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Showing: 1 - 50 of 1251 results

  3. pCAG-lox-rox-STOP-rox-lynScarlet-lox-Lyn-NeonGreen

    Plasmid
    #175259
    Purpose
    dual plasmid labelling membrane target protein of different neuronal population with different flurophore
    Depositor
    Sebastián Dupraz, Frank Bradke
    Article
    Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37
    Insert
    Lox-rox-STOP-rox-lyn-mScarlet-pA-lox
    Use
    Cre/Lox; Dre/rox
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    None (wt)
    Promoter
    pCAG
    Available since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    175259-plasmid-map-sequence-id-375583

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  5. pET28a_LYN

    Plasmid
    #224238
    Purpose
    Expression of human LYN Kinase in E. coli
    Depositor
    Andrew Mesecar
    Insert
    lyn (LYN Human)
    Use
    Tags
    6xHis and 6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promotor
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    224238-plasmid-map-sequence-id-458419

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  9. pFastBacHTa_LYN

    Plasmid
    #224244
    Purpose
    Expression of human LYN Kinase in insect cells (SF9 cells)
    Depositor
    Andrew Mesecar
    Insert
    lyn (LYN Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    224244-plasmid-map-sequence-id-458379

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  11. Lyn-R-GECO1

    Plasmid
    #120410
    Purpose
    Expresses a fusion between myristoylation signal peptide and R-GECO1. This construct targets R-GECO1 to the plasma membrane.
    Depositor
    Won Do Heo
    Article
    Kim et al Proc Natl Acad Sci U S A. 2016 May 24;11
    Insert
    Lyn-R-GECO1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    120410-plasmid-map-sequence-id-247900

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  16. Lyn-tdnano-HA

    Plasmid
    #136512
    Purpose
    Mammalian expression of a tandem dimer of WT SspB (nano), targeted to membrane by Lyn myristoylation sequence, with triple-HA epitope tag
    Depositor
    Bianxiao Cui
    Article
    Hope et al J Mol Biol. 2020 Jun 12;432(13):3739-374
    Insert
    Lyn nano nano 3HA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    136512-plasmid-map-sequence-id-271004

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  21. Lyn11-BirA*-HA

    Plasmid
    #214474
    Purpose
    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with an N-terminal Lyn11 plasma membrane localization sequence and C-terminal HA tag.
    Depositor
    Natalie Ahn
    Article
    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.
    Insert
    BirA(R118G)
    Use
    Lentiviral; Gateway
    Tags
    HA tag and Lyn11 (Plasma Membrane Localization Se…
    Expression
    Mammalian
    Mutation
    R118G
    Promoter
    EF-1a
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214474-plasmid-map-sequence-id-425600

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  29. pcDNA3-Lyn-dInPAkt

    Plasmid
    #181834
    Purpose
    Genetically encoded single-color sensor for monitoring plasma membrane PI(3,4,5)P2 dynamics in living cells. Based on dimerization-dependent red fluorescent protein (ddRFP).
    Depositor
    Jin Zhang
    Article
    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.
    Insert
    Lyn-dInPAkt
    Use
    Tags
    N-terminal targeting sequence from Lyn kinase, dd…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181834-plasmid-map-sequence-id-364316

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  30. pcDNA3-Lyn-dPlcR

    Plasmid
    #181832
    Purpose
    Genetically encoded single-color sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells. Based on dimerization-dependent green fluorescent protein (ddGFP).
    Depositor
    Jin Zhang
    Article
    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.
    Insert
    Lyn-PlcR
    Use
    Tags
    N-terminal targeting sequence from Lyn kinase, dd…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181832-plasmid-map-sequence-id-364041

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  33. pcDNA3-Lyn-PlcR

    Plasmid
    #181830
    Purpose
    Genetically encoded FRET-based sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells.
    Depositor
    Jin Zhang
    Article
    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.
    Insert
    Lyn-PlcR
    Use
    Tags
    ECFP, EYFP, and N-terminal targeting sequence fro…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181830-plasmid-map-sequence-id-364215

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  37. pcDNA3-Lyn-μDpTT

    Plasmid
    #182321
    Purpose
    Plasma membrane-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    Lyn-μDronpa-TagRFP-T
    Use
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Available since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182321-plasmid-map-sequence-id-362887

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  46. pcDNA3-Lyn-AktAR

    Plasmid
    #123356
    Purpose
    FRET-based biosensor for monitoring Akt activity in lipid raft plasma membrane regions.
    Depositor
    Jin Zhang
    Article
    Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.
    Insert
    Lyn-AktAR
    Use
    Tags
    Cerulean, N-terminal targeting sequence from Lyn …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    123356-plasmid-map-sequence-id-307195

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  47. Lyn-FLINC-EKAR1

    Plasmid
    #87707
    Purpose
    Fluorescent biosensor for super-resolution imaging of ERK activity; targeted to plasma membrane.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    Lyn-FLINC-EKAR1
    Use
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87707-plasmid-map-sequence-id-171091

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Showing: 1 - 50 of 1251 results