We narrowed to 29,705 results for: LIS
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Plasmid#202186PurposeExpress Arabidopsis thaliana FLS2 gene under UBQ10 promoterDepositorInsertFlagellin sensing 2 (FLS2 Mustard Weed)
UseTagsHAExpressionPlantMutationPromoterAtUBQ10Available sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub(TA)-YPet-NES
Plasmid#84636PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub(TA)-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
FH-TET3-HxDmut-pEF
Plasmid#127896PurposeExpresses ten-eleven-translocation 3 (TET3) containing HxD mutation at H1077Y and D1079ADepositorInsertTET3-HxDmut (TET3 Human)
UseTagsHA and FlagExpressionMammalianMutationPromoterEF-1aAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-V5-PTPN14-PPxY
Plasmid#61006PurposeExpresses human PTPN14 (PPxY domain mutant) in mammalian cells, N-terminus V5 tagDepositorInsertPTPN14 PPxY mutation (PTPN14 Human)
UseTagsV5 tagExpressionMammalianMutationPPxY mutationPromoterCMVAvailable sinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRibo-Sec-APEX2m
Plasmid#176844PurposeExpression of APEX2 in the periplasm from a codon-optimized gene (multi-copy episomal plasmid)DepositorInsertpRibo-Sec-APEX2m
UseInducibleTagsExpressionBacterialMutationPromoterpRibo, theophilline riboswitch inducible hsp60 pr…Available sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV6- Entry-RPL26DC-DDK
Plasmid#133867PurposeExpression of C-terminally FLAG-tagged human RPL26 mutant lacking the C-terminal UFMylation sitesDepositorInsertRPL26DC-myc-FLAG (RPL26 Human)
UseTagsMyc-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4.24-Cd36-enhancer4
Plasmid#138576PurposeFirefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17876439_17877752DepositorInsertCd36 Promoter (Cd36 Mouse)
UseLuciferaseTagsExpressionMutationPromoterMinimal promoterAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BGLF5
Plasmid#195813PurposeGateway Entry Clone of Epstein-Barr virus BGLF5DepositorInsertBGLF5 (BGLF5 Epstein-Barr virus)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
human a5 full length
Plasmid#217818PurposeFull length human integrin a5 expressionDepositorInsertITGA5 (ITGA5 Human)
UseTagsP2A-EGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BRRF2
Plasmid#195820PurposeGateway Entry Clone of Epstein-Barr virus BRRF2DepositorInsertBRRF2 (BRRF2 Epstein-Barr virus)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BXLF1
Plasmid#195825PurposeGateway Entry Clone of Epstein-Barr virus BXLF1DepositorInsertBXLF1 (BXLF1 Epstein-Barr virus)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BHRF1
Plasmid#195780PurposeGateway Entry Clone of Epstein-Barr virus BHRF1DepositorInsertBHRF1 (BHRF1 Epstein-Barr virus)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BARF1
Plasmid#195783PurposeGateway Entry Clone of Epstein-Barr virus BARF1DepositorInsertBARF1 (BaRF1 Epstein-Barr virus)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR U6:gRNA spred1, mitfa:Cas9
Plasmid#118843PurposeTargets spred1 and expresses zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertspred1 gRNA (spred1 Zebrafish)
UseCRISPR; Tol2TagsExpressionMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
VVT1
Plasmid#65779PurposeThe Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNADepositorInsertSaCas9 gRNA backbone, without spacer sequence
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYpet-Optop38i
Plasmid#89749PurposeExpression of light-regulated p38 inhibitor, Ypet-fused, wild-type photosensor, inhibitor MK3BD3-13FDepositorInsertOptop38i3
UseFluorescent proteinTagsYpetExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-17
Plasmid#172747PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-17
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-dCas9-MQ1(C141S)-EGFP
Plasmid#89635PurposeTargeted CpG methylationDepositorInsertsite-specific DNA-methyltransferase SssI
UseCRISPRTags3*FLAG, 6*His, and T2A-EGFPExpressionMammalianMutationC141S, TGC-->TCCPromoterCMVAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lox-stop-Lox p53 R270H
Plasmid#14853DepositorInsertp53 R270H (Trp53 Mouse)
UseCre/LoxTagsLox-STOP-loxExpressionMammalianMutationContact mutant R270H. (Murine p53 codon 270 corre…PromoterAvailable sinceApril 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGL4.24-Cd36-enhancer1
Plasmid#138573PurposeFirefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17835541_17837355DepositorInsertCd36 enhancer (Cd36 Mouse)
UseLuciferaseTagsExpressionMutationPromoterMinimal promoterAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-STAT2
Plasmid#178449Purposedoxycycline-inducible expression of Human STAT2 in mammalian cellsDepositorInsertSTAT2 (STAT2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-ATXN1
Plasmid#16133DepositorInsertAtaxin1 (ATXN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
FH-TET3-CXXCmut-pEF
Plasmid#127895PurposeExpresses ten-eleven-translocation 3 (TET3) containing CXXC mutation 80THQ82 to AAADepositorInsertTET3-CxxCmut (TET3 Human)
UseTagsHA and FlagExpressionMammalianMutationPromoterEF-1aAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRAPPC10_pLENTI-CAG-IRES-GFP
Plasmid#176998PurposeMammalian lentiviral expression vector encoding TRAPPC10DepositorInsertTRAPPC10 (TRAPPC10 Human)
UseLentiviralTagsExpressionMutationPromoterCAGAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
1375_pAAV-U6-SA-WTmLdlrEx14-gRNA2-N22-CB-SACas9-HA-OLLAS-spA
Plasmid#109311PurposePlasmid for AAV SaCas9 Mammalian Expression with a gRNA against LdlrDepositorInsertLdlr gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
NmeCas9_3XNLS_pMCSG7
