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8,885 results

Showing: 5121 - 5160 of 8885 results

  1. pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)

    Plasmid
    #103870
    Purpose
    High specificity version of dPspCas13b-ADAR2DD(E488Q) fusions. T375G mutation in the ADAR deaminase domain confers increased specificity with slightly reduced activity.
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GSGGGGS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    103870-plasmid-map-sequence-id-199178

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  3. pSAM_Cam

    Plasmid
    #91570
    Purpose
    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.
    Depositor
    Harry Mobley
    Article
    Bachman et al MBio. 2015 Jun 9;6(3):e00775. doi: 10.11
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only

  8. PX855

    Plasmid
    #62887
    Purpose
    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)
    Depositor
    Feng Zhang
    Article
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Insert
    SpCas9 (aa 2-535)
    Use
    CRISPR
    Tags
    FRB and NES PTK2
    Expression
    Mammalian
    Mutation
    Aspartic acide 10 to Alanine (D10A)
    Promoter
    CBh
    Available Since
    March 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    62887-plasmid-map-sequence-id-105910

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  15. pcDNA3-TLR2-CFP

    Plasmid
    #13015
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 2 (TLR2 Human)
    Tags
    CFP
    Expression
    Mammalian
    Available Since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13015-plasmid-map-sequence-id-471621

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  25. pET_RTX(exo-)-6xHis

    Plasmid
    #145028
    Purpose
    pET vector for IPTG-inducible, T7-based expression of the exonuclease deficient version of RTX bearing a C-terminal 6xHis tag
    Depositor
    Andrew Ellington
    Article
    Bhadra et al BioProtocol 2021
    Insert
    RTX(exo-)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    N210D
    Promoter
    T7
    Available Since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    145028-plasmid-map-sequence-id-292638

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Showing: 5121 - 5160 of 8885 results