We narrowed to 8,743 results for: ef1

1. pKDR-EF1a.11

   Plasmid

   #183574

   Purpose

   One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Attenuator Sequence 11
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKDR-EF1a.12

   Plasmid

   #183575

   Purpose

   One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Attenuator Sequence 12
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKDR-EF1a.5

   Plasmid

   #183572

   Purpose

   One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Attenuator Sequence 5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKDR-EF1a.3

   Plasmid

   #183570

   Purpose

   One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Attenuator Sequence 3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKDR-EF1a.1

   Plasmid

   #183568

   Purpose

   One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

   Depositor

   Erik Dent

   Article

   Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

   Insert

   Attenuator Sequence 1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTEF1-UDH

   Plasmid

   #166679

   Purpose

   Yeast integrative plasmid for expressing P. syringae uronate dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic marker (K. lactis LEU2).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Insert

   UDH
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET15-EF1n2_mFAP2b

   Plasmid

   #159471

   Purpose

   Encodes for EF1n2_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

   Depositor

   David Baker

   Insert

   EF1n2_mFAP2b
   

   Use

   Tags

   6xHis-thrombin-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET15-EF1n3_mFAP2b

   Plasmid

   #159472

   Purpose

   Encodes for EF1n3_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

   Depositor

   David Baker

   Insert

   EF1n3_mFAP2b
   

   Use

   Tags

   6xHis-thrombin-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET15-EF1n_mFAP2b

   Plasmid

   #159473

   Purpose

   Encodes for EF1n_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

   Depositor

   David Baker

   Insert

   EF1n_mFAP2b
   

   Use

   Tags

   6xHis-thrombin-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAS1_4xh7SKPylT_EF1_SMIM38*_Intein_DD

    Plasmid

    #162814

    Purpose

    STELLA plasmid for amber suppression of C-terminally labelled SMIM38

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    SMIM38(C-TAG)-Intein-DD
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    SMIM38(Ochre52,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAS1_4x7SKPylT_EF1_Ub-*CoV2_M

    Plasmid

    #162815

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_M

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_M
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_M(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAS1_4x7SKPylT_EF1_Ub-*CoV2_9b

    Plasmid

    #162816

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_9b

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_9b
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_9b(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF6

    Plasmid

    #162817

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF6

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF6
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF6(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF7b

    Plasmid

    #162818

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF7b

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF7b
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF7b(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF10

    Plasmid

    #162819

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF10

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF10
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF10(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF9c/14

    Plasmid

    #162820

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF9c/14

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF9c/14
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF9c/14(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b_alt

    Plasmid

    #162821

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b_alt

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)CoV2_ORF3b_alt
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    CoV2_ORF3b_alt(Met1,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAS1_4x7SKPylT_EF1_Ub-*SMIM38

    Plasmid

    #162808

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled SMIM38

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)SMIM38
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    SMIM38 (Met1, Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAS_4x7SKPylT_EF1_Ub-*PIGBOS

    Plasmid

    #162809

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled PIGBOS

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)PIGBOS
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    PIGBOS (Met1, Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHH0103_EEF1A2_GTP-EFTU-D2

    Plasmid

    #109919

    Purpose

    Protein expression and purification of EEF1A2_GTP-EFTU-D2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    EEF1A2_GTP-EFTU-D2 (EEF1A2, EEF1A1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pENTR-L5-EF1_-R3

    Plasmid

    #141037

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    EF1_ promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEF1a-T2-GpNLuc

    Plasmid

    #135949

    Purpose

    Expresses the GpNLuc LumiFluor targeted to the mitochondira in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    GpNLuc LumiFluor
    

    Use

    Tags

    T2 mitochondrial localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEF1a-T2-OgNLuc

    Plasmid

    #135951

    Purpose

    Expresses the OgNLuc LumiFluor targeted to the mitochondira in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    OgNLuc LumiFluor
    

    Use

    Tags

    T2 mitochondrial localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Ad-EEF1D-S133E

    Plasmid

    #131577

    Purpose

    Expresses EEF1D with S133E mutation in mammalian cells

    Depositor

    Haiyan Xu

    Insert

    Eukaryotic translation elongation factor 1 delta (EEF1D Human)
    

