We narrowed to 23,108 results for: CRISPR

1. pYPQ141D2.0

   Plasmid

   #99906

   Purpose

   Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   OsU3

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

   Plasmid

   #74010

   Purpose

   Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

   Depositor

   Filippo Del Bene

   Article

   Di Donato et al Genome Res. 2016 Mar 8.

   Inserts

   5xUAS
   Cas9
   Cre
   U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pWRS_1001

   Plasmid

   #192205

   Purpose

   lentiviral expression of SpCas9 and two pol III promoters for dual expression of sgRNAs

   Depositor

   William Sellers

   Article

   Li et al Nat Commun. 2022 May 5;13(1):2469. doi:

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SpCas9

   Plasmid

   #188489

   Purpose

   Expresses FLAG tagged SpCas9

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKS diaCas9_sgRNA

   Plasmid

   #74923

   Purpose

   Expresses Cas9 codon optimized for Phaeodactylum tricornutum and a sgRNA driven by a U6 promoter

   Depositor

   Per Winge

   Article

   Nymark et al Sci Rep. 2016 Apr 25;6:24951. doi: 10.10

   Inserts

   LHCF2 promoter
   diaCas9
   LHCF1 terminator
   U6 promoter
   sgRNA
   U6 3' region
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Cas9 module and sgRNA module

   Available Since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJYS2_crtYf

   Plasmid

   #85544

   Purpose

   Constitutive transcription of crRNA of crtYf, Spectinomycin resistance

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Insert

   crRNA of crtYf
   

   Use

   CRISPR; Shuttle vector corynebacterium glutamicum…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSL0527 (pDonor)

   Plasmid

   #130634

   Purpose

   Encodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchCAST donor DNA (Mini-Tn)
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. U6-spTRE3G-CMV-mTagBFP2

   Plasmid

   #102854

   Purpose

   A plasmid encoding TRE3G sgRNA (for SpCas9) and mTagBFP

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   sp-TRE3G-sgRNA; mTagBFP2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV hUbC-VP64 dCas9 VP64-T2A-GFP

   Plasmid

   #59791

   Purpose

   Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized VP64 dead Cas9 VP64 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   Available Since

   Oct. 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBAD_TypeIC_Cascade_cas3_invivo

    Plasmid

    #196400

    Purpose

    expresses CRISPR Cascade-Cas3 Type IC proteins for in vivo assay

    Depositor

    David Taylor

    Article

    O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

    Inserts

    Cas5c
    Cas7c
    Cas8c
    Cas3c
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2

    Plasmid

    #64512

    Purpose

    Expression of St1 dCas9-3XTagBFP2 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XmTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PX458_ZNF644_2

    Plasmid

    #86306

    Purpose

    Encodes gRNA for 3' target of human ZNF644

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against ZNF644 (ZNF644 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PX458_ZNF644_1

    Plasmid

    #86305

    Purpose

    Encodes gRNA for 3' target of human ZNF644

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against ZNF644 (ZNF644 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SpdCas9 KRAB

    Plasmid

    #188499

    Purpose

    Expresses FLAG tagged SpdCas9 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/H840A

    Promoter

    EF1a

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYC1640

    Plasmid

    #158719

    Purpose

    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    Sth1 sgRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GFP-itis pSel

    Plasmid

    #195344

    Purpose

    Selection plasmid expressing non-fluorescent EGFP A111V

    Depositor

    Alexis Komor

    Article

    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    A111V

    Promoter

    J23119

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAS9i

    Plasmid

    #141249

    Purpose

    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 (Cas9), pSNR52 (gRNA)

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJL33

    Plasmid

    #227430

    Purpose

    Kanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)

    Depositor

    Pierre Santucci

    Article

    Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:

    Insert

    mWasabi
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJL34

    Plasmid

    #227431

    Purpose

    Kanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)

    Depositor

    Pierre Santucci

    Article

    Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:

    Insert

    tdTomato
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti_MCP-VP64_Hygro

    Plasmid

    #138458

    Purpose

    lenti vector encoding the MS2-VP64 with a 2A Hygro resistance marker

    Depositor

    Jian Xu

    Article

    Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

    Insert

    vp64
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge