165,947 results

1. pTn7-SCOUT11.B

   Plasmid

   #201005

   Purpose

   pLv1-pUC18R6KT-miniTn7-Gm-TagBFP, GmR, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Insert

   TagBFP
   

   Expression

   Bacterial

   Promoter

   J23104

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDGB3 alpha2 P35S:dCas9:TV:Tnos (GB2047)

   Plasmid

   #160626

   Purpose

   TU for the constitutive expression of dCas9 fused to TV (TALx6 - VP128) activation domains

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   P35s-dCas9:TV-Tnos
   

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKB01_mCherry

   Plasmid

   #69104

   Purpose

   Expresses mCherry (codon adapted for Bacillus subtilis); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

   Depositor

   Jan-Willem Veening

   Article

   Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. TRUPATH Triple Ga12

   Plasmid

   #196061

   Purpose

   Encodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlpha12-RLuc8 (GNA12 Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXs-Sox2

   Plasmid

   #13367

   Purpose

   Retroviral expression of mouse Sox2

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   SRY-box containing gene 2 (Sox2 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-HiBiT-CD81

   Plasmid

   #162593

   Purpose

   N-terminally HiBiT-tagged human CD81 under CAG promoter

   Depositor

   Masaharu Somiya

   Article

   Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

   Insert

   human CD81 (CD81 Human, Synthetic, human)
   

   Tags

   HiBiT

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. p6599 MSCV-IP N-HAonly FOSL1

   Plasmid

   #34897

   Depositor

   Peter Howley

   Article

   White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

   Insert

   FOSL1 (FOSL1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   MSCV LTR

   Available Since

   Feb. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP scFv [N86/38]

   Plasmid

   #182095

   Purpose

   Mammalian Expression of GFP scFv. Derived from hybridoma N86/38.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting GFP
   

   Tags

   HA, His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Pet28a_Synthetic_Human_H2AZ.1

   Plasmid

   #42629

   Depositor

   Joe Landry

   Insert

   Human H2AZ.1 (H2AZ1 Human, Synthetic, Codon Optimized)
   

   Tags

   Untagged

   Expression

   Bacterial

   Mutation

   Codon Optimized

   Promoter

   T7

   Available Since

   March 21, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pX330S-3

    Plasmid

    #58779

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. FKBP-myc

    Plasmid

    #20211

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    FKBP (FKBP1A Human)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pTn7-SCOUT11.Y

    Plasmid

    #201004

    Purpose

    pLv1-pUC18R6KT-miniTn7-Gm-sYFP2, GmR, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Insert

    sYFP2
    

    Expression

    Bacterial

    Promoter

    J23100

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-WIPI3

    Plasmid

    #42527

    Depositor

    James Hurley

    Article

    Baskaran et al Mol Cell. 2012 Aug 10;47(3):339-48. doi:

    Insert

    WIPI3 (WDR45B Human)
    

    Tags

    GST-TEV

    Expression

    Mammalian

    Mutation

    gene sequence is codon optimized for expression i…

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pDESTmycYBX1

    Plasmid

    #19878

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    Y box binding protein 1 (YBX1 Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. eSpCas9(1.1)

    Plasmid

    #71814

    Purpose

    Expresses high specificity SpCas9. Px330-like plasmid.

    Depositor

    Feng Zhang

    Article

    Slaymaker et al Science. 2015 Dec 1. pii: aad5227.

    Has Service

    Cloning Grade DNA

    Insert

    enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPB_TRE3G_scFV-sfGFP-Prdm9CD-CatMut

    Plasmid

    #235586

    Purpose

    Dox-inducible expression of control catalytic-mutant Prdm9 CD fused with scFV

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Prdm9 (Prdm9 Mouse)
    

    Use

    CRISPR

    Mutation

    G282A

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMXs-Oct3/4

    Plasmid

    #13366

    Purpose

    Retroviral expression of mouse Oct3/4

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    POU domain, class 5, transcription factor 1 (Pou5f1 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMH-SFB-BRCA1

    Plasmid

    #99394

    Purpose

    Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA1

    Depositor

    Junjie Chen, Michael Huen

    Article

    Sy et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

    Insert

    BRCA1 (BRCA1 Human)
    

    Tags

    SFB (S protein-FLAG-Streptavidin binding peptide)

    Expression

    Mammalian

    Available Since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-2xFLAG-2xSTREP (empty vector)

    Plasmid

    #172604

    Purpose

    Empty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Type

    Empty backbone

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7RNAP

    Plasmid

    #170101

    Purpose

    expresses T7 RNAP

    Depositor

    Chase Beisel

    Article

    Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

    Insert

    T7-RNA polymerase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    p70a

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pACU2

    Plasmid

    #31223

    Depositor

    Yuh-Nung Jan

    Article

    Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pROF148

    Plasmid

    #155330

    Purpose

    Binary vector for expression of the PULSE system under the control of AtUbi10 promoter. It contains a plant selection cassette. 

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    LB_Tnos-nptII-Pnos_PAtUbi10-SRDX-NLS-EL222-Tnos_PAtUbi10-E-PIF6-NLS-Tnos_PAtUbi10-PhyB-VP16-NLS-Tnos_RB
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pYPQ141-ZmUbi-RZ-As

    Plasmid

    #86196

    Purpose

    AsCpf1 gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    AsCpf1 gRNA cloning site for ribozyme cleavage
    

    Use

    CRISPR

    Tags

    Hammerhead ribozyme and HDV ribozyme

    Expression

    Plant

    Promoter

    Maize ubiquitin 1

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pcDNA3.1-GINKO1

    Plasmid

    #113112

    Purpose

    Mammalian expression of genetically encoded green fluorescent potassium ion indicator GINKO1

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    GINKO1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pEGFP-hsRPA14 (MSW#503)

    Plasmid

    #208069

    Purpose

    Expression of GFP-tagged hsRPA3 in human cells

    Depositor

    Marc Wold

    Article

    Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

    Insert

    RPA3 (RPA3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. eGFP-TRF1 pWzl-Hygro

    Plasmid

    #19834

    Depositor

    Titia de Lange

    Article

    Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

    Insert

    Telomere-Repeat binding Factor 1 (Terf1 Mouse)
    

    Use

    Retroviral

    Tags

    enhanced GFP

    Available Since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

27. pCK007 pME KalTa4

    Plasmid

    #195965

    Purpose

    Gateway pME Gal4 variant

    Depositor

    Alexa Burger, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    KalTA4 (Gal4)
    

    Use

    Other

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. pGL3Basic-ME.2/ApoEpromoter

    Plasmid

    #51436

    Purpose

    This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.2 enhancer

    Depositor

    Sohail Tavazoie

    Article

    Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:

    Insert

    APOE apolipoprotein E (APOE, ME2 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    The ME.2 enhancer is fused upstream of the ApoE g…

    Available Since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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29. pDONR221_SLC25A39

    Plasmid

    #131970

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A39 (SLC25A39 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 18, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pET30b-7+-SrtA

    Plasmid

    #105602

    Purpose

    expressed 7+ sortase A variant in E.coli

    Depositor

    Stephanie Dougan

    Article

    Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

    Insert

    S.aureus Sortase A, 7+ mutant
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge