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We narrowed to 23,989 results for: Sis

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Showing: 4081 - 4120 of 23989 results

  2. pAN19-ccvTIR1-GUS-NOSt

    Plasmid
    #108548
    Purpose
    Cloning vector including C-terminally GUS-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator
    Depositor
    Keiko Torii
    Article
    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d
    Insert
    TRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator (TIR1 Mustard Weed)
    Use
    Cloning vector
    Tags
    GUS
    Mutation
    Changed F79 to G
    Available Since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    108548-plasmid-map-sequence-id-210051

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  3. MSCV-PIG-N3FLAG-TAF12

    Plasmid
    #105593
    Purpose
    retrovirally express mouse TAF12 with 3*FLAG tag at N terminal, GFP marker and puro resistance
    Depositor
    Christopher Vakoc
    Article
    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.
    Insert
    full length TAF12 with silent mutations, making it resistant to TAF12 shRNA#364 (Taf12 Mouse)
    Use
    Retroviral
    Tags
    3*FLAG
    Expression
    Mammalian
    Mutation
    silent mutations to make it resistant to the targ…
    Promoter
    MSCV-LTR
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    105593-plasmid-map-sequence-id-209432

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  4. pMCh-1522

    Plasmid
    #82428
    Purpose
    Plasmid for expression of GST-tagged C-terminal region of Nematostella vectensis GW182 11W11A (aa 1159-1698) in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 CED 11W11A
    Tags
    GST
    Expression
    Mammalian
    Mutation
    amino acids 1-1158 of nvGW182 were deleted and Tr…
    Promoter
    EF1A
    Available Since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82428-plasmid-map-sequence-id-156193

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  5. pMCh-1526

    Plasmid
    #82429
    Purpose
    Plasmid for expression of NHA-tagged C-terminal region of Nematostella vectensis GW182 11W11A (aa 1159-1698) in mammalian cells
    Depositor
    Marina Chekulaeva
    Article
    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7
    Insert
    nvGW182 CED 11W11A
    Tags
    NHA
    Expression
    Mammalian
    Mutation
    amino acids 1-1158 of nvGW182 were deleted and Tr…
    Promoter
    CMV
    Available Since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    82429-plasmid-map-sequence-id-156191

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  8. pMVP-SEC24C

    Plasmid
    #231559
    Purpose
    pMVP expression vector for human SEC24C (wild type, closed, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)
    Depositor
    Rizwan Haq
    Article
    Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e
    Insert
    SEC24C (SEC24C Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    silent PAM mutations (G67 (GGG>GGA) and A115 (…
    Promoter
    CMV
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231559-plasmid-map-sequence-id-459740

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  13. pICE-RNaseHI-D10R-E48R-NLS-mCherry

    Plasmid
    #60367
    Purpose
    Plasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Depositor
    Sébastien Britton, Patrick Calsou
    Article
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Insert
    RNase HI-D10R-E48R
    Tags
    NLS from SV40 T antigen and mCherry
    Expression
    Mammalian
    Mutation
    D10R+E48R; catalyticaly inactive
    Promoter
    CMV-tet
    Available Since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60367-plasmid-map-sequence-id-456413

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  14. pRL_GI_PCB28

    Plasmid
    #98745
    Purpose
    For phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919
    Inserts
    heme oxygenase 1 (TED4 Mustard Weed)
    Phycocyanobilin:ferredoxin oxidoreductase
    Use
    Synthetic Biology
    Expression
    Yeast
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    ADH1m and PGK1
    Available Since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98745-plasmid-map-sequence-id-189854

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  23. pCAGGS-PHFF

    Plasmid
    #100280
    Purpose
    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Insert
    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    Tags
    FLAG, T7 and Mitochondria-targeting sequence (MTS…
    Expression
    Mammalian
    Mutation
    All genes are codon-optimized for expression in h…
    Promoter
    CAG promoter
    Available Since
    Sept. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    100280-plasmid-map-sequence-id-194335

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  27. pNOC_dCas9_sgRNA NC

    Plasmid
    #176259
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176259-plasmid-map-sequence-id-346581

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  29. pICE-HA-CtIP-siR-N289A-H290A

    Plasmid
    #82032
    Purpose
    Plasmid for constitutive or doxy-inducible expression of N289A+H290A mutant of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Sébastien Britton, Patrick Calsou
    Article
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Insert
    CtIP (RBBP8 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Endonuclease-dead mutant: N289A+H290A CtIP; Mutat…
    Promoter
    CMV-tet
    Available Since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82032-plasmid-map-sequence-id-155382

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  31. pICE-HA-CtIP-siR-S664A-S679A-S745A

    Plasmid
    #82031
    Purpose
    Plasmid for constitutive or doxy-inducible expression of S664A+S679A+S745A human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Sébastien Britton, Patrick Calsou
    Article
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Insert
    CtIP (RBBP8 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Non phosphorylatable mutations on 3 of the main A…
    Promoter
    CMV-tet
    Available Since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82031-plasmid-map-sequence-id-155380

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  32. sgSf3b1(T1)

    Plasmid
    #90424
    Purpose
    induces dsDNA break within mouse Sf3b1 gene for subsequent homology-directed recombination and coding sequence mutagenesis at codon 700
    Depositor
    Alex Minella
    Article
    Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519
    Insert
    mus Sf3b1 gRNA (Sf3b1 Mouse)
    Use
    CRISPR; Guiderna encoding for gene editing
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    90424-plasmid-map-sequence-id-190772

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  33. pNOC_dfnCas12a_crRNA NC

    Plasmid
    #176256
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and spacer sequence is replaced by the type IIS restriction site for endonuclease BaeI that can be
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176256-plasmid-map-sequence-id-346577

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  34. pNOC_hfnCas12a-Nlux_crRNA NR1

    Plasmid
    #176244
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Promoter
    Ribosomal subunit
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176244-plasmid-map-sequence-id-346573

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  39. pWZLblasti-HA-MEK1-MEK5DD

    Plasmid
    #53177
    Purpose
    Expresses HA tagged chimeric human MEK1 (nucleotides 1–201)-MEK5 (498–1344 with S311DT315D) from blasticidin resistance retroviral vector
    Depositor
    Christopher Counter
    Article
    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1
    Insert
    MEK1 (1-201)-MEK5 (498–1344) fusion (MAP2K1 Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    MEK1 (1-201) MEK5(498–1344 with S311D/T315D)
    Available Since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only

  40. pENTR-BS-AtMIR390a-A18G-B/c

    Plasmid
    #246715
    Purpose
    Entry plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.
    Depositor
    Alberto Carbonell
    Article
    Llorens-Gamez et al Plant J. 2026 Jan;125(1):e70665. doi: 10
    Insert
    Basal stem of Arabidopsis MIR390a precursor with A18G mutation and a chloramphenicol-ccdB cassette flanked by 2 BsaI sites (MIR390a Mustard Weed)
    Use
    Gateway-compatible entry vector
    Available Since
    Jan. 14, 2026
    Availability
    Academic Institutions and Nonprofits only
    246715-plasmid-map-sequence-id-494277

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Showing: 4081 - 4120 of 23989 results