173,051 results
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Plasmid#187243PurposeLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expressionDepositorInsertLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
UseLentiviralAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNJP
Plasmid#115494PurposeExpression vector for NLS-iRFP-NLS, JNK KTR-mCherry, and mKO-MK2 in mammalian cellsDepositorTagsiRFP, mCherry, and mKOExpressionMammalianMutationmCherry S227F, mCherry G229R, mKO V211GPromoterCAGAvailable SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC-ZEAXipi
Plasmid#53287PurposeContains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coliDepositorInsertcrtE, idi, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorPromoterendogenous promotersAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_OCT4
Plasmid#26816DepositorInsert5'UTR-OCT4-3'UTR (POU5F1 Human)
ExpressionMammalianAvailable SinceNov. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHAGE-AKT1
Plasmid#116710PurposeLentiviral expression of AKT1DepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3,4,5)P3 Probe
Plasmid#211514PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteriaDepositorInsertBTK1-PH
Tags6xhis-SNAPExpressionBacterialAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC-CANTHipi
Plasmid#53301PurposeContains crtE, idi, crtI, crtY, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and a crtO cDNA of Haematococcus pluvialis fused to a Trc promoter. Produces canthaxanthin in E. coli.DepositorInsertcrtO
UseLow copy number bacterial cloning vectorTags6HisPromoterTrcAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSUB11
Plasmid#169699PurposeTemplate plasmid for generating C-terminal 3xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert3xFLAG tag adjacent to FRT-flanked aph (KanR) gene
Tags3xFLAG tagExpressionBacterialAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO V5 HSPA1A
Plasmid#19510DepositorAvailable SinceOct. 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
PBX2-pSP64
Plasmid#21032DepositorInsertPBX2 (PBX2 Human)
UseIn vitro tntAvailable SinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTU2-AmyE-Pveg-eGFP
Plasmid#112792PurposeExpresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformationDepositorArticleInsertPveg-eGFP
ExpressionBacterialAvailable SinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hM3D(Gq)-mCherry
Plasmid#50476PurposeGq-coupled hM3D DREADD fused with mCherry under the control of CaMKIIa promoterDepositorHas ServiceAAV1, AAV2, AAV5, AAV8, and AAV9InserthM3D(Gq)-mCherry (CHRM3 Human)
UseAAVTagsmCherryMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable SinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA
Plasmid#37120PurposeDO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, expresses EGFP in Cre positive cellsDepositorInsertTdTomato-EGFP
UseAAV and Cre/Lox; Cre-switchPromoterEF1aAvailable SinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
S48I48 ELP
Plasmid#68394PurposeElastin-like polypeptide (VPGxG) with 48 repeats of serine guest residue followed by 48 repeats of isoleucine guest residueDepositorInsertS48I48 ELP
ExpressionBacterialPromoterT7Available SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBSDONR P1-P4
Plasmid#71744Purposeampicillin resistant derivative of pDONR221 P1-P4 for multisite GATEWAY cloningDepositorTypeEmpty backboneUseGateway entry vector for use in multisite gateway…Available SinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Ubiquitin
Plasmid#187910PurposeMammalian cell expression of EGFP-tagged UbiquitinDepositorAvailable SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSynI-V5-TurboID(N)-GPI
Plasmid#163696PurposeExpresses n-terminal half of TurboID on surface of neuronsDepositorInsertGPI anchored TurboID
UseAAVAvailable SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-TMEM106b-mApple
Plasmid#179385PurposeLentiviral vector for expression of TMEM106b fused mAppleDepositorInsertTMEM106b - mApple
UseLentiviralTagsmAppleExpressionMammalianPromoterEF1Available SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK V5-LUC Puro (w543-1)
Plasmid#19360Purpose3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, PuroDepositorInsertFirefly Luciferase
UseLentiviral and LuciferaseTagsV5ExpressionMammalianAvailable SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-D1ER
Plasmid#36325DepositorInsertD1ER second generation cameleon (calcium sensor) targeted to ER
TagsKDELExpressionMammalianPromoterCMVAvailable SinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBiFCt-2in1-NN
Plasmid#105111Purposeratiometric BiFC analysis in plants (transient transformation)DepositorTypeEmpty backboneTagsnYFP/cYFPExpressionPlantAvailable SinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1
Plasmid#118593PurposeExpresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoterDepositorInsertPuro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)
UseLentiviralExpressionMammalianPromoterEF1Alpha/TRE3GAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-UtrCH
Plasmid#26737DepositorInsertUtrophin
UseReporter for f-actinTagsGFPExpressionMammalianMutationCalponin homology domain of utrophin (aa# 1-261).…PromoterCMV IE94Available SinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_25bpLinker
Plasmid#157788PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_25bp linker
ExpressionBacterialAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pGP-CAG-iGABASnFR2-WPRE-bGH-polyA
Plasmid#218868PurposeMammalian expression of improved GABA sensor (positive change in fluorescence)DepositorInsertiGABASnFR2
ExpressionMammalianMutationS99A F102Y F104Y L178SPromoterCAGAvailable SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hNFATc2
Plasmid#74050Purposeretroviral expression plasmid for human NFATc2/CDepositorInserthuman NFATc2, isoform C (NFATC2 Human)
UseRetroviralTagsno tagExpressionMammalianMutationsilent mutation A1723C in the sequence of NM_1730…PromoterpMSCV-LTRsAvailable SinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_mTagBFP2
Plasmid#113725PurposeExpresses the fluorescent protein mTagBFP2 in a lentiviral backboneDepositorInsertmTagBFP2
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-6xHis-AviTag-ALFAtag-eGFP
Plasmid#195854PurposeBacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.DepositorInsert6xHis-AviTag-ALFAtag-eGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP×2-N1
Plasmid#86775PurposeA mammalian expression vector with double EGFP at C-terminus.DepositorTypeEmpty backboneExpressionMammalianPromoterCMVAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pF146 pSOD1WTAcGFP1
Plasmid#26407DepositorAvailable SinceNov. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
P4 cosmid
Plasmid#196335PurposeP4 cosmid with CRISPR-Cas9 systemDepositorInsertgenes from phage P4, CRISPR-Cas9 system
UseCRISPRExpressionBacterialAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEarleyGate 100RMOA mPing
Plasmid#196137PurposeMeasures mPing transposition in plantsDepositorInsertsPong TPase LA T2A ORF1SC1 ONE
mPing
UseBinaryExpressionPlantMutationTPase NES sequence mutated L418A, L420A; T2A pept…Promoter35S and RPS5aAvailable SinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro
Plasmid#138152PurposeExpresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleusDepositorInserteGFP-MCS
UseLentiviralTags2PEST AND NLSAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
G5-CD40
Plasmid#121166PurposepMP71 vector expressing GFP - P2A - G5 - myc - CD40DepositorInsertG5-CD40
UseRetroviralTagsGFP and mycExpressionMammalianAvailable SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Smad4
Plasmid#80888Purposemammalian expression of Smad4DepositorAvailable SinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2 (GFP)
Plasmid#80924PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorTypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…ExpressionMammalianPromoterTREAvailable SinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRL092 pattB_cryaa:Venus_MCS
Plasmid#213513PurposeattB-containing vector backbone, for use in restriction enzyme- or PCR-based cloningDepositorTypeEmpty backboneUseZebrafish expressionAvailable SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOT_2 - lenti-EFS-tNGFR-2A-puro
Plasmid#181971PurposeExpresses human truncated NGFR linked to puromycin resistance via P2A for lentiviral delivery.DepositorAvailable SinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-CRISPRoffv2.1-IRES-BlastR
Plasmid#207180PurposeLentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a PromoterDepositorInsertCRISPRoffv2.1
UseCRISPR and LentiviralTagsHA and TagBFPExpressionMammalianPromoterEF1aAvailable SinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only