    Use

    Adenoviral

    Tags

    No

    Expression

    Mutation

    changed Serine 133 to Glutamic acid

    Promoter

    CMV

    Available since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pIAGO-AceF1

    Plasmid

    #114461

    Purpose

    Expression of late polyene biosynthetic genes in streptomycetes

    Depositor

    Patrick Caffrey

    Article

    Stephens et al Biosci Biotechnol Biochem. 2013;77(4):88

    Insert

    AceDI + AceDII + AceN + AceM
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ermE

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. EF1a_MYC Glu39Ala_P2A_Hygro_Barcode

    Plasmid

    #120512

    Purpose

    Barcoded lentiviral vector to express MYC Glu39Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC Glu39Ala (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Point mutation changing Glutamic Acid to Alanine …

    Promoter

    EF1a

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. YCe2741 HC_Kan_EF1ap_p18

    Plasmid

    #100698

    Purpose

    part designed to occupy position 18 of EMMA. Functional category: Constitutional promoter

    Depositor

    Yizhi Cai

    Article

    Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

    Insert

    EF1a promoter (EEF1A1 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEF1a-mRor2Y641F

    Plasmid

    #22635

    Depositor

    Roel Nusse

    Article

    Mikels et al J Biol Chem. 2009 Aug 31. ():.

    Insert

    Ror2 Y641F (Ror2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed Tyrosine 641 to Phenylalanine

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

29. pEF1a-mRor2Y645F

    Plasmid

    #22636

    Depositor

    Roel Nusse

    Article

    Mikels et al J Biol Chem. 2009 Aug 31. ():.

    Insert

    Ror2 Y645F (Ror2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed Tyrosine 645 to Phenylalanine

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

30. pEF1a-mRor2Y646F

    Plasmid

    #22637

    Depositor

    Roel Nusse

    Article

    Mikels et al J Biol Chem. 2009 Aug 31. ():.

    Insert

    Ror2 Y646F (Ror2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed Tyrosine 646 to Phenylalanine

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

31. LV_EF1a_SV40_Large_T_Antigen-P2A-Hygro_Barcode

    Plasmid

    #170255

    Purpose

    Barcoded lentiviral vector to express SV40 Large T Antigen in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    SV40 Large T Antigen
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHR_EF1a_flagLaM4-Notch-Gal4VP64

    Plasmid

    #216669

    Purpose

    Expresses anti-mCherry synNotch Gal4VP64

    Depositor

    Leonardo Morsut

    Insert

    anti-mCherry synNotch Gal4VP64
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLenti_Ef1a:PE2-P2A-GFP

    Plasmid

    #184445

    Purpose

    Single EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

    Depositor

    Bobby Koeleman

    Article

    Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Ef1A

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pT3-EF1a-YapS127A

    Plasmid

    #46049

    Depositor

    Xin Chen

    Article

    Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    S127A

    Promoter

    EF1a

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

35. pT3-EF1a-NICD1

    Plasmid

    #46047

    Depositor

    Xin Chen

    Article

    Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

    Insert

    Notch1 (C-term) (Notch1 Mouse)
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Cytoplasmic domain initiating at amino acid 1753

    Promoter

    EF1a

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pT3EF1aH-myr-Akt

    Plasmid

    #179909

    Purpose

    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.

    Depositor

    Xin Chen

    Article

    Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Tags

    HA and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. EF1a-dCasRx-RBM25

    Plasmid

    #221001

    Purpose

    Transient expression of dCasRx-RBM25. EF1a promoter.

    Depositor

    Mikko Taipale, Benjamin Blencowe

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    dCasRx-RBM25
    

    Use

    CRISPR

    Tags

    NLS and NLS-HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PL-SIN-EF1α-EGFP

    Plasmid

    #21320

    Purpose

    Control Lentiviral vector with strong EF1a promoter, expresses EGFP

    Depositor

    James Ellis

    Article

    Hotta et al Nat Methods. 2009 May . 6(5):370-6.

    Insert

    Enhanced Green Fluorescence Protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #113341

    Purpose

    Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    VP64-dCas9-VP64-T2A-MS2-p65-HSF1
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV-EF1a-Cre

    Plasmid

    #55636

    Purpose

    Expresses Cre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde and AAV11

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge