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pPB-EF1A-mSTING

Plasmid

#229739

Purpose

Expresses mouse STING in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

STING (Sting1 Mouse)

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-hARdelta538-614

Plasmid

#89107

Purpose

mammalian expression of human androgen receptor with deletion of amino acids 538-614, deletion of AR DNA binding domain

Depositor

Elizabeth Wilson

Article

Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23

Insert

hAR-538-614 (AR Human)

Use

Tags

Expression

Mammalian

Mutation

hAR delta 538-614

Promoter

CMV

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only
MIG-Luciferase-IRES-GFP

Plasmid

#75021

Purpose

Encodes the expression of Luciferase and GFP

Depositor

Saradhi Mallampati

Insert

Luciferase

Use

Luciferase and Retroviral

Tags

Expression

Mutation

Promoter

Available since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-hARdeltaNH2-6

Plasmid

#89104

Purpose

mammalian expression of human androgen receptor amino acids 507-919

Depositor

Elizabeth Wilson

Article

Simental et al J Biol Chem. 1991 Jan 5;266(1):510-8.

Insert

hAR DNA binding domain+ligand binding domain (AR Human)

Use

Tags

Expression

Mammalian

Mutation

NH2 terminal deletion, contains amino acids 507-…

Promoter

CMV

Available since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-IP- SCAT3.2

Plasmid

#172497

Purpose

SCAT3.2 is fluorescent reporter (FRET probe) for cell survival/apoptosis with Caspase-3 sensitive sequence which can be introduced to mammalian cells by retrovirus vector.

Depositor

David Scadden

Article

Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

Insert

Amcyan

Use

Retroviral

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

MLV LTR

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ASAP3-WPRE

Plasmid

#132331

Purpose

expresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

Depositor

Michael Lin

Article

Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

Insert

ASAP3

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-SMN

Plasmid

#37057

Depositor

Greg Matera

Article

Shpargel et al Proc Natl Acad Sci U S A. 2005 Nov 29;10

Insert

Human SMN1 (SMN1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pSin-EF2-Sox2-Pur

Plasmid

#16577

Depositor

James Thomson

Article

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

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pBTK522-FAST-PETase

Plasmid

#214754

Purpose

plasmid for expression FAST-PETase in Pseudomonas putida

Depositor

Hal Alper

Article

Lu et al Nature. 2022 Apr;604(7907):662-667. doi:

Insert

FAST-PETase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_RUNX1_RUNX1T1

Plasmid

#205915

Purpose

Express mEGFP-tagged fusion protein, RUNX1_RUNX1T1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

RUNX1_RUNX1T1 (RUNX1, RUNX1T1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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GalT-RpHLuorin2

Plasmid

#171719

Purpose

Trans-Golgi expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to B4GALT1 (B4GALT1 Human)

Use

Tags

ratiometric pHluorin2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-OptoSTIM1

Plasmid

#70159

Purpose

Mammalian expression plasmid of OptoSTIM1 (optogenetically-activated STIM1 protein)

Depositor

Won Do Heo

Article

Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

Insert

OptoSTIM1 (STIM1 Mustard Weed, Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223_SETDB1_WT

Plasmid

#82291

Purpose

Gateway Donor vector containing SETDB1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SETDB1 (SETDB1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pWPI-N1ICD

Plasmid

#185525

Purpose

Constitutive overexpression of human NOTCH1 intracellular domain

Depositor

Sean Palecek, Eric Shusta

Article

Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

Insert

NOTCH1 intracellular domain (NOTCH1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF-1a

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR

Plasmid

#126705

Purpose

donor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Article

Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

Insert

GFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-RiboL1-jGCaMP8m

Plasmid

#192617

Purpose

AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

FLEX-RiboL1-jGCaMP8m

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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HA-HIF1alpha-wt-pBabe-puro

Plasmid

#19365

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

wt human cDNA

Promoter

Available since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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pRSETa mEos2

Plasmid

#20341

Purpose

bacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)

Depositor

Loren Looger

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

mEos-2

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pH2rU3_ForInd_Omicron_sinobiological_Spiked21_T7_CMV_ZsGT2APurR

Plasmid

#204146

Purpose

Lentivirus backbone with BA.1 spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Inserts

SARS-CoV-2 BA.1 variant spike
zsGreen-T2A-PuR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

21 amino acid cytoplasmic tail deletion

Promoter

CMV and TRE3G

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1444

Plasmid

#143809

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ARID4B (ARID4B Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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