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8,350 results

Showing: 2751 - 2800 of 8350 results

  2. pMOD8A-iRGR-r11

    Plasmid
    #74369
    Purpose
    This plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.
    Depositor
    Eric Klavins
    Article
    Gander et al bioRxiv 041871
    Insert
    RGR-r11
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    AHD1
    Available Since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    74369-plasmid-map-sequence-id-141324

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  3. pDRF'- AmTryoshka1;3-LS-F138I-T78H

    Plasmid
    #100963
    Purpose
    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    AmTryoshka1;3-LS-F138I-T78H (AMT1;3 Mustard Weed)
    Expression
    Yeast
    Mutation
    F138I, T78H
    Promoter
    PMA
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100963-plasmid-map-sequence-id-193742

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  15. pPS293

    Plasmid
    #8851
    Depositor
    Pamela Silver
    Insert
    GAL1 promoter (GAL1 Budding Yeast)
    Expression
    Yeast
    Available Since
    Aug. 3, 2005
    Availability
    Academic Institutions and Nonprofits only

  19. pCoofy42

    Plasmid
    #55190
    Purpose
    Pichia pastoris expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Tags
    His10-Stop-OneStrep-Stop-S-tag-Stop-CBP-HPC4-Stop…
    Expression
    Yeast
    Promoter
    AOX1
    Available Since
    July 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    55190-plasmid-map-sequence-id-85572

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  22. p404MET3

    Plasmid
    #17419
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    MET3 promoter + polylinker + CYC1 terminator (MET3 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    MET3 promoter is between SacI and XbaI restrictio…
    Available Since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17419-plasmid-map-sequence-id-8594

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  27. pRS314-hUD

    Plasmid
    #11005
    Depositor
    Francisco Malagon
    Insert
    hUD recombination system
    Expression
    Bacterial and Yeast
    Mutation
    Direct repeat recombination system. Recombination…
    Available Since
    April 28, 2006
    Availability
    Academic Institutions and Nonprofits only
    11005-plasmid-map-sequence-id-6839

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  28. pDIV494

    Plasmid
    #177701
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    sgRNA Targeting ADE2 locus in D.hansenii: AGCTAAGCAGATTAATGCAT
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Promoter
    RNR2p (Debaryomyces hansenii ), SNR52p (Candida s…
    Available Since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177701-plasmid-map-sequence-id-352589

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  32. HsSPHK2

    Plasmid
    #118092
    Purpose
    Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)
    Depositor
    Kevin Lynch
    Article
    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do
    Insert
    Homo sapiens Sphingosine Kinase 2 (SPHK2 Human)
    Tags
    FLAG
    Expression
    Yeast
    Promoter
    GAL1, 10
    Available Since
    Aug. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    118092-plasmid-map-sequence-id-234125

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  33. HCP5

    Plasmid
    #166107
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNAs that targets the C-terminus of Cyr1.
    Depositor
    Matthias Heinemann
    Insert
    Cyr1CT-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166107-plasmid-map-sequence-id-330320

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  36. pGBW-m4046464

    Plasmid
    #148970
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pGAL1
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    148970-plasmid-map-sequence-id-297202

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  38. LHP336 - HA-Ub-K48R

    Plasmid
    #32164
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Tags
    HA
    Expression
    Yeast
    Mutation
    K48R
    Promoter
    CUP1
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  40. pPS808

    Plasmid
    #8856
    Depositor
    Pamela Silver
    Type
    Empty backbone
    Tags
    GFP
    Expression
    Yeast
    Available Since
    Aug. 3, 2005
    Availability
    Academic Institutions and Nonprofits only

  46. pGBW-m4046310

    Plasmid
    #149022
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Tags
    Cleavable thrombin;MBP
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149022-plasmid-map-sequence-id-291475

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  47. pGBW-m4046423

    Plasmid
    #148998
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 nsp7
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Tags
    Cleavable thrombin;MBP
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    148998-plasmid-map-sequence-id-297676

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Showing: 2751 - 2800 of 8350 results