4,134 results

1. pCMV-myc-Atg5

   Plasmid

   #24922

   Depositor

   Toren Finkel

   Article

   Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

   Insert

   Atg5 (ATG5 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTDH3-dCas9

   Plasmid

   #46920

   Purpose

   Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoter

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-Camui-CR

   Plasmid

   #40256

   Depositor

   Michael Lin

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   Camuiα-CR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. APEX2 - Actin in pEGFP

   Plasmid

   #66172

   Purpose

   APEX2 fused to the N-terminus of human beta actin

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   APEX2 - human beta Actin (ACTB Human, Synthetic)
   

   Use

   Tags

   Flag epitope tag (DYKDDDDK)

   Expression

   Mammalian

   Mutation

   APEX2 contains 4 mutations relative to wt soybean…

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6

   Plasmid

   #64222

   Purpose

   Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry linked to the Ad4 E4orf6 gene via P2A

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry-P2A-E4orf6

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CMV-T7-hOsa2

   Plasmid

   #17987

   Depositor

   Naoko Tanese

   Article

   Inoue et al J Biol Chem. 2002 Nov 1. 277(44):41674-8

   Insert

   Osa2 (ARID1B Human)
   

   Use

   Tags

   T7 epitope

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-miR21

   Plasmid

   #21114

   Depositor

   Joshua Mendell

   Article

   Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

   Insert

   miR-21 (MIR21 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Flag-HA-BRCC3

   Plasmid

   #22540

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   BRCC3 (BRCC3 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pY002 (pFnCpf1_min)

   Plasmid

   #69975

   Purpose

   Expresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed.

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   FnCpf1 locus without Cas genes nd artificial promoter
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-Syn-Chronos-GFP

    Plasmid

    #59170

    Purpose

    AAV-mediated expression of Chronos-GFP under the Syn promoter

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV Retrograde, AAV1, and AAV5

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Syn

    Available since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMax-E2F1

    Plasmid

    #16007

    Depositor

    Olimpia Meucci

    Article

    Shimizu et al Neurobiol Dis. 2007 Jan . 25(1):17-26.

    Insert

    E2F1 (E2F1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-C2 RanGAP

    Plasmid

    #13378

    Depositor

    Mary Dasso

    Article

    Joseph et al J Cell Biol. 2002 Feb 18. 156(4):595-602

    Insert

    RanGAP (RANGAP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pN1 Lck-GCaMP5G

    Plasmid

    #34924

    Purpose

    Expresses a fusion between the Lck domain and cytosolic GCaMP5G. This construct targets GCaMP5G to the plasma membrane

    Depositor

    Baljit Khakh

    Article

    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

    Insert

    Lck-GCaMP5G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Plasmid contains N-terminal 26 amino acid membran…

    Promoter

    CMV

    Available since

    Feb. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBabe N-Ras 61K

    Plasmid

    #12543

    Depositor

    Channing Der

    Article

    Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

    Insert

    NRas 61K (NRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    61K constitutively activated.

    Promoter

    Available since

    Sept. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato

    Plasmid

    #51082

    Purpose

    Cre dependent expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Has Service

    AAV1

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1a

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. B23(NPM)

    Plasmid

    #34553

    Depositor

    Mary Dasso

    Article

    Yun et al J Cell Biol. 2008 Nov 17;183(4):589-95.

    Insert

    B23/nucleophosmin (NPM1 Human)
    

    Use

    Tags

    DsRed

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. tdTomato-Lifeact-7

    Plasmid

    #54528

    Purpose

    Localization: Actin, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Article

    Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

    Type

    Empty backbone

    Use

    Tags

    tdTomato-Lifeact

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PGKneoF2L2DTA

    Plasmid

    #13445

    Depositor

    Philippe Soriano

    Article

    Hoch et al Development. 2006 Feb . 133(4):663-73.

    Insert

    PGKneoF2L2DTA
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA4TO-H2B-SunTag24x_v1

    Plasmid

    #60911

    Purpose

    Enocdes a histone H2B fused to the SunTag for single molecule imaging

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    H2B
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FU-tet-o-hSox2

    Plasmid

    #19779

    Depositor

    Konrad Hochedlinger

    Article

    Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

    Insert

    Sox2 (SOX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pT2/BH

    Plasmid

    #26556

    Depositor

    Perry Hackett

    Insert

    pT2/BH
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-eNOS-GFP

    Plasmid

    #22444

    Depositor

    William Sessa

    Article

    Sowa et al J Biol Chem. 1999 Aug 6. 274(32):22524-3

    Insert

    WT eNOS-GFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCDNA3 Flag Jnk2a2

    Plasmid

    #13755

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk2a2 (MAPK9 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJFRC177-10XUAS-FRT>-dSTOP-FRT>-myr::GFP

    Plasmid

    #32149

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    FRT> STOP STOP FRT>
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEZY3

    Plasmid

    #18672

    Purpose

    pcDNA3 converted Gateway Destination vector for mammalian expression

    Depositor

    Yu-Zhu Zhang

    Article

    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-Oct3/4-IRES-Neo

    Plasmid

    #13461

    Depositor

    Shinya Yamanaka

    Article

    Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

    Insert

    Oct3/4 (Pou5f1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P31S

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pNW55

    Plasmid

    #22988

    Depositor

    Detlef Weigel

    Article

    Warthmann et al PLoS One. 2008 . 3(3):e1829.

    Insert

    osa-MIR528
    

    Use

    RNAi; Plant gene inactivation

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Tag5Amyc-GSK3b KD

    Plasmid

    #16262

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    GSK3 beta (GSK3B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Kinase dead: K85A

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TtRMPVIR

    Plasmid

    #27995

    Depositor

    Scott Lowe

    Article

    Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.

    Insert

    TtRMPVIR (shRen)
    

    Use

    RNAi and Retroviral; Fluorescent reporters

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCRY2FL(deltaNLS)-mCherryN1

    Plasmid

    #26871

    Purpose

    Mammalian expression of cytoplasmic CRY2-mCherry

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    NLS sequences deleted

    Promoter

    CMV

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge