161,873 results

1. CROPSeq-PP7-p65-HSF

   Plasmid

   #196719

   Purpose

   CROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).

   Depositor

   Bernhard Schmierer

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-E-Syt1

   Plasmid

   #66830

   Purpose

   Mammalian Expression, Bacterial Expression

   Depositor

   Pietro De Camilli

   Article

   Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

   Insert

   E-Syt1 (ESYT1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3puro-EGFP-FXR1-isoform b

   Plasmid

   #225449

   Purpose

   Express EGFP-fusion protein of FXR1 isoform b

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FXR1 isoform b (FXR1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. LPC-flag-REST-FS

   Plasmid

   #41904

   Depositor

   Stephen Elledge

   Article

   Westbrook et al Nature. 2008 Mar 20;452(7185):370-4. doi

   Insert

   RE1-silencing transcription factor (REST Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   FS mutant

   Promoter

   Available since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. H2B-iRFP

   Plasmid

   #225533

   Purpose

   Encodes H2B-iRFP

   Depositor

   Jared Toettcher

   Article

   McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

   Insert

   H2B
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223-ACVR1

   Plasmid

   #23822

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   ACVR1 (ACVR1 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pLVX-TIGHT-CYCLINF-Tet-OFF

   Plasmid

   #32977

   Depositor

   Michele Pagano

   Insert

   CYCLIN F (CCNF Human)
   

   Use

   Lentiviral

   Tags

   Flag and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   P-TIGHT

   Available since

   Feb. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pmStayGold_mStayGold_I3

   Plasmid

   #239339

   Purpose

   Expresses the I3-01 nanocage subunit N-terminally tagged with two copies of mStayGold in mammalian cells. Assembles into nanocages tagged with 120 FPs.

   Depositor

   Torsten Wittmann

   Article

   Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

   Insert

   I3-01
   

   Use

   Tags

   mStayGold

   Expression

   Mammalian

   Mutation

   K129A

   Promoter

   CMV

   Available since

   June 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas9-sgAAVS1-1

   Plasmid

   #129726

   Purpose

   Expressing SpCas9 and sgAAVS1-1

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   SpCas9 and sgAAVS1-1
   

   Use

   CRISPR

   Tags

   P2A-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC18-mini-Tn7T

    Plasmid

    #64954

    Purpose

    mini-Tn7 base vector with transcriptional terminator

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. Tol2-lyzC-Gal4-Vp16-Crys-CFP

    Plasmid

    #97144

    Purpose

    For Gal4/UAS expression system

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Insert

    Gal4-Vp16
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMJA284= pSin-Pur-MCS-Puro

    Plasmid

    #116877

    Purpose

    Lentiviral transfer vector containing a multible cloning site

    Depositor

    Marcos Alcocer

    Article

    Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV2-mtIF3 shRNA_TagRFP657

    Plasmid

    #182367

    Purpose

    shRNA targeting mtIF3 mRNA

    Depositor

    Chunghun Lim

    Article

    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

    Insert

    mtIF3 (Mtif3 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pICH47742::p35S-ePEmax3::tEURb7

    Plasmid

    #227692

    Purpose

    Golden Gate level 1, position 2, ePEmax3 expression cassette.

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    ePEmax3 being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV-pDisplay-Halo-T2A-EGFP

    Plasmid

    #223498

    Purpose

    Co-expression of HaloTags to outer surface in mammalian cells coupled with a EGFP transfection marker

    Depositor

    James Frank

    Insert

    HaloTag and EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGP-CMV-jGCaMP8f

    Plasmid

    #162373

    Purpose

    Mammalian expression of ultrafast protein calcium sensor

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8f
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Q315L

    Promoter

    CMV

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CMV-Sa-SauriCas9

    Plasmid

    #135967

    Purpose

    Expresses Sa-SauriCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. AICSDP-23:TJP1-mEGFP

    Plasmid

    #87429

    Purpose

    Homology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    TJP1 Homology Arms with mEGFP-linker (TJP1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    March 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. PB-CRISPR

    Plasmid

    #160047

    Purpose

    For cloning sgRNA to knockout a specific gene in piggybac plasmid

    Depositor

    Xiaojun Lian

    Article

    Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

    Type

    Empty backbone

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Sa-SlugCas9

    Plasmid

    #163797

    Purpose

    Expresses Sa-SlugCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEFIRES NQO1 WT

    Plasmid

    #61735

    Purpose

    Expresses WT human NQO1

    Depositor

    Yosef Shaul

    Article

    Asher et al Genes Dev. 2005 Feb 1;19(3):316-21.

    Insert

    NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    EF1a

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pMXs-C3orf17-gfp

    Plasmid

    #70265

    Purpose

    Expresses C3orf17 in mammalian cells with GFP reporter.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    C3orf17 (NEPRO Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

23. TFORF3041

    Plasmid

    #144517

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX15 (SOX15 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pE-SUMO CRYGD

    Plasmid

    #80755

    Purpose

    Expression of human gamma D-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYGD (CRYGD Human)
    

    Use

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. pSEM253 - sgRNA mosTI hygroR

    Plasmid

    #159827

    Purpose

    Single copy or array insertion by MosTI using split hygroR selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    sgRNA mosTI hygroR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LT3GEPIR

    Plasmid

    #111177

    Purpose

    Tet-ON miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pUC57-PB-PGK-tetR-3xFLAG-HA-2A-Puro

    Plasmid

    #129537

    Purpose

    PiggyBac transposon carrying tetR-3xFLAG-HA-2A-Puro under the PGK promoter.

    Depositor

    Hideyuki Okano

    Article

    Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

    Insert

    tetR-3xFlag-HA-2A-Puro
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    hPGK promoter

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. Anti-WAVE1/SCAR [K91/36R]

    Plasmid

    #128629

    Purpose

    Mammalian Expression Plasmid of anti-WAVE1/SCAR (Human). Derived from hybridoma K91/36.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-WAVE1/SCAR (Human) recombinant mouse monoclonal antibody (WASF1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Lenti-UbiC-GFP-ps

    Plasmid

    #183712

    Purpose

    GFP with palmitoylation signal

    Depositor

    Jeffrey Gerst

    Article

    Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

    Insert

    GFP with palmitoylation signal
    

    Use

    Lentiviral

    Tags

    palmitoylation signal

    Expression

    Mutation

    Promoter

    UbiC

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pBPZpGAL4DBDUw

    Plasmid

    #26233

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    Zip-GAL4DBD
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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