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Showing: 221 - 240 of 66158 results

  1. VP12

    Plasmid
    #72247
    Purpose
    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    72247-plasmid-map-sequence-id-485302

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  2. pAG/MNase

    Plasmid
    #123461
    Purpose
    Expresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.
    Depositor
    Steven Henikoff
    Publication
    Meers et al Elife. 2019 Jun 24;8. pii: 46314. doi: 1
    Insert
    Protein A-Protein G-Micrococcal Nuclease
    Tags
    6xHIS and HA
    Expression
    Bacterial
    Promoter
    Lac
    Available since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    123461-plasmid-map-sequence-id-417805

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  3. lentiMPH v2

    Plasmid
    #89308
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.
    Depositor
    Feng Zhang
    Publication
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Insert
    MS2-P65-HSF1_2A_Hygro (RELA Human, Synthetic)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1a
    Available since
    March 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89308-plasmid-map-sequence-id-425605

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  9. SBE4-Luc

    Plasmid
    #16495
    Purpose
    Luciferase reporter containing four copies of the Smad binding site
    Depositor
    Bert Vogelstein
    Publication
    Zawel et al Mol Cell. 1998 Mar . 1(4):611-7.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Smad binding element
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Available since
    April 7, 2008
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    16495-plasmid-map-sequence-id-333563

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  10. lentiSAMv2

    Plasmid
    #75112
    Purpose
    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Publication
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75112-plasmid-map-sequence-id-305764

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  11. pcDNA3-HA-ADH

    Plasmid
    #11610
    Depositor
    Steven Johnson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    aldehyde dehydrogenase (ALDH1A1 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    K412I
    Available since
    July 18, 2007
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11610-plasmid-map-sequence-id-232087

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Showing: 221 - 240 of 66158 results