4,133 results

1. pF141 pAcGFP1 SOD1WT

   Plasmid

   #26402

   Depositor

   Elizabeth Fisher

   Article

   Stevens et al PLoS One. 2010 . 5(3):e9541.

   Insert

   SOD1 (SOD1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRK7-FLAG-TSC1

   Plasmid

   #8995

   Depositor

   John Blenis

   Article

   Tee et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

   Insert

   TSC1 (TSC1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCI neo-hEST2

   Plasmid

   #1781

   Depositor

   Bob Weinberg

   Article

   Meyerson et al Cell 1997 Aug 22;90(4):785-95.

   Insert

   hTERT (TERT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAnc80L65

   Plasmid

   #68837

   Purpose

   Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for details

   Depositor

   Luk Vandenberghe

   Article

   Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1

   Insert

   Ancestral AAV Capsid Anc80L65
   

   Use

   AAV and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Rep2

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-myc3-HDM2

   Plasmid

   #20935

   Depositor

   Yue Xiong

   Article

   Zhang et al Mol Cell Biol. 2003 Dec . 23(23):8902-12

   Insert

   Mdm2 p53 binding protein homolog (MDM2 Human)
   

   Use

   Tags

   myc3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 7, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Pyronic/pcDNA3.1(-)

   Plasmid

   #51308

   Purpose

   Pyronic, FRET sensor that allow quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resolution.

   Depositor

   Luis Felipe Barros

   Article

   San Martin et al PLoS One. 2014 Jan 21;9(1):e85780. doi:

   Insert

   Pyronic
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHYRS52

   Plasmid

   #31122

   Depositor

   Hideo Iwai

   Article

   Muona et al Chembiochem. 2008 Dec 15. 9(18):2958-61.

   Insert

   Ulp1 (ULP1 Budding Yeast)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Ulp1(403-621)

   Promoter

   Available since

   Sept. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. tetO-MEF2C

   Plasmid

   #46031

   Depositor

   John Gearhart

   Article

   Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

   Insert

   MEF2C (MEF2C Human)
   

   Use

   Lentiviral

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   July 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pENTR1A-Huwe1

   Plasmid

   #37431

   Depositor

   Jean Cook

   Insert

   Huwe1 (HUWE1 Human)
   

   Use

   Gateway entry

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCFJ150 - pDESTttTi5605[R4-R3]

    Plasmid

    #19329

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    pDESTttTi5605[R4-R3]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHR-CMV-nlsCRE

    Plasmid

    #12265

    Depositor

    Didier Trono

    Article

    Cudre-Mauroux et al Hum Gene Ther. 2003 Nov 1. 14(16):1525-3

    Insert

    Cre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCruzHA SIRT1

    Plasmid

    #10962

    Depositor

    Toren Finkel

    Article

    Nemoto et al J Biol Chem. 2005 Apr 22. 280(16):16456-

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET15bpATnp

    Plasmid

    #121137

    Purpose

    This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

    Depositor

    Keji Zhao

    Article

    Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

    Insert

    Protein A-Tnp
    

    Use

    Tags

    his

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HA PKB T308D S473D pcDNA3

    Plasmid

    #14751

    Depositor

    Jim Woodgett

    Article

    Scheid et al Mol Cell Biol. 2002 Sep . 22(17):6247-60

    Insert

    PKB DD (AKT1 Bovine)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T308D S473D. Constitutively active mutant.

    Promoter

    Available since

    April 12, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pcDNA-D1ER

    Plasmid

    #36325

    Depositor

    Roger Tsien, Amy Palmer

    Article

    Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

    Insert

    D1ER second generation cameleon (calcium sensor) targeted to ER
    

    Use

    Tags

    KDEL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MSCV PIG (Puro IRES GFP)

    Plasmid

    #18751

    Depositor

    Scott Lowe

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSLQ1651-sgRNA(F+E)-sgGal4

    Plasmid

    #100549

    Purpose

    sgGal4-expressing vector modified from Addgene plasmid #51024

    Depositor

    Jian Xu

    Article

    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

    Insert

    sgGal4
    

    Use

    CRISPR

    Tags

    sgRNA

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)

    Plasmid

    #71707

    Purpose

    A human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmid

    Depositor

    Giulio Draetta

    Article

    Gutschner et al Cell Rep. 2016 Feb 3. pii: S2211-1247(16

    Insert

    humanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)
    

    Use

    CRISPR

    Tags

    3xFLAG and hGEM(1/110)

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EF.hICN1.Ubc.GFP

    Plasmid

    #17626

    Depositor

    Linzhao Cheng

    Article

    Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

    Insert

    ICN1 (NOTCH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSET-mSA-EGFP

    Plasmid

    #39862

    Depositor

    Sheldon Park

    Article

    Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

    Insert

    monomeric streptavidin fused to EGFP
    

    Use

    Tags

    6xHis, EGFP, and FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. FR_HNF4A2

    Plasmid

    #31100

    Depositor

    Gerhart Ryffel

    Article

    Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    splice variant 2

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSicoR

    Plasmid

    #11579

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF shRNA expression.

    Depositor

    Tyler Jacks

    Article

    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. DsRed2-N1

    Plasmid

    #54493

    Purpose

    Localization: N1 Cloning Vector, Excitation: 558, Emission: 583

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    DsRed2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pLB

    Plasmid

    #11619

    Purpose

    pLB is a modification of pLL3.7 (Addgene#11795). Genetic elements known to prevent epigenetic silencing were added.

    Depositor

    Stephan Kissler

    Article

    Kissler et al Nat Genet. 2006 Apr . 38(4):479-83.

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCW57.1-FOXM1b

    Plasmid

    #68811

    Purpose

    Doxycycline inducible lentiviral vector for FOXM1b expression in mammalian cells

    Depositor

    Adam Karpf

    Article

    Barger et al Oncotarget. 2015 Jul 16.

    Insert

    FOXM1b (FOXM1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-cdk5GFP

    Plasmid

    #1346

    Depositor

    Li-Huei Tsai

    Insert

    CDK5 (CDK5 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mCherry-BP1-2 pLPC-Puro

    Plasmid

    #19835

    Depositor

    Titia de Lange

    Article

    Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

    Insert

    Fragment of p53-Binding Protein 1 (TP53BP1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mutation

    Fragment of human 53BP1 aa 1220 - 1711

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. YAP1 (S6A) - V5 in pLX304

    Plasmid

    #42562

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S61A, S109A, S127A, S128A, S131A, S163A, S164A, S…

    Promoter

    CMV

    Available since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCGN-ELK-1

    Plasmid

    #27156

    Purpose

    For mammalian expression of human Elk-1 with an HA tag

    Depositor

    Ron Prywes

    Article

    Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

    Insert

    ELK1 (ELK1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. IL6-GFP

    Plasmid

    #28088

    Depositor

    Jennifer Stow

    Article

    Manderson et al J Cell Biol. 2007 Jul 2. 178(1):57-69.

    Insert

    IL6 (Il6 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge