8,265 results
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Plasmid#83554PurposeUsed to evaluate the expression output of ADH2 promoter with yEGFP used as the reporter gene.DepositorInsertADH2 promoter-yEGFP
ExpressionYeastPromoterADH2Available SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPS2348
Plasmid#1351DepositorAvailable SinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pDN0604 (pDEST, HygR)
Plasmid#210483PurposepDEST vector with HygR marker for gene expression in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…ExpressionYeastPromoterADH1Available SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN0603 (pDEST, NatR)
Plasmid#210482PurposepDEST vector with NatR marker for gene expression in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…ExpressionYeastPromoterADH1Available SinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCu-MCP-2yeGFP(405)
Plasmid#121419PurposeExpressing MCP-2yeGFPDepositorInsertCUP1p-MCP-2yeGFP
ExpressionYeastPromoterCUP1 promoterAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG303GPD-ccdB-EGFP
Plasmid#14182DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
7851 pUG72-HSC82in
Plasmid#10894DepositorAvailable SinceApril 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCRA03
Plasmid#103141PurposeTriple gRNA expression separated with 28nt Csy4 motifDepositorInsertgRNAs
ExpressionYeastAvailable SinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
GAL FLAG46QDProGFP p306
Plasmid#15832DepositorInserthtt 46Q Delta Pro (HTT Human)
TagsFLAG and GFPExpressionYeastMutationProline-rich region removedAvailable SinceOct. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBBK01 pCAS-Tyr-[gRNA: BsaI GFP dropout] (KanR, AmpR)
Plasmid#178985PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mTID-HA3-KanMX6
Plasmid#227982PurposeTo insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID, KanMX6 markerDepositorInsertminiTurboID
TagsHA tagExpressionYeastAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLexA::Ras
Plasmid#11340DepositorAvailable SinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pM
Plasmid#64179Purposeharbours marker gene MET17DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
p413TEF-SirT1
Plasmid#51746Purposeoverexpression of human SirT1 in S. cerevisiae, HIS3 markerDepositorAvailable SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
ZJOM103
Plasmid#133664PurposeIntegration of SEC7-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.DepositorAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG303GAL-ccdB-HA
Plasmid#14229DepositorTypeEmpty backboneUseGateway destinationTags3HAExpressionYeastAvailable SinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046708
Plasmid#149173PurposeYeast Expression plasmid for SARS-CoV-2 nsp4DepositorInsertSARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAG426-GAL-Alpha-synuclein-A53T
Plasmid#181722PurposeGalactose inducible expression of Alpha-synuclein-A53TDepositorAvailable SinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-fCLIP-3xHA
Plasmid#188936PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertURA-GAL1-fCLIP-3xHA
ExpressionYeastAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pREF-GFP kan
Plasmid#122061Purposereporter plasmid for termination and terminator override to express GFP. Marked with URA3DepositorInsertsintergenic terminator from yeast IMD2
green fluorescent protein
ExpressionYeastPromoterGAL1 and GAL1 comes in pYES2Available SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
LHP1463 - INP52
Plasmid#32432DepositorAvailable SinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
YIp ADE2-LEU2
Plasmid#122905PurposeADE2-LEU2 integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable SinceOct. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046203
Plasmid#145488PurposeYeast Expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
TagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
YIplac211-VPS8-HaloTagx2
Plasmid#177113PurposeExpresses Vps8-HaloTagx2 in yeast cellsDepositorInsertVPS8 (VPS8 Budding Yeast)
ExpressionYeastAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
ZJOM96
Plasmid#133663PurposeIntegration of SEC26-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.DepositorAvailable SinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
5538 pUG2-SSA1
Plasmid#1247DepositorInsertSsa1 (SSA1 Budding Yeast)
ExpressionYeastAvailable SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pDN-T2dMFot
Plasmid#44558DepositorInsertsPTETREG promoter
rtetR-M2::FFF
UseSynthetic Biology; Expression regulator/reporterExpressionYeastMutationCompared to rtTA, rtTA-M2 has S12G, E19G, A56P, D…PromoterPTETREGAvailable SinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
LD209
Plasmid#69182PurposeIntegration of 2GFP tag at Atg9 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg9-2EGFP
Tags2xEGFPExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-backbone
Plasmid#172517PurposeYeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertionDepositorInsertgRNA cassette
UseCRISPRExpressionYeastPromoterSNR52Available SinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 FL
Plasmid#163574Purposeexpress wild type Dcp2 at endogenous level in S.cerevisiaeDepositorAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSN061
Plasmid#101725PurposeSingle copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsS. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastPromoterHeterologous TEF1 promoter from A. gossypii. and …Available SinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUD628
Plasmid#103018Purposeexpression of a Cpf1 programming crRNA targetting ADE2 (crADE2-3.S)DepositorAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
EZ-L439
Plasmid#169146PurposeEncodes OptoINVRT7 circuitDepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV105
Plasmid#63195PurposepRS405 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
M3938 trp1::ADE2 Disruptor Converter
Plasmid#51673PurposeYeast marker swap plasmid for converting TRP1 to ADE2DepositorAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pINER5
Plasmid#185895PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
ExpressionYeastMutationNoneAvailable SinceAug. 25, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pETconNK-TEM1(S70A,D179G)
Plasmid#81166PurposepETconNK with TEM1(S70A,D179G) beta-lactamaseDepositorInsertTEM-1 beta-lactamase
Tagsc-mycExpressionBacterial and YeastMutationDeleted the sec transport tag. Plasmid encodes H2…PromoterGAL1 and gal1Available SinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYC34
Plasmid#63915PurposeRecipient overexpressing vector to construct mCherry tagged fusionsDepositorTypeEmpty backboneTagsmCherryExpressionYeastPromoterTEF1 (Saccharomyces cerevisiae)Available SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMC-XI2
Plasmid#176156Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-2DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pI1-MTU-DO-HIS
Plasmid#160142PurposeIntegrative vector for K. marxianus, targeting the LAC4 locus. Histidine selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDS1885
Plasmid#177341PurposeExpresses Aequorin in Candida albicans using the dominant marker SAT1DepositorInsertAequorin
ExpressionYeastPromoterACT1Available SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG423GAL-ccdB-HA
Plasmid#14245DepositorTypeEmpty backboneUseGateway destinationTags3HAExpressionYeastAvailable SinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPTK020-7-attB
Plasmid#84989PurposeType 7, BxbI recognition siteDepositorInsertBxbI recognition site
UseSynthetic BiologyExpressionYeastMutationBsaI site removed (1711 t to a)PromoterNonAvailable SinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJZC519
Plasmid#62278PurposeYeast dCas9-VP64 expression plasmidDepositorInsertdCas9-VP64
TagsHas a VP64 fusionExpressionYeastMutationHas mutations in the RuvC1 and HNH domains to ren…PromoterpTdh3Available SinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
YIp ADE2-HphMx
Plasmid#122916PurposeADE2-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable SinceOct. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAH5
Plasmid#41829PurposeUsed as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for hygromycin resistance.DepositorTypeEmpty backboneUsePcr templateExpressionBacterial and YeastPromoterT7,TEFAvailable SinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG423GPD-ccdB-HA
Plasmid#14246DepositorTypeEmpty backboneUseGateway destinationTags3HAExpressionYeastAvailable SinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
Gma-pCAMBIA-1301-STTM171
Plasmid#84080PurposeDisturb the expression of miR171 in soybeanDepositorInsertmiR171
ExpressionBacterial, Plant, and Yeast…Promoter2x35SAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPTK006-3a-αMF no EAEA
Plasmid#84965PurposeType 3a, α-mating factor no EAEA - secretion signalDepositorInsertα-mating factor no EAEA secretion tag
UseSynthetic BiologyExpressionYeastPromoterNonAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKar2-msGFP-HDEL
Plasmid#117668PurposeLocalisation of Endoplasmic ReticulumDepositorInsertpKar2-msGFP-HDEL
ExpressionYeastMutationContains the Kar2 promoter and signal sequence fr…PromoterpKar2Available SinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only