We narrowed to 41,926 results for: tro

Showing: 4441 - 4480 of 41926 results

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pROF021

Plasmid

#155326

Purpose

Expression of PULSE-controlled FLuc

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

(etr)8-(C120)5-PhCMVmin-FLuc-TSV40

Use

Luciferase and Synthetic Biology; Eukaryotic expr…

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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Lyn11-CIBN-GFP

Plasmid

#79572

Purpose

expression of plasma membrane signal from Lyn11 fused to CIBN and tagged with GFP

Depositor

Pietro De Camilli, Olof Idevall-Hagren

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

Lyn11

Tags

CIBN (CRY2-binding domain) and EGFP

Expression

Mammalian

Mutation

G8R (please see depositor comments below)

Promoter

CMV

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
Mammalian expression: Z-lock cofilin dark state mutant

Plasmid

#141136

Purpose

a control for optogenetic cofilin analog

Depositor

Klaus Hahn

Article

Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

Insert

Zdk2, Cofilin, LOV2

Expression

Mammalian

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-BRX-eGFP-NLS

Plasmid

#217534

Purpose

AAV vector to drive the expression of eGFP under the control of the 4xBRE regulatory element of SMAD1 and miniXon splicing casette transcription factor in vivo

Depositor

Peter Scheiffele

Article

Okur et al bioRxiv 2023

Insert

BMP reporter element and miniXon casette (Smad1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

BMP Reporter Element

Available Since

April 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-FRT-EF1a-TVA-2A-N2cG

Plasmid

#172361

Purpose

Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from Flp-expressing neurons

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

TVA + B19-N2cG

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.AX

Plasmid

#195218

Purpose

AAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)

Depositor

Viviana Gradinaru

Article

Jang et al Nat Biotechnol. 2023 Jan 26. doi: 10.103

Insert

Synthetic construct isolate AAV-PHP.AX VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-hsFlt3L

Plasmid

#25074

Depositor

Maria Castro

Article

Curtin et al J Immunol. 2006 Mar 15. 176(6):3566-77.

Insert

human soluble FMS-like tyrosine kinase 3 ligand (FLT3LG Human)

Expression

Bacterial

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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Lenti-eCas9-sgNT

Plasmid

#140238

Purpose

Lentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNA

Depositor

Urs Greber

Article

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Insert

non-targeting sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAH34 (Phsp-16.41::cGAL-N::let-858 3'UTR)

Plasmid

#107133

Purpose

cGAL-N driver under the control of C. elegans hsp-16.41 promoter

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::cGAL(DBD)::gp41-1-N-intein

Expression

Worm

Promoter

C. elegans hsp-16.41 promoter

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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miRFP670-TRF1

Plasmid

#227388

Purpose

Label telomere, no adhesion

Depositor

Clifford Brangwynne

Article

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

Telomeric miRFP (TERF1 Human)

Use

Lentiviral

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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slc1a3b:myrGCaMP6s-P2A-H2AmCherry

Plasmid

#170209

Purpose

Drives myrGCaMP6s-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGCaMP6s calcium indicator expressed in the membrane and mCherry labels the nucleus

Depositor

Kelly Monk

Article

Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

Insert

slc1a3b (slc1a3b Zebrafish)

Use

Zebrafish

Tags

myrGCaMP6s-P2A-H2AmCherry

Promoter

slc1a3b

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pT2/CMV-Luc/PGK-SB100X

Plasmid

#192869

Purpose

Carries luciferase under CMV promoter flanked with IR/DR elements. Also carries SB100X under PGK promoter outside of the IR/DR elements.

Depositor

Maria Castro, David Largaespada

Article

Calinescu et al J Vis Exp. 2015 Feb 22;(96). doi: 10.379

Inserts

luc
SB100X

Expression

Mammalian

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

Plasmid

#46168

Purpose

Codon optimized Cas9_SV40 NLS with intron

Depositor

John Calarco

Article

Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

Insert

codon optimized Cas9_SV40 NLS with intron

Use

CRISPR

Mutation

intron inserted at position 3113-3163, and SV40 N…

Promoter

eef-1A.1 (eft-3)

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-Ef1a-TVA-2A-N2cG

Plasmid

#172360

Purpose

Targeting envA-pseodotyped RVdG vectors for specific retrograde labeling specifically from cre-expressing neurons

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

TVA + B19-N2cG

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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RabV CVS-N2c(deltaG)-mCherry-P2A-Cre

Plasmid

#73472

Purpose

Expresses mCherry and Cre for monosynaptic circuit mapping and genetic manipulation

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

mCherry-P2A-Cre

Use

Neurotropic virus

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLHCX-gpASNase1

Plasmid

#121526

Purpose

Expresses gpASNase1 in mammalian cells.

Depositor

Lucas Sullivan, Matthew Vander Heiden

Article

Sullivan et al Nat Cell Biol. 2018 Jul;20(7):782-788. d

Insert

gpASNase1

Use

Retroviral

Tags

FLAG

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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Linear-GFP

Plasmid

#226258

Purpose

Production of linear RNA via IVT

Depositor

Prashant Mali

Article

Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

Insert

EGFP

Use

In vitro transcription

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGATOR-DTA

Plasmid

#64103

Purpose

Tet inducible, Gateway adapted ROSA26 targeting Vector with DTA

Depositor

Bill Skarnes

Article

Melidoni et al Proc Natl Acad Sci U S A. 2013 Oct 29;11

Insert

rTTA (Gt(ROSA)26Sor Synthetic)

Use

Mouse Targeting; Tet inducible

Expression

Mammalian

Promoter

ROSA26 endogenous

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pMK364 (CMV-OsTIR1-loxP-PURO-loxP)

Plasmid

#121184

Purpose

OsTIR1 introduction

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

CMV-OsTIR1-loxP-PURO-loxP

Use

CRISPR

Mutation

OsTIR1 codon optimized for human cells

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMRS-XylS-dCas9

Plasmid

#220196

Purpose

mini-Tn7 dCas9 expression vector - XylS/pM promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9

Use

CRISPR

Expression

Bacterial

Mutation

Pseudomonas aeruginosa codon optimized

Promoter

XylS/pM

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-CRY2-5ptaseOCRL

Plasmid

#66836

Purpose

Expression of inositol 5-phosphatase domain of OCRL. Binds to CIBN upon blue light illumination.

