165,385 results
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pSL1765 (pSPIN-R, R*MmeI)
Plasmid#160734PurposeSingle-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
ExpressionBacterialPromoterJ23119Available SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Basic Vector
Plasmid#212936PurposeFirefly luciferase vector for investigating regions controlling transcriptionDepositorTypeEmpty backboneUseLuciferaseMutationWTAvailable SinceJan. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYJA5
Plasmid#217778PurposeThe empty vector for quadruple sgRNA cloningDepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsPuromycin resistance gene, T2A, and TagBFPExpressionMammalianPromoterPGK, CMV, U6Available SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBS-EF1afull-H2B-mCherry-P2A-Fluc
Plasmid#207253PurposeLentiviral transfer genome encoding H2B-mCherry-P2A-Fluc driven by a full-length EF1a promoterDepositorInsertH2B-mCherry-P2A-Fluc
UseLentiviralExpressionMammalianPromoterEF1alpha_fullAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2/8
Plasmid#112864PurposeAAV packaging plasmid expressing Rep/Cap genesDepositorInsertRep2/Cap8
UseAAVPromoterTruncated P5Available SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXR003: CasRx gRNA cloning backbone
Plasmid#109053PurposehU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRExpressionMammalianPromoterhU6Available SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-puro
Plasmid#21915Purpose3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralAvailable SinceAug. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDS221
Plasmid#34981PurposeExpresses a high-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.DepositorInsertePDZb1–mCherry
ExpressionYeastPromoterTEFAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro
Plasmid#8453Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiExpressionMammalianAvailable SinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
pTargetF
Plasmid#62226PurposeConstitutive expression of sgRNA without donor editing template DNADepositorInsertsgRNA
UseCRISPRExpressionBacterialPromoterpij23119Available SinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Blast
Plasmid#118055PurposeUsed for expression of sgRNA in mammalian cellsDepositorTypeEmpty backboneExpressionMammalianAvailable SinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJBL7045
Plasmid#167216Purposeoverexpression of CueR in E. coli for cell-free copper sensingDepositorInsertCueR
ExpressionBacterialAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBL7062
Plasmid#167231Purposecell-free cascaded copper sensorDepositorInsertpCue-AKSIRV
UseSynthetic BiologyTags6X HisAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBL7044
Plasmid#167215Purposeoverexpression of PbrR in E. coli for cell-free lead sensingDepositorInsertPbrR
ExpressionBacterialAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBL7061
Plasmid#167230Purposecell-free cascaded lead sensorDepositorInsertpPbr-AKSIRV
UseSynthetic BiologyTags6X HisAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDS220
Plasmid#34980PurposeExpresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.DepositorInsertePDZb–mCherry
ExpressionYeastPromoterTEFAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJBL7056
Plasmid#167225Purposeorthogonal T7 RNAP sfGFP reporterDepositorInsertpAKSIRV-sfGFP
UseSynthetic BiologyAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBL7085
Plasmid#167249Purposeorthogonal mRFP reporter output for cell-free expressionDepositorInsertpAKSIRV-mRFP1
UseSynthetic BiologyAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTYB1-eIF4A
Plasmid#37225DepositorInserteIF4A
TagsinteinExpressionBacterialPromoterT7Available SinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only
