8,350 results
-
Plasmid#51682PurposeYeast marker swap plasmid for converting URA3 to KanMX3DepositorInsertKanMX3
UseYeast marker swapExpressionYeastAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPTK009-3a-αAmylase-αMFΔ
Plasmid#84968PurposeType 3a, α-Amylase followed by αMFΔ - secretion signalDepositorInsertα-Amylase followed by MFΔ secretion tag
UseSynthetic BiologyExpressionYeastPromoterNonAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mRFP1-kanMX6
Plasmid#133551PurposeYeast fluorescent (RFP) vector with PTEF promoter.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIMVAd40R
Plasmid#98309PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of GAL promoters; integrated into ERG9 locus;DepositorInsertERG9C-terminal-TURA3- PGAL7>MVD1>TPRM9-PGAL2>-ERG12>TNATTIDP1ExpressionYeastAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
p415GPD-3xHA-GW
Plasmid#63781Purposeyeast expression vector p415GPD modified such that it is Gateway converted and introduces a 3xHA tag at the N-terminus of the insertDepositorTypeEmpty backboneTags3xHAExpressionYeastPromoterGPDAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
KBB418
Plasmid#185088PurposeSwi6L-GFP with nuclear export signal mutated to alaninesDepositorInsertSWI6
TagsGFPExpressionYeastMutationSwi6 nuclear export signal L to A mutationsAvailable SinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB460
Plasmid#185094PurposeNMA111-GFP wild type under endogenous promoterDepositorInsertNMA111
TagsGFPExpressionYeastMutationNMA111-GFPAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
EZ-L580
Plasmid#173023PurposeEncodes OptoAMP4 circuitDepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastMutationA79QAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJZC620
Plasmid#62282PurposeExpresses dCas9, MCP-VP64, and PCP-VP64 in Yeast cellsDepositorInsertsMCP-VP64
PCP-VP64
dCas9
TagsVP64ExpressionYeastMutationNuclease activity has been inactivated by mutatio…PromoterpAdh and pTdh3Available SinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMEL10
Plasmid#107916PurposeURA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRExpressionYeastAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYX113-mtGFP
Plasmid#45049DepositorInsertmtGFP
ExpressionYeastPromoterGALAvailable SinceMay 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG425GPD-ccdB-DsRed
Plasmid#14370DepositorTypeEmpty backboneUseGateway CloningTagsDsRedExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
RIB VP64 R1
Plasmid#161493PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R1_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting R1 in the RIB1 promoter)
ExpressionYeastPromoterpGAP, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXP416
Plasmid#26842DepositorTypeEmpty backboneExpressionYeastAvailable SinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMM0638
Plasmid#129005PurposeIntegration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2DepositorInsert5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1
ExpressionYeastAvailable SinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS306-pHIS3-2xGFP-tURA3
Plasmid#116936PurposeYeast integrative vector for the expression of 2xGFPDepositorInsert2xGFP
ExpressionYeastPromoterHIS3Available SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICE URA3
Plasmid#122882PurposeURA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable SinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-OSBP PH domain
Plasmid#58840PurposeExpresses GFP-Tagged OSBP PH domain in yeastDepositorAvailable SinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mScarlet-H-mNeonGreen-kanMX (pKBJ009)
Plasmid#173456PurposeContains mScarletH-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-H-mNeonGreen
ExpressionYeastPromoterno promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAH243
Plasmid#121147PurposeExpression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeastDepositorInsertshumanized Streptococcus pyogenes Cas9
gRNA expression module
UseCRISPRTagsFlagExpressionYeastPromoterS.pombe rrk1 and nmt41Available SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
12URA-B-ScTop2∆CTD
Plasmid#214924PurposeYeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)DepositorInserttopoisomerase II
Tags6x His-TEVExpressionYeastMutationDeleted amino acids 1178-1428PromoterGal 1/10Available SinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWKS10
Plasmid#139417PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Clover marker.DepositorInsertClover
ExpressionYeastPromoterTEF1Available SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-ccdB-DsRed
Plasmid#14364DepositorTypeEmpty backboneUseGateway CloningTagsDsRedExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pWS3867
Plasmid#185744PurposeSub-array backbone 3/4DepositorTypeEmpty backboneExpressionYeastAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS303 Gal TDP-43 YFP
Plasmid#27468DepositorAvailable SinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
ZJOM156
Plasmid#133636PurposeIntegration of 2xGFP tag at SEC7 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.DepositorAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB2196
Plasmid#67549PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked bleMX marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJW1666
Plasmid#112040PurposeCEN/ARS plasmid for GAL-inducible empty reporter V5-TEV-EGFP tagged expression marked with URADepositorInsertNone
TagsV5-TEV-EGFPExpressionYeastPromoterGAL1Available SinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIp128-P30-His-POL30
Plasmid#99542PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA); LEU2 markerDepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJZC533
Plasmid#135435PurposeYeast integrating plasmid for pTEF1-mCherry reporter geneDepositorInsertmCherry
ExpressionYeastMutationcodon optimized for S. cerevisiaePromoterpTEF1Available SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM1
Plasmid#133632PurposeIntegration of GFP-SED5 as an additional copy in genome, use auxotrophic marker URA3(Kluyveromyces lactis). Marker for yeast early golgi.DepositorAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1234
Plasmid#62367PurposepRS305 pPGK1-BirA::LEU2DepositorAvailable SinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGFP-ATG13(315)
Plasmid#122072PurposeExpressing GFP-Atg13DepositorInsertATG13
TagsGFPExpressionYeastAvailable SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRG645_lexO-AscI_LexA-TF_LEU2MX
Plasmid#154813PurposeInducible expression of AscI endonuclease in S. cerevisiaeDepositorInsertsAscI endonuclease
LexA-ER-B112
TagsFLAG and NLSExpressionYeastPromoterACT1 and lexO_4-CYC1_coreAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-natMX6-P3nmt1-3FLAG
Plasmid#19345DepositorInsertnatMX6
ExpressionYeastAvailable SinceSept. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCfB8786
Plasmid#126920PurposePlasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtIDepositorInsertXdCrtI
ExpressionYeastAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUK
Plasmid#64185Purposeharbours marker genes URA3 and LYS2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
1x_a1gRNAOp_pCYC1m_yeBFP2
Plasmid#64390Purposeencodes one a1 operator site upstream of minimal pCYC1 promoter driving yeast enhanced BFPDepositorInsertyeast enhanced BFP2
ExpressionYeastAvailable SinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTH732-2µ-RLuc/staCFLuc
Plasmid#40607DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationThe last three codons of the full-length Firefly …PromoterADH1 and TDH3Available SinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA-Ub-G76A-Rnq1-GFP
Plasmid#21052DepositorAvailable SinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJZC603
Plasmid#62317PurposesgRNA with 2x PP7 for yeast cellsDepositorInsertsgRNA + 2x PP7 RNA binding module
ExpressionYeastPromoterSNR52Available SinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKT0210
Plasmid#8724DepositorInsertyECFP A206R
TagsCodon optimized linkerExpressionYeastMutationyEGFP with F64L, S65T, Y66W, N146I, M153T, V163A,…Available SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pNXRVc-HACre
Plasmid#78400Purposeconstitutive Cre expression vectorDepositorInserts3xHA-nls-Cre
natMX6
ExpressionYeastAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
ZJOM11
Plasmid#133637PurposeIntegration of GFP-TLG1 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT130_GalL_nCDA1-NGBE3
Plasmid#145094PurposeExpressing base editor nCDA1-NGBE3 in yeast cellsDepositorInsertnCDA1-NGBE3
UseCRISPRExpressionYeastMutationspCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…PromoterGalLAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoGFPgamma-SpHis5
Plasmid#44859PurposeYeast tagging vector to create gene fusion with yeast optimized GFPgamma with SpHis5 selectionDepositorInsertyoGFPgamma
ExpressionYeastAvailable SinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-Mup1-mScarlet2I
Plasmid#140152PurposePlasmid for pop-in/pop-out replacement of Mup1 with Mup1-mScarlet2IDepositorInsertMUP1
ExpressionYeastAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-HyTkAX
Plasmid#73898Purposefission yeast drug counter-selectable selection markerDepositorInserthyg-TK
ExpressionYeastAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVG47
Plasmid#164705PurposeyEVenus under the control of tetOprDepositorInserttetOpr - yEVenus - CLN2 PEST - ADH1t
ExpressionYeastAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGAN201
Plasmid#129204PurposeyTRAP sensor of [RNQ+], detects aggregation of Rnq1DepositorAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only