162,309 results

1. ER-J-TAG (pGS730)

   Plasmid

   #160038

   Purpose

   pLV-A4-UCOE-EF1-3xHA-sfGFP-JPH-TMD-IRES-BLAS-2A-SNAP-WPRE-A2

   Depositor

   David Sabatini

   Insert

   3xHA-sfGFP-JPH-TMD
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1A eIF4A3

   Plasmid

   #83035

   Purpose

   mammalian expression of eIF4A3

   Depositor

   Gideon Dreyfuss

   Article

   Chan et al RNA. 2004 Feb;10(2):200-9.

   Insert

   eIF4A3 (EIF4A3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

3. EF1a-ABCD3-RFP-Puro

   Plasmid

   #170168

   Purpose

   Mammalian Expression of Ef1a-ABCD3-RFP-Puro

   Depositor

   Christopher Moxham

   Insert

   ABCD3-RFP-Puro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-CAG-PinkyCaMP

   Plasmid

   #232860

   Purpose

   An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under CAG promoter

   Depositor

   Olivia Masseck

   Article

   Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

   Insert

   PinkyCaMP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TFORF2195

   Plasmid

   #142181

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MLXIPL (MLXIPL Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV_BiPVe4_eGFP

   Plasmid

   #221463

   Purpose

   AAV construct expressing eGFP driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_eGFP

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. B-gTEMP/pcDNA3

   Plasmid

   #188447

   Purpose

   B-gTEMP for mammalian expression

   Depositor

   Takeharu Nagai

   Article

   Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

   Insert

   B-gTEMP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAG303-Gal-PA50

   Plasmid

   #84906

   Purpose

   induce expression of dipeptide repeat PA in yeast

   Depositor

   Aaron Gitler

   Article

   Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

   Insert

   PA50 (C9orf72 Synthetic)
   

   Use

   Tags

   FLAG and Myc

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   April 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCSII-EF/mt-(n1)StayGold

   Plasmid

   #185823

   Purpose

   A soluble photostable fluorescent marker of the mitochondrial matrix. The StayGold gene has codon optimization for mammalian expression.

   Depositor

   Atsushi Miyawaki

   Article

   Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

   Insert

   mt-(n1)StayGold
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSH424-ssr

    Plasmid

    #198026

    Purpose

    Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    T0 terminator - Sm resistance cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SmR promoter

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GFP-RhoA

    Plasmid

    #23224

    Depositor

    Channing Der

    Article

    Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

    Insert

    RhoA (RHOA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pHR OVA/p2a/mCherry-CaaX

    Plasmid

    #113030

    Purpose

    for lentivirus production of ovalbumin and mcherry-caax

    Depositor

    Ron Vale

    Article

    Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

    Insert

    OVA/p2a/mCherry-CaaX
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAV-CAG-oScarlet-WPRE-BC6-pA

    Plasmid

    #220770

    Purpose

    Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

    Depositor

    Vijay Samineni

    Article

    Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

    Insert

    oScarlert-WPRE-BC6
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mCh-alpha-tubulin

    Plasmid

    #49149

    Purpose

    expression of alpha-tubulin fused to mCherry. Used for visualization of microtubules.

    Depositor

    Gia Voeltz

    Article

    Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

    Insert

    alpha tubulin (TUBA1B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-pCMV-TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA

    Plasmid

    #171617

    Purpose

    AAV Soma-targeted Cal-Light with KA2

    Depositor

    Hyungbae Kwon

    Article

    Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

    Insert

    TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA
    

    Use

    AAV and Synthetic Biology

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TFORF0930

    Plasmid

    #143242

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF83 (ZNF83 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFP5380 LAMP1-SBP- mCherry-FIREtag

    Plasmid

    #216725

    Purpose

    Expresses LAMP1-mCherry-FIREtag in mamallian cells

    Depositor

    Franck Perez

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    LAMP-1 (LAMP1 Human)
    

    Use

    Tags

    FIREtag, Streptavidin Binding Protein (SBP), and …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. lentiCRISPRv2FE-ABE8e-SpRY

    Plasmid

    #213008

    Purpose

    A lentiviral vector expressing the ABE8e-SpRY base editor and an sgRNA cloning site

    Depositor

    Richard Sherwood

    Article

    Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

    Insert

    ABE8e-SpRY-D10A
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A nickase variant of SpRY

    Promoter

    Ef-1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCW57.1 N-term GFP tTA

    Plasmid

    #107551

    Purpose

    All-in-one dox-repressible expression of cDNA with optional N-term GFP tag.

    Depositor

    David Sabatini

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP (optional)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FUGW-Mito-TurboID

    Plasmid

    #200963

    Purpose

    3rd gen lentiviral plasmid for expressing mitochondria localized TurboID with EGFP

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    Mito-TurboID
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCI-ASC-HA

    Plasmid

    #41553

    Depositor

    Kate Fitzgerald

    Article

    Hornung et al Nature. 2009 Mar 26. 458(7237):514-8.

    Insert

    ASC (PYCARD Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate-early enhancer/promoter

    Available since

    Dec. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti_C_Myc_DDK_IRES_Puro_PRKRA

    Plasmid

    #106113

    Purpose

    Lentiviral expression of PRKRA

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    PRKRA (PRKRA Human)
    

    Use

    Lentiviral

    Tags

    DDK (FLAG) and Myc

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV_BiPVe4_dTomato_nlsdTomato

    Plasmid

    #213936

    Purpose

    AAV construct expressing dTomato driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_dTomato_nlsdTomato

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Has Service

    AAV PHP.eB

    Insert

    dTomato_P2A_nls.dTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLenti-Tet

    Plasmid

    #138086

    Purpose

    Empty vector with Tet-response promoter for inducible expression of proteins. Can be transduced using Lentiviral particles

    Depositor

    Eva Frickel

    Article

    Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV promoter with heptamerized tet‐operat…

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCWX-UBI-hTert-PGK-BSD

    Plasmid

    #114316

    Purpose

    Constitutive expression of the hTert protein

    Depositor

    Patrick Salmon

    Article

    Salmon et al Methods Mol Biol. 2013;945:417-48. doi:

    Insert

    hTERT (TERT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJFRC-13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

    Plasmid

    #217515

    Purpose

    Encodes a LexAoP controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

    Depositor

    S. Lawrence Zipursky

    Article

    Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

    Insert

    13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHAGE-VLDLR

    Plasmid

    #116802

    Purpose

    Lentiviral expression of VLDLR

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    VLDLR (VLDLR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. HRV-B3 IRES

    Plasmid

    #189969

    Purpose

    Part 2 plasmid to introduce the HRV-B3 IRES into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    HRV-B3 IRES
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3/Flag-METTL14

    Plasmid

    #53740

    Purpose

    For expression of METTL14 (methyltransferase-like 14) in human cells

    Depositor

    Chuan He

    Article

    Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

    Insert

    Methyltransferase-like 14 (METTL14 Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pT2/shp53/GFP4

    Plasmid

    #20208

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    shp53/GFP4
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TFORF1034

    Plasmid

    #143745

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    WT1 (WT1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEF5B-FRT-AP-Sstr3-GFP-DEST

    Plasmid

    #49098

    Purpose

    Expressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines

    Depositor

    Maxence Nachury

    Article

    Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

    Insert

    Sstr3 (Sstr3 Mouse)
    

    Use

    Tags

    GFP and acceptor peptide (AP)

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Nov. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET-6xHis-(-30)GFP

    Plasmid

    #62936

    Purpose

    Expression of 6xHis-(-30)GFP in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (-30)GFP
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-hSyn-GRAB-HaloDA1.0

    Plasmid

    #234408

    Purpose

    Expresses the chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0 in neurons

    Depositor

    Yulong Li

    Article

    Zheng et al Science. 2025 Jun 5;388: 6751. doi: http

    Insert

    GPCR activation based chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV 3xFLAG-GABARAPL2 WT

    Plasmid

    #123103

    Purpose

    Expresses 3xFLAG-GABARAPL2 wild-type in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLacO-ISce1

    Plasmid

    #58505

    Purpose

    provides 256 Lac operator repeats adjacent to the ISce1 restriction site

    Depositor

    Tom Misteli

    Article

    Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

    Insert

    Lac repeats adjacent to 6 copies of the ISce1 restriction site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    no promoter

    Available since

    Oct. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. mVenus C1

    Plasmid

    #27794

    Depositor

    Steven Vogel

    Article

    Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

    Has Service

    Cloning Grade DNA

    Insert

    mVenus C1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. paGFP-Omp25

    Plasmid

    #69598

    Purpose

    expresses GFP-labeled mitochondrial outer membrane protein 25

    Depositor

    David Sabatini

    Article

    Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

    Insert

    paGFP-Omp25 (Synj2bp Rat)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    photoactivatable GFP fused to rat Omp25 C-terminus

    Promoter

    CMV

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. HDAC7 Flag

    Plasmid

    #13824

    Purpose

    Mammalian expression of human histone deacetylase 7 with flag tag

    Depositor

    Eric Verdin

    Article

    Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

    Insert

    HDAC7 (HDAC7 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pME tdTomato-CAAX

    Plasmid

    #135204

    Purpose

    Red fluorescent tag

    Depositor

    David Tobin

    Article

    Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

    Insert

    tdTomato
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. wt HA-dynamin 1 pcDNA3.1

    Plasmid

    #34682

    Depositor

    Sandra Schmid

    Insert

    dynamin 1ba (DNM1L Human)
    

    Use

    Tags

    HA (variant)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. mCherry-Clathrin LC-15

    Plasmid

    #55019

    Purpose

    Localization: Clathrin Vesicles, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Clathrin LC (CLTB Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. ZipGFP-Casp3

    Plasmid

    #81241

    Purpose

    A ZipGFP-based Caspase-3 protease reporter T2A mCherry

    Depositor

    Xiaokun Shu

    Article

    To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

    Insert

    ZipGFP with caspase cleavage sequence T2A mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. FUW-Luc-mCherry-puro

    Plasmid

    #183685

    Purpose

    Lentiviral vector expressing luciferase and mCherry.

    Depositor

    Andrew Kung

    Article

    Kimbrel et al Mol Imaging. 2009 May-Jun;8(3):140-7.

    Insert

    Luc-E2A-mCherry-T2A-PuroR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Human Ubiquitin C (UbC) promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. GRM1-DuET

    Plasmid

    #213300

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GRM1 (GRM1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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46. TFORF3081

    Plasmid

    #144557

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ELK3 (ELK3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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47. pFastBac Dual α1B/β3 Tubulin

    Plasmid

    #170552

    Purpose

    Encodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.

    Depositor

    Tarun Kapoor

    Article

    Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

    Inserts

    TUBA1B (TUBA1B Human)
    TUBB3 (TUBB3 Human)
    

    Use

    Tags

    AP linker, GS linker (GGSGG), His10 tag, L21 enha…

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. FLAG-MATR3

    Plasmid

    #32880

    Depositor

    Yossi Shiloh

    Article

    Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

    Insert

    MATR3 Matrin3 (MATR3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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49. pEvolvR-enCas9-PolI5M

    Plasmid

    #113078

    Purpose

    Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    John Dueber, David Schaffer

    Article

    Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

    Insert

    enCas9-PolI5M
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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50. UbcH5c S22R pET28a

    Plasmid

    #12644

    Depositor

    Rachel Klevit

    Article

    Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

    Insert

    Ubch5c - Ubiquitin conjugating enzyme (UBE2D3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 31, 2007

    Availability

    Academic Institutions and Nonprofits only