Depositor

Pietro De Camilli

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

5ptaseOCRL (OCRL Human)

Tags

mCherry-CRY2

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pMRS-LacI-GFP

Plasmid

#220198

Purpose

mini-Tn7 GFP expression vector - LacI/pLac promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

monomeric superfolder green fluorescent protein

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

LacI/pLac

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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VinTS-tCRMod-GGSGGS9

Plasmid

#111765

Purpose

Expresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 module

Depositor

Brenton Hoffman

Article

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMRS-BG42-GFP

Plasmid

#220202

Purpose

mini-Tn7 GFP expression vector - pBG42 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

monomeric superfolder green fluorescent protein

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG42

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-P2X2-YFP

Plasmid

#22400

Depositor

Henry Lester

Article

Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

Insert

ATP-gated P2X channel 2 (P2rx2 Rat)

Tags

YFP

Expression

Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pBetaActin-mNeonGreen-CAMSAP3

Plasmid

#191322

Purpose

Expresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flow

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

mNeonGreen-CAMSAP3 (Camsap3 Mouse)

Expression

Mammalian

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Split PS Intein tTA

Plasmid

#242028

Purpose

Blue-light-controlled nuclear translocation of split tTA transactivator from the cytoplasm. Split version.

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

Split PS Intein tTA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4 mIL-17 2kb promoter

Plasmid

#20124

Depositor

Warren Strober

Article

Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

Insert

mIL-17 (Il17a Mouse)

Use

Luciferase

Available Since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-mNeptune2-N

Plasmid

#41645

Purpose

Expresses far-red FP mNeptune2 fused to Dronpa145N. Dronpa145N oligomerizes under violet light (390-400nm) and dissociates under cyan light (490-500nm)

Depositor

Michael Lin

Article

Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

Insert

mNeptune2-Dronpa145N

Expression

Mammalian

Mutation

K145N on Dronpa (allows control of photochromic F…

Promoter

CMV

Available Since

Dec. 21, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
Z-lock αTAT

Plasmid

#175290

Purpose

light-controlled analog of the alpha-tubulin acetylase αTAT

Depositor

Klaus Hahn

Article

Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

Insert

Z-lock αTAT

Tags

FLAG

Expression

Mammalian

Mutation

αTAT (236 amino acids)

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-SYP-HRP

Plasmid

#117185

Purpose

Plasmid name in publication: pAAV-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

SYP-HRP

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-tTA

Plasmid

#175280

Purpose

AAV vector mediating neuron-specific expression of tTA

Depositor

Wei Xu

Article

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

tTA advanced (improved tetracycline-controlled transactivator)

Use

AAV

Promoter

Synapsin

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-CRY2-iSH2

Plasmid

#66839

Purpose

Expression of a light-inducible PI3-kinase, generated by fusing the iSH2 domain of human p85α (residues 420–615) to mCherry-CRY2. Binds endogenous PI3-kinase p110α catalytic subunits constitutively.

Depositor

Pietro De Camilli

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

iSH2

Tags

mCherry-CRY2

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRS-LacI-dCas9

Plasmid

#220194

Purpose

mini-Tn7 dCas9 expression vector - LacI/pLac promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9

Use

CRISPR

Expression

Bacterial

Mutation

Pseudomonas aeruginosa codon optimized

Promoter

LacI/pLac

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RabV CVS-N2c(deltaG)-APEX2-Venus

Plasmid

#73467

Purpose

Expresses APEX2 fused to Venus for transsynaptic tracing and the EM analysis of infected neurons.

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

APEX2-Venus

Use

Neurotropic virus

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
ER-HA-mNG21-10

Plasmid

#141165

Purpose

Split Fluorescent Reporter for ER Retrograde Trafficking

Depositor

Wayne Lencer

Article

Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

Insert

ER-HA-mNG21-10-KDEL

Use

Lentiviral

Tags

Signal Sequence, KDEL and HA

Expression

Mammalian

Mutation

K128M, S142T, R150M, G172V and K213M

Promoter

Ef1a

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
NLS-dCas9-HA-NLS--GFP-p300

Plasmid

#183925

Purpose

p300 HAT core domain coupled to catalytically dead dCas9 for localization; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

dCas9-GFP-p300

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST26-USP47

Plasmid

#64326

Purpose

Mammalian expression and purification of USP47.

Depositor

Grigory Dianov

Article

Parsons et al Mol Cell. 2011 Mar 4;41(5):609-15. doi:

Insert

USP47 (USP47 Human)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDN34

Plasmid

#61343

Purpose

mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Tags

GS linker-AsLov2-biNLS2 and PKIt NES

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDN41

Plasmid

#61344

Purpose

mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Tags

GS linker-AsLov2-biNLS9 and PKIt NES

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 41,926 results for: tro