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We narrowed to 34,443 results for: CaS

Showing: 5851 - 5900 of 34443 results

  1. pEPOR1CB0021

    Plasmid
    #117557
    Purpose
    Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    Promoter_AtU6-26 + sgRNA_NbPDS1 _1mismatch N. benthamiana
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    AtU6-26 (pICSL90002)
    Available since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    117557-plasmid-map-sequence-id-228156

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  2. pEPOR1CB0020

    Plasmid
    #117556
    Purpose
    Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    Promoter_AtU6-26 + sgRNA_NbPDS1 _1mismatch N. benthamiana
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    AtU6-26 (pICSL90002)
    Available since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    117556-plasmid-map-sequence-id-228155

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  3. pEPOR1CB0019

    Plasmid
    #117555
    Purpose
    Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    Promoter_AtU6-26 + sgRNA_NbPDS1 _1mismatch N. benthamiana
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    AtU6-26 (pICSL90002)
    Available since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    117555-plasmid-map-sequence-id-228154

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  4. pEPOR1CB0018

    Plasmid
    #117554
    Purpose
    Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana with 1 mismatch at spacer
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    Promoter_AtU6-26 + sgRNA_NbPDS1 _1mismatch N. benthamiana
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    AtU6-26 (pICSL90002)
    Available since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    117554-plasmid-map-sequence-id-228153

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  5. pEPOR1CB0014

    Plasmid
    #117550
    Purpose
    Level 1 Golden Gate Cassette: AsCas12a expression cassette for dicotyledonous plants
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    2x35S+5'UTR OMEGA (pICH51288) + AsCas12a (with stop codon)+ Nos (pICH41421)
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    2x35S+5'UTR OMEGA (pICH51288)
    Available since
    Nov. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    117550-plasmid-map-sequence-id-228088

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  8. pEPOR2KN0050

    Plasmid
    #117617
    Purpose
    Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    [35S:Sp-eCas9 1.0(pEPOR1CB0010) ] +[AtU6-26:sgRNA]
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    117617-plasmid-map-sequence-id-227712

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  9. pEPOR2KN0051

    Plasmid
    #117618
    Purpose
    Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    [35S:Sp-eCas9 1.1(pEPOR1CB0011) ] +[AtU6-26:sgRNA]
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    117618-plasmid-map-sequence-id-227714

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  15. pJK412

    Plasmid
    #155371
    Purpose
    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    155371-plasmid-map-sequence-id-308999

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  16. 1203F-5’+3'dDR-mCh(22)-GFP-bGHpolyA

    Plasmid
    #221536
    Purpose
    EGFP reporter construct with double unprocessed direct repeat (35nt) at 5' and 3' end, 22nt mCherry-targeting spacer in between DRs
    Depositor
    Omar Akbari
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221536-plasmid-map-sequence-id-437446

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  17. psicheck_200_300_CA_repeat

    Plasmid
    #65880
    Purpose
    Luciferase assay vector, assessing regulation of 200-300 nucleotide CA repeats
    Depositor
    Thomas Tuschl
    Insert
    CA repeats 200-300 nucleotides in length
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. psicheck_300_400_CA_repeat

    Plasmid
    #65879
    Purpose
    Luciferase assay vector, assessing regulation of 300-400 nucleotide CA repeats
    Depositor
    Thomas Tuschl
    Insert
    CA repeats 300-400 nucleotides in length
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. psicheck_50_100_CA_repeat

    Plasmid
    #65882
    Purpose
    Luciferase assay vector, assessing regulation of 50-100 nucleotide CA repeats
    Depositor
    Thomas Tuschl
    Insert
    CA repeats 50-100 nucleotides in length
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  24. pRCC-K

    Plasmid
    #81191
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81191-plasmid-map-sequence-id-154417

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  28. pRCC-N

    Plasmid
    #81192
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81192-plasmid-map-sequence-id-158700

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  34. pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

    Plasmid
    #41863
    Purpose
    This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.
    Depositor
    Feng Zhang
    Article
    Cong et al Science. 2013 Jan 3.
    Insert
    U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    41863-plasmid-map-sequence-id-57052

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  43. pCRISPomyces-1

    Plasmid
    #61736
    Purpose
    Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA
    Depositor
    Huimin Zhao
    Article
    Cobb et al ACS Synth Biol. 2014 Dec 8.
    Inserts
    sSpCas9
    tracrRNA
    crRNA cassette
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    BbsI-flanked lacZ cassette inserted in place of s…
    Promoter
    gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI
    Available since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    61736-plasmid-map-sequence-id-256988

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  50. pJK511

    Plasmid
    #155384
    Purpose
    dCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenus
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Promoter
    Available since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    155384-plasmid-map-sequence-id-309004

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Showing: 5851 - 5900 of 34443 results