We narrowed to 23,942 results for: Spr

1. pMB67

   Plasmid

   #47947

   Purpose

   Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter

   Depositor

   Mike Boxem

   Article

   Waaijers et al Genetics. 2013 Aug 26.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and egl-13 NLS

   Expression

   Worm

   Mutation

   Promoter

   hsp-16.48

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJF104B

   Plasmid

   #113324

   Purpose

   pTet-dCas9, pTet-MCP-SoxS_R93A

   Depositor

   Jesse Zalatan

   Article

   Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

   Insert

   dCas9 and MCP-SoxS_R93A
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   R93A

   Promoter

   Available since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX458_GABPA_2

   Plasmid

   #64255

   Purpose

   Expresses gRNA against human GABPA along with Cas9 with 2A GFP

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMB63

   Plasmid

   #47945

   Purpose

   Expresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoter

   Depositor

   Mike Boxem

   Article

   Waaijers et al Genetics. 2013 Aug 26.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and egl-13 NLS

   Expression

   Worm

   Mutation

   Promoter

   eef-1A.1 (eft-3)

   Available since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX458_GABPA_1

   Plasmid

   #64254

   Purpose

   Expresses gRNA against human GABPA along with Cas9 with 2A GFP

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJA50

   Plasmid

   #59931

   Purpose

   gRNA for cleavage at unc-58(e665) locus in C elegans

   Depositor

   Andrew Fire

   Article

   Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

   Insert

   unc-58(e665) gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJA54

   Plasmid

   #60212

   Purpose

   cleavage near e1350 locus in sqt-1

   Depositor

   Andrew Fire

   Article

   Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

   Insert

   sqt-1(e1350) gRNA1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pColE1_sgRNA_pos3_AmpR

   Plasmid

   #119146

   Purpose

   Encodes sgRNA targeting position 3 in pColE1_70a_deGFP_KanR

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   sgRNA targeting position 3 in pColE1_70a_deGFP_KanR
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pColE1_sgRNA_pos7_AmpR

   Plasmid

   #119150

   Purpose

   Encodes sgRNA targeting position 7 in pColE1_70a_deGFP_KanR

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   sgRNA targeting position 7 in pColE1_70a_deGFP_KanR
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pColE1_sgRNA_pos5_AmpR

    Plasmid

    #119148

    Purpose

    Encodes sgRNA targeting position 5 in pColE1_70a_deGFP_KanR

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 5 in pColE1_70a_deGFP_KanR
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pColE1_sgRNA_pos2_AmpR

    Plasmid

    #119145

    Purpose

    Encodes sgRNA targeting position 2 in pColE1_70a_deGFP_KanR

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 2 in pColE1_70a_deGFP_KanR
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. PX458_ATF1_1

    Plasmid

    #64690

    Purpose

    Expresses gRNA against human ATF1 along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJA55

    Plasmid

    #59935

    Purpose

    gRNA for cleavage near sqt-1(e1350) locus in C elegans

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    sqt-1(e1350) gRNA2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJET1.2[eb-Tc’bhsp-EGFP-2A-Cre; ye-3xP3-gTc’v-SV40]

    Plasmid

    #124069

    Purpose

    CRISPR/Cas mediated knock-in via non-homologous end-joining in Tribolium; bhsp drives EGFP expression; Dm-ebony and Dm-yellow for linearization; (Transformation plasmid; Black eye marker)

    Depositor

    Gregor Bucher

    Insert

    eb-Tc’bhsp-EGFP-2A-Cre; ye-3xP3-gTc’v-SV40
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Insect

    Mutation

    Promoter

    bhsp68

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCF823_EFS-Cas9-PuroR_lenti

    Plasmid

    #138317

    Purpose

    Lenti expression of SpyCas9 with Puro selection

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    SpyCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAH233

    Plasmid

    #121144

    Purpose

    Expression of gRNA with BbsI placeholder for target cloning

    Depositor

    Katsunori Tanaka

    Article

    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

    Insert

    gRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    S.pombe rrk1

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAH235

    Plasmid

    #121145

    Purpose

    Cas9 expression controlled by the nmt41 promoter

    Depositor

    Katsunori Tanaka

    Article

    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

    Insert

    humanized Streptococcus pyogenes Cas9
    

    Use

    CRISPR

    Tags

    Flag

    Expression

    Yeast

    Mutation

    Promoter

    nmt41

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEL088

    Plasmid

    #137896

    Purpose

    iSTU-CRISPR/Cas12a DR-DR structure crRNA insertion vector with OsCDPK2 intron

    Depositor

    Yong Zhang

    Article

    Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJA14

    Plasmid

    #59929

    Purpose

    gRNA for cleavage at rde-1(H974) locus in C elegans

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    rde-1(H974) gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330-U6-BRD4-HaloTag-Guide-hspCas9

    Plasmid

    #228576

    Purpose

    Expression of Halo-tagged human BRD4 gRNA; CRISPR-mediated gene insertion

    Depositor

    Marcin Imielinski

    Insert

    BRD4 gRNA (BRD4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge