8,265 results
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Plasmid#179064PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-VAM3, a marker for the vacuoleDepositorInsertRPL18Bp-Scarlet(rfp)-Link-VAM3
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-OSBP PH domain
Plasmid#58840PurposeExpresses GFP-Tagged OSBP PH domain in yeastDepositorAvailable SinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pK3FS
Plasmid#85776PurposeN-ICE plasmid pK3FSDepositorInsertloxP-KanMX-loxP-3FLAG
UseCre/LoxExpressionYeastAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
1x_a1gRNAOp_pCYC1m_yeBFP2
Plasmid#64390Purposeencodes one a1 operator site upstream of minimal pCYC1 promoter driving yeast enhanced BFPDepositorInsertyeast enhanced BFP2
ExpressionYeastAvailable SinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGAN201
Plasmid#129204PurposeyTRAP sensor of [RNQ+], detects aggregation of Rnq1DepositorAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymiRFP680-URA3
Plasmid#224102PurposeYeast tagging vector to create a gene fusion with yeast optimized miRFP680 with URA3 selectionDepositorInsertymiRFP680
TagsymiRFP680ExpressionYeastAvailable SinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymiRFP680-Kan
Plasmid#224090PurposeYeast tagging vector to create a gene fusion with yeast optimized miRFP680 with Kan selectionDepositorInsertymiRFP680
TagsymiRFP680ExpressionYeastAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymiRFP680-SpHis5
Plasmid#224095PurposeYeast tagging vector to create a gene fusion with yeast optimized miRFP680 with SpHis5 selectionDepositorInsertymiRFP680
TagsymiRFP680ExpressionYeastAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yemiRFP670-URA3
Plasmid#224100PurposeYeast tagging vector to create a gene fusion with yeast optimized emiRFP670 with URA3 selectionDepositorInsertyemiRFP670
TagsyemiRFP670ExpressionYeastAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRG714_lexO-AscI_LexA-TF_URA3MX
Plasmid#154822PurposeInducible expression of AscI endonuclease in S. cerevisiaeDepositorInsertsAscI endonuclease
LexA-ER-B112
TagsFLAG and NLSExpressionYeastPromoterACT1 and lexO_4-CYC1_coreAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2B-57
Plasmid#177927PurposeExpresses yeGFP under control of doxycycline-responsive TET3 promoter (contains three rtTA binding sites)DepositorArticleInsertEGFP
ExpressionBacterial and YeastPromoterTET3 (contains three rtTA binding sites)Available SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA-Ub-G76A-Rnq1-GFP
Plasmid#21052DepositorAvailable SinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pALD4 T-
Plasmid#161481PurposeNon targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THi11_T-_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Non targeting control NTC)
ExpressionYeastPromoterpALD4, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJW1234
Plasmid#62367PurposepRS305 pPGK1-BirA::LEU2DepositorAvailable SinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
Designed VEGF-binding scFv G6des1
Plasmid#110212PurposeYeast Surface Display of G6des1DepositorInsertG6des1
Tagscmyc tagExpressionYeastMutationL A43P, L Y49F, L Y55F ,L Q89L, L T94V, L P95T, L…Available SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB4787
Plasmid#106147PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSMF2
Plasmid#112819PurposeExpresses wild-type Rpb1 in yeast cellsDepositorAvailable SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mMaple3-kanMX6
Plasmid#105165PurposeYeast gene targetingDepositorInsertmMaple3-kanMX6
TagsGlySerGly-mMaple3ExpressionBacterial and YeastAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pABY-c46
Plasmid#201774PurposeExpresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeastDepositorInsertNbALFA
Tags40aa Linker, mNeonGreenExpressionYeastPromoterTEF1Available SinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT AID*-GFP osTIR1
Plasmid#182514PurposeC-SWAT compatible vector for C-terminal tagging with AID*-GFP for auxin inducible gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration.DepositorInsertAID*
TagsGFPExpressionYeastAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS306-PTDH3-SUMO10-6SIM
Plasmid#190248PurposeExpression SUMO10-SIM6 in yeast cellsDepositorInsert10SUMO-6SIM
ExpressionYeastAvailable SinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS426-GFP-Osh2-PH
Plasmid#71373PurposeFor analysis of PH domain localization in yeastDepositorInsertOsh2-PH
TagsEGFPExpressionYeastMutationPleckstrin homology (PH) domain; aa 277-398Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDB4279
Plasmid#98698PurposeA control plasmid containing the intact bsdMX markerDepositorInsertCas9
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG306-GPD-empty chr I
Plasmid#41895DepositorTypeEmpty backboneExpressionYeastPromoterGPDAvailable SinceApril 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fSNAP-URA
Plasmid#188917PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fSNAP-URA
ExpressionYeastAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB2791
Plasmid#63654PurposepCfB2791 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxExpressionYeastPromoterTEF1 promoterAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pESC-ARG (V5 and HA)
Plasmid#222493PurposeCo-expression of two genes in yeast with V5 and HA tags and ARG1 selectionDepositorTypeEmpty backboneTagsHA and V5ExpressionYeastAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-TYR
Plasmid#222484PurposeCo-expression of two genes in yeast with FLAG and MYC tags and TYR1 selectionDepositorTypeEmpty backboneTagsFLAG and MycExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-MET (V5 and HA)
Plasmid#222490PurposeCo-expression of two genes in yeast with V5 and HA tags and MET15 selectionDepositorTypeEmpty backboneTagsHA and V5ExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-LYS (V5 and HA)
Plasmid#222491PurposeCo-expression of two genes in yeast with V5 and HA tags and LYS2 selectionDepositorTypeEmpty backboneTagsHA and V5ExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-TYR (V5 and HA)
Plasmid#222492PurposeCo-expression of two genes in yeast with V5 and HA tags and TYR1 selectionDepositorTypeEmpty backboneTagsHA and V5ExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-THR (V5 and HA)
Plasmid#222494PurposeCo-expression of two genes in yeast with V5 and HA tags and THR1 selectionDepositorTypeEmpty backboneTagsHA and V5ExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-THR
Plasmid#222479PurposeCo-expression of two genes in yeast with FLAG and MYC tags and THR1 selectionDepositorTypeEmpty backboneTagsFLAG and MycExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-ADE
Plasmid#222480PurposeCo-expression of two genes in yeast with FLAG and MYC tags and ADE2 selectionDepositorTypeEmpty backboneTagsFLAG and MycExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-ARG
Plasmid#222481PurposeCo-expression of two genes in yeast with FLAG and MYC tags and ARG1 selectionDepositorTypeEmpty backboneTagsFLAG and MycExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
ymTq2-yeVenus pDRF1-GW
Plasmid#168076PurposeFRET fusion between ymTq2 and yeVenus for use in yeastDepositorInsertymTq2-yeVenus
ExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJW1663
Plasmid#112037PurposeGal-Estradiol transcription activation expression cassette marked with KanRDepositorInsertGal DNA binding-estradiol binding-MSN2 activator
ExpressionYeastPromoterADH1Available SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
416Gal-FUS-R521C-YFP
Plasmid#29622DepositorAvailable SinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGAN147
Plasmid#129202Purposeyeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backboneDepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastPromoterSUP35Available SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-EGFP
Plasmid#166143PurposeEGFP expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.DepositorInsertEGFP
ExpressionYeastPromoterGAL1Available SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-mRuby3
Plasmid#166144PurposemRuby3 expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.DepositorInsertmRuby3
ExpressionYeastPromoterGAL1Available SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV0752
Plasmid#133486PurposeExpression in fission yeast from promoter pnmt41 , integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(nmt41)-sfGFP-terminator(ScCYC1)
ExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG306-GPD-AVT1 chr I
Plasmid#41898PurposeYeast expression of AVT1.DepositorAvailable SinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoGFPgamma-SpHis5
Plasmid#44859PurposeYeast tagging vector to create gene fusion with yeast optimized GFPgamma with SpHis5 selectionDepositorInsertyoGFPgamma
ExpressionYeastAvailable SinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHES840
Plasmid#89191PurposeExpresses YFP under control of GAL1 promoter.DepositorInsertpGAL1-YFP
ExpressionYeastPromoterGAL1Available SinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
YEp112-CUP1-His-Smt3
Plasmid#99537PurposeYeast episomal vector for expression of His-tagged Smt3 (SUMO); TRP1 markerDepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-SAT1
Plasmid#162465PurposepFA-MNase-CaURA3 was double digested to remove the URA3 auxotrophy marker. The SAT1 marker was amplified from pFA-SAT1 and cloned into the AscI-SacI-digested pFA-MNaseDepositorInsert3FLAG-Mnase
ExpressionYeastAvailable SinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIPlac204TC-NLS-DsRed-Express2
Plasmid#25443DepositorInsertNLS-DsRed-Express2
TagsNLSExpressionYeastAvailable SinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRS426-HsFECH
Plasmid#174524PurposeExpresses human ferrochelatase (FECH) in yeastDepositorInsertFECH with yeast HEM15 promoter and terminator (FECH Human, Synthetic, Budding Yeast)
ExpressionYeastMutationLacks original mitochondrial targeting sequence (…PromoterHEM15 (yeast, 257 bp)Available SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG425GAL-ccdB-HA
Plasmid#14249DepositorTypeEmpty backboneUseGateway destinationTags3HAExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only