry-terminator(tdh1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP2_leu2d_NRT1.1
Plasmid#58332PurposeExpresses Arabidopsis thaliana NRT1.1 in Saccharomyces cells as a C-terminal GFP his tagged proteinDepositorInsertNRT1.1 (NRT1.1 Mustard Weed)
UseTagsGFP-HisExpressionYeastMutationPromoterGAL1Available sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB778
Plasmid#60154PurposeGreen click beetle luciferase (Promega) driven by LEU1 promoter.DepositorInsertCBG99
UseTagsExpressionYeastMutationPromoterLEU1prAvailable sinceNov. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
YIp LYS2-LEU2
Plasmid#122909PurposeLYS2-LEU2 integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
p415GPD-3xHA-GW
Plasmid#63781Purposeyeast expression vector p415GPD modified such that it is Gateway converted and introduces a 3xHA tag at the N-terminus of the insertDepositorTypeEmpty backboneUseTags3xHAExpressionYeastMutationPromoterGPDAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only