-
Plasmid#188489PurposeExpresses FLAG tagged SpCas9DepositorInsertCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG-Cas9
Plasmid#89995PurposeExpresses high levels of WT Cas9 for genome editingDepositorInserthCas9
UseTags3xFlag, SV40 NLS, and T2A peptideExpressionMammalianMutationPromoterCAGAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFD116
Plasmid#124769PurposepFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vectorDepositorInsertsPtet
dcas9
PpflB sgRNA BsaI
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS415_pGal-nCas9(D10A)
Plasmid#79618PurposeExpresses nCas9(D10A) in yeast cellsDepositorInsertSpCas9
UseTagsExpressionYeastMutationD10A for nickase Cas9PromoterpGal1Available sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIVT-dCas9-EGFP
Plasmid#174442Purposein vitro transcription template for dCas9-EGFPDepositorInsertCas9 Endonuclease Dead
UseIn vitro transcription templateTagsEGFPExpressionMutationPromoterT7 SP6Available sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMJ841
Plasmid#39318DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationAspartate 10 to Alanine (D10A) and Histidine 840 …PromoterT7Available sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHSB04X
Plasmid#117258PurposeGenome editing for Lactobacillus brevis ATCC367DepositorInsertCas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019
UseOtherTagsExpressionMutationPromoterAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMBP-SpyCatcher
Plasmid#79053PurposeExpresses maltose binding protein (MBP) SpyCatcher fusionDepositorInsertMBP-SpyCatcher
UseTags6xHis and Maltose binding proteinExpressionBacterialMutationDerived from pDEST14-SpyCatcher (Plasmid #35044)…PromoterT7Available sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMLM3705
Plasmid#47754PurposeExpresses mammalian cell codon-optimized dCas9-VP64DepositorInsertcodon optimized dCas9-VP64
UseCRISPRTags3x FLAG, NLS, and VP64ExpressionMammalianMutationD10A and H840A mutations in Cas9 (catalytically i…PromoterCMVAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBBK21 pCAS-Tyr-[gRNA: 4=XII-5] (SplitHygR, AmpR)
Plasmid#179005PurposeSp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK20 pCAS-Tyr-[gRNA: 3=XII-2] (SplitHygR, AmpR)
Plasmid#179004PurposeSp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGM4723:TpCC_Urease
Plasmid#85982PurposeGolden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAsDepositorInsertsCas9
Urease-targeting gRNA 1
Urease-targeting gRNA 2
UseCRISPR; Diatom (t. pseudonana)TagsNLS and YFPExpressionMutationPromoterFCP and U6Available sinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-1
Plasmid#61736PurposeStreptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNADepositorInsertssSpCas9
tracrRNA
crRNA cassette
UseCRISPRTagsExpressionBacterialMutationBbsI-flanked lacZ cassette inserted in place of s…Promotergapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsIAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBBK03 pCAS-Tyr-[gRNA: BsaI GFP dropout] (HygR,AmpR)
Plasmid#178987PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pMSCVneo
Plasmid#134982PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR and RetroviralTags3xFLAG tag and NLSExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterLTRAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pMSCVpuro
Plasmid#134983PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR and RetroviralTags3xFLAG tag and NLSExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterLTRAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBBK04 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitHygR,AmpR)
Plasmid#178988PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCas9-beta
Plasmid#169240PurposeCas9 and beta expression plasmid for performing gene knockouts via oligo recombineeringDepositorInsertscas9
beta
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Fragment 1 Null
Plasmid#198729PurposeGateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA.DepositorInsertgRNA expression cassette in pENTR4
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJH001
Plasmid#67641PurposeHigh copy Cas9 expression vector with LEU2 marker for yeast transformationDepositorInsertsCas9
Empty
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIVT-dCas9-mCherry
Plasmid#174443Purposein vitro transcription template for dCas9-mCherryDepositorInsertCas9 Endonuclease Dead
UseIn vitro transcription templateTagsmCherryExpressionMutationPromoterT7 SP6Available sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBBK95 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (HygR)
Plasmid#179079PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ubiq-Cas9.874W
Plasmid#112686PurposeExpress hSpCas9 under Ubiquitin-63E promoterDepositorInserthSpCas9
UseTags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoterUbiquitin-63E promoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
vas-Cas9.874Z
Plasmid#112687PurposeExpress hSpCas9 under vasa promoterDepositorInserthSpCas9
UseTags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromotervasaAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher EQ
Plasmid#35045DepositorInsertSpyCatcher EQ
UseTagsHis6ExpressionBacterialMutationDeletion of C-terminal till I113, and point mutat…PromoterAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRS415_pGal-nCas9(H840A)
Plasmid#79616PurposeExpresses nCas9(H840A) in yeast cellsDepositorInsertSpCas9
UseTagsExpressionYeastMutationH840A for nickase Cas9PromoterpGal1Available sinceSept. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-3'UTR-Tetra-com-vector
Plasmid#132553PurposeExpresses spCas9 and sgRNA scaffold with com aptamer replacing the TetraloopDepositorInsertsCas9
sgRNA scaffold with com replacing the Tetraloop
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
nos-Cas9.874Z1
Plasmid#112685PurposeExpress hSpCas9 under nanos promoterDepositorInserthSpCas9
UseTags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoternanosAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBBK01 pCAS-Tyr-[gRNA: BsaI GFP dropout] (KanR, AmpR)
Plasmid#178985PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK05 pCAS-Tyr-[gRNA: BsaI GFP dropout] (NatR,AmpR)
Plasmid#178989PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Nourseothricin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJT25_GalL_BE3
Plasmid#143736PurposeExpresses BE3 in yeast cellsDepositorInsertBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Cas9-tdTomato
Plasmid#141013PurposeMutisite Gateway mediated vector constructionDepositorInsertCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_3
Plasmid#60575Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_3
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_10
Plasmid#60581Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_10
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_4
Plasmid#60576Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_4
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCF730
Plasmid#121668PurposeEF1a-ProCas9-Flavi-P2A-PuroDepositorInsertCas9(C)
UseLentiviralTagsExpressionMutationPromoterEF-1a core promoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Sox1/2/3-2373/+3>nls::Cas9::nls
Plasmid#59989PurposeSox1/2/3 (SoxB1) driver driving nls::Cas9::nlsDepositorInsertnls::Cas9::nls
UseCRISPRTagsExpressionMutationReverted mutations from dCas9 to wiltdtypePromoterCiinte.Sox1/2/3 (SoxB1) -2373/+3Available sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJT42_GalL_BE-PAPAPA
Plasmid#145035PurposeExpresses BE-PAPAPA in yeast cellsDepositorInsertBE-PAPAP
UseCRISPRTagsExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCas9-Nours
Plasmid#107733PurposeEscherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiaeDepositorInsertCas9
UseCRISPRTagsSV40 NLSExpressionYeastMutationHuman optimizedPromoterTEF1Available sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS315e_pGal-dCas9-SH3
Plasmid#79613PurposeExpresses dCas9-SH3 in yeast cellsDepositorInsertSpCas9
UseTagsSH3 domainExpressionYeastMutationD10A and H840A for nuclease deficient Cas9PromoterpGal1Available sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only