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163,606 results

Showing: 3961 - 4000 of 163606 results

  1. CROPseq-EFS-SpCas9-P2A-blast

    Plasmid
    #106477
    Purpose
    single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available Since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106477-plasmid-map-sequence-id-207983

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  8. TFORF0799

    Plasmid
    #141705
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ATF3 (ATF3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141705-plasmid-map-sequence-id-404521

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  13. INCTbiosyn-p35S(rc)2_4_5-GFP

    Plasmid
    #127520
    Purpose
    Plasmid has an inverted CaMV 35S promoter sequence (reverse complement) flanked by attB and attP attachment sites of integrases 2, 4, and 5. EGFP coding sequence is in the forward orientation.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    CaMV 35S reverse complement promoter sequence flanked by attB/attP Integrase 2, 4 and 5 attachment sites.
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127520-plasmid-map-sequence-id-250826

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  15. pFlp-Km

    Plasmid
    #222334
    Purpose
    Expresses Flp and kanamycin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3
    Depositor
    Philip Poole
    Article
    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10
    Inserts
    sacB (sacB B. subtilis)
    flp (flp Budding Yeast)
    cI (cI )
    Use
    Tags
    Expression
    Mutation
    Promoter
    cl, flp, and sacB
    Available Since
    Aug. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    222334-plasmid-map-sequence-id-442294

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  17. pN251

    Plasmid
    #188471
    Purpose
    Lentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)
    Depositor
    Le Cong
    Article
    Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2
    Insert
    enCas12a-HF (gift from Dr. Keith Joung)
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available Since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    188471-plasmid-map-sequence-id-370973

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  25. ABE7.10-F148A

    Plasmid
    #132946
    Purpose
    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.
    Depositor
    Hui Yang
    Article
    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:
    Insert
    ABE7.10(F148A)
    Use
    CRISPR; Base editor
    Tags
    Expression
    Mammalian
    Mutation
    ABE7.10(F148A)
    Promoter
    Available Since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    132946-plasmid-map-sequence-id-314827

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  27. pcDNA3/td5StayGold(c4)=GianCreg

    Plasmid
    #212022
    Purpose
    For labeling the Golgi apparatus. c4 is the appendage for N-terminal tagging with td5StayGold. ‘GianCreg’ is an abbreviation for giantin C-terminal region. ‘=’ denotes a linker [(GGGGS)3].
    Depositor
    Atsushi Miyawaki
    Article
    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Insert
    GianCreg (GOLGB1 Human)
    Use
    Tags
    td5StayGold(c4)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    212022-plasmid-map-sequence-id-419221

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  30. pEVOL-pAzF[TAG]

    Plasmid
    #164579
    Purpose
    Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons
    Depositor
    Ryan Mehl
    Article
    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2
    Inserts
    Mj pCNFRS[TAG] (aaRS1)
    Mj pCNFRS[TAG] (aaRS2)
    Mj tRNA[TAG]
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    araBAD, glnS, and proK
    Available Since
    May 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    164579-plasmid-map-sequence-id-392918

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  31. TFORF1329

    Plasmid
    #143789
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TFAP2A (TFAP2A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143789-plasmid-map-sequence-id-423575

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  35. TFORF0144

    Plasmid
    #141536
    Purpose
    Lentiviral vector for overexpressing the PHOX2A transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PHOX2A (PHOX2A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141536-plasmid-map-sequence-id-276744

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  40. pExp-sfGFP

    Plasmid
    #112573
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis, Strep II, and sfGFP
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112573-plasmid-map-sequence-id-217227

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Showing: 3961 - 4000 of 163606 results