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Plasmid#170167PurposeMammalian Expression of no signal controlDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationn/aPromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only
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pAG1345 mCherry-FIREtag-DEVDG-MoA
Plasmid#205176PurposeExpresses mCherry-FIREtag-DEVDG-MoA in mammalian cellsDepositorInsertmCherry-cagedFIREtag
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE30-hPolB
Plasmid#70761Purposebacterial expression of human DNA Polymerase beta (POLB)DepositorInsertPOLB (POLB Human)
UseTags6x HisExpressionBacterialMutationR333LPromoterT5/lac operonAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCI152-pLenti-SiT-Cas12a
Plasmid#128405PurposeExpress both AsCas12a and CRISPR arrays on single transcriptsDepositorInsertAsCas12a-Triplex
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-T/O-Ds1-mCherry
Plasmid#112855PurposeDox inducible expression in mammalian cells of Ds1 protein fused to mCherry fluorescent proteinDepositorInsertDCHS1 (DCHS1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterdoxycycline inducible promoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
V5-LOV-Turbo-NES
Plasmid#199545Purposeexpresses LOV-Turbo in the mammalian cytosol, lentiviral vectorDepositorInsertLOV-Turbo
UseLentiviralTagsNES and V5ExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMIG-Aalpha WT
Plasmid#10884DepositorInsertPP2A Aalpha (PPP2R1A Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28 His6-mEGFP-D4
Plasmid#226372PurposeProduction of recombinant mEGFP-D4, a fluorescent probe binding cholesterolDepositorInsertD4 (pfoA Clostridium perfringens)
UseTagsHis6 and monomeric EGFPExpressionBacterialMutationPromoterT7Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pET His6 NusA TEV LIC cloning vector (2N-T)
Plasmid#29709DepositorTypeEmpty backboneUseTagsHis6-NusA-TEVExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDB5319
Plasmid#204829PurposeAn integrating plasmid containing both the inducible enotetSW1 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
UseTagsExpressionYeastMutationPromoterCMV promoter and enotetSW1 promoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_hPGK1_EBFP_Donor
Plasmid#178089PurposeHDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.DepositorInsertEBFP (T65S/F145Y)
UseCRISPRTagsExpressionMammalianMutationPromoterHuman PGK1Available sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pArk313
Plasmid#200257PurposeArk313 rep/capDepositorInsertArk313 Rep/Cap
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Mocr TEV LIC cloning vector (1O)
Plasmid#29658DepositorTypeEmpty backboneUseTags6xHis, Mocr, and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
phOct4-EGFP
Plasmid#38776DepositorInsertOct-4 promoter (POU5F1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterhuman OCT4Available sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-PGK-puromycin
Plasmid#51133Purposeexpresses sgRNA from the U6 promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFR-PATagRFP
Plasmid#31950DepositorInsertPATagRFP
UseTagsEGF receptor (EGFR)ExpressionMammalianMutationR69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…PromoterCMVAvailable sinceOct. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET His6 GST TEV LIC cloning vector (2G-T)
Plasmid#29707DepositorTypeEmpty backboneUseTags6xHis, GST, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGWB455
Plasmid#74837PurposeGateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-MD2-HA
Plasmid#133065PurposeRetroviral expression of mouse MD2 tagged with HADepositorInsertMD2 (Ly96 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Target-AIDmax
Plasmid#139107PurposeMammalian Target-AIDmax expression vector (pSI-626)DepositorInsertBi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLCN DSB Repair Reporter (DRR)
Plasmid#98895PurposeDSB Repair Reporter (DRR). Expresses GFP after cut of two inverted ISce1 sites and repair by NHEJ.DepositorInsertNeomycin-T2A-ISce1-ISce1-eGFP
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS316-OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)
Plasmid#198417PurposeExpresses both OsTIR1F74A and mAID-Nb(VHHGFP4) under the control of ADH1 promoter in budding yeastDepositorInsertOsTIR1F74A-T2A-mAID-Nb (VHHGFP4)
UseTagsExpressionYeastMutationPromoterADH1 promoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
FokI-mCherry-LacI-NLS
Plasmid#215096PurposeExpresses FokI-mCherry-LacI-NLS in mammalian cells.DepositorInsertFokI-mCherry-LacI-NLS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Apple-53BP1trunc
Plasmid#69531PurposeFluorescent reporter for DNA damageDepositorInsert53BP1 (TP53BP1 Human)
UseLentiviralTagsApple fluorescent proteinExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CFP
Plasmid#13030PurposeMammalian expression vector for cloning your gene fused to CFPDepositorHas ServiceCloning Grade DNAInsertcyan fluorescent protein
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mBlueberry2-P2A-EGFP
Plasmid#184937PurposeExpresses blue fluorescent protein mBlueberry2 and EGFP in mammalian cellsDepositorInsertmBlueberry2-P2A-EGFP
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTKDP-neo
Plasmid#71320PurposeDonor plasmid - kanamycin resistant donor cassetteDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
KRphi-mRFP
Plasmid#17276DepositorInsertKR phi
UseTagsmRFPExpressionMammalianMutationPromoterAvailable sinceApril 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28-hXRCC1
Plasmid#70759Purposebacterial expression of human XRCC1DepositorInsertXRCC1 (XRCC1 Human)
UseTags6-HISExpressionBacterialMutationPromoterT7/lac operonAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ApoE2
Plasmid#197146PurposeExpresses ApoE2 in Mammalian cellsDepositorInsertApoE2 (APOE Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Rheb (Q64L)-pcw107
Plasmid#64607Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertRHEB (RHEB Human)
UseLentiviralTagsFLAGExpressionMutationQ64LPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
spNW50
Plasmid#173486PurposeExpresses spNW50 in Bl21DepositorInsertspNW50
UseTagsHistagExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
PX458-mcherry
Plasmid#161974PurposeExpresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNADepositorInsertCas9-t2a-mcherry
UseCRISPRTagsExpressionMammalianMutationPromoterCbhAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA
Plasmid#55202PurposePlasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.DepositorInsertdsRed2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pU6a:sgRNA#1
Plasmid#64245Purposeexpresses sgRNA under U6a promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
HDAC7 Flag
Plasmid#13824PurposeMammalian expression of human histone deacetylase 7 with flag tagDepositorInsertHDAC7 (HDAC7 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ucp1-Cre-miR122
Plasmid#228408PurposeExpression of Cre recombinase from a rat Ucp1 enhancer-promoter. Contains miR122 target sequences that suppress expression in hepatocytes.DepositorInsertscre recombinase with SV40 NLS
Cre recombinase with SV40 NLS
UseAAVTagsExpressionMutationPromoterrat Ucp1 enhancer-promoterAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgRNA
Plasmid#71409PurposeThere is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBIDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX313-Renilla luciferase
Plasmid#118016PurposeConstitutive expression of Renilla luciferaseDepositorInsertRenilla luciferase
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only