Plasmid#120078PurposeBacterial expression of wtNmeCas9 with 3X NLSDepositorInsertNmeCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-LMP2A
Plasmid#195816PurposeGateway Entry Clone of Epstein-Barr virus LMP2ADepositorInsertLMP2A (LMP-2A Epstein-Barr virus)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BNLF2a
Plasmid#195767PurposeGateway Entry Clone of Epstein-Barr virus BNLF2aDepositorInsertBNLF2a (BNLF2a Epstein-Barr virus)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BNLF2b
Plasmid#195770PurposeGateway Entry Clone of Epstein-Barr virus BNLF2bDepositorInsertBNLF2b (BNLF2b Epstein-Barr virus)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOAD
Plasmid#196825PurposeYeast two-hybrid ; Gal4 activation domainDepositorTypeEmpty backboneUseTagsGal4 Activation domainExpressionYeastMutationPromoterADHAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMV-LEU2
Plasmid#65334Purposestandard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker LEU2 which could be released and ligated to series of TUsDepositorInsertLEU2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
LLP790_αGCN4-EZH2-FL-CO
Plasmid#211784PurposeSunTag counterpart binding domain, aGCN4, fused to polycomb repressive complex subunit EZH2, with GFP selectionDepositorInsertSnoopCatcher-KRAB
UseTags2xOLLASExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect KRAB e…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-Mac-GFP
Plasmid#58852PurposeAAV-mediated expression of Mac-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) mannerDepositorInsertMac-GFP
UseAAV and Cre/LoxTagsGFPExpressionMammalianMutationPromoterSynAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-RV
Plasmid#188544PurposeGateway entry clone (attL1 & attR5) for LbCas12a-RV mediated genome editingDepositorInsertLbCas12a-RV
UseCRISPR; Gateway compatible lbcas12a-rv entry cloneTagsNLSExpressionPlantMutationG146R, R182VPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-SP110
Plasmid#178448Purposedoxycycline-inducible expression of Human SP110 in mammalian cellsDepositorInsertSP110 (SP110 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-6
Plasmid#172771PurposeBacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-6
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
S100B_pcDNA6.2/EmGFP-Bsd
Plasmid#176952PurposeMammalian expression vector encoding S100B and EmGFP-BsdDepositorInsertS100B (S100B Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
SUMO3_pcDNA6.2/EmGFP-Bsd
Plasmid#176949PurposeMammalian expression vector encoding SUMO3 and EmGFP-BsdDepositorInsertSUMO3 (SUMO3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-hSt3Cas9 (pXK-1721)
Plasmid#208828PurposeCRISPR/hSt3Cas9 expression vector for Animal gene editing, harboring the humanized St3Cas9 gene and the corresponding optimized SP1.gRNA. Key Construct: mU6- SP1.gRNA-CMV-hSt3Cas9.DepositorTypeEmpty backboneUseCRISPR; Stcas9TagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET23A-Fucosidase-E1_10125-Ruminococcus_gnavus-His
Plasmid#207665PurposeExpresses fucosidase E1_10125 from Ruminococcus gnavus in BL21 cells, purified using His-tagDepositorInsertFucosidase E1_10125
UseTagsHis-tagExpressionBacterialMutationamino acids 34-584 were usedPromoterT7 promoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-SUB1
Plasmid#178440Purposedoxycycline-inducible expression of Human SUB1 in mammalian cellsDepositorInsertSUB1 (SUB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL4.24-Cd36-enhancer7
Plasmid#138579PurposeFirefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17918617_17920400DepositorInsertCd36 Promoter (Cd36 Mouse)
UseLuciferaseTagsExpressionMutationPromoterMinimal promoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.24-Cd36-enhancer6
Plasmid#138578PurposeFirefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17898458_17899478DepositorInsertCd36 Promoter (Cd36 Mouse)
UseLuciferaseTagsExpressionMutationPromoterMinimal promoterAvailable sinceMay 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.24-Cd36-enhancer3
Plasmid#138575PurposeFirefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17848003_17849903DepositorInsertCd36 Promoter (Cd36 Mouse)
UseLuciferaseTagsExpressionMutationPromoterMinimal promoterAvailable sinceMay 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.24-Cd36-enhancer2
Plasmid#138574PurposeFirefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17843052_17844983DepositorInsertCd36 Promoter (Cd36 Mouse)
UseLuciferaseTagsExpressionMutationPromoterMinimal promoterAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.24-Cd36-enhancer8
Plasmid#138580PurposeFirefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17923535_17924934DepositorInsertCd36 Promoter (Cd36 Mouse)
UseLuciferaseTagsExpressionMutationPromoterMinimal promoterAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQsupR-Mi2b
Plasmid#15379DepositorInsertshort hairpin RNA against mouse Mi-2 beta (Chd4 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterAvailable sinceApril 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-BLST-Cas9-VPR
Plasmid#115147PurposeSafe harbor site 231 knock-in vector with BlastR-Cas9-VPR expression cassetteDepositorInsertBlastR-P2A-Cas9-VPR
UseCRISPRTagsSV40NLSExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2K-CAGGS-U6-sgRNA-M5-U6-sgRNA-M7-U6-sgRNA-M9-IRES-CFP
Plasmid#114729Purpose3 sgRNAs targeting a sequence upstream of the initiator ATG of the cellular Myc geneDepositorInsertsgRNA M5, sgRNA M7, sgRNA M9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-27
Plasmid#172752PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-27
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only