We narrowed to 24,352 results for: CRISPR
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Plasmid#190697PurposeConstitutive expressing of Cas9 and inducible expression of lambda RedDepositorInsertCas9 and Lamda Red system
ExpressionBacterialAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAS9c
Plasmid#141250PurposeConstitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.DepositorTypeEmpty backboneUseCRISPRExpressionYeastPromoterTEF1 (Cas9), pSNR52 (gRNA)Available SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCasPA
Plasmid#113347PurposeBacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosaDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHelper_ShCAST_sgRNA
Plasmid#127921PurposeExpresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDM026
Plasmid#216808PurposeVector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and pyrG selectable marker.DepositorTypeEmpty backboneUseCRISPR; Fungal expressionExpressionBacterialAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-VP64 dCas9 VP64-T2A-GFP
Plasmid#59791PurposeCo-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFPDepositorInserthumanized VP64 dead Cas9 VP64 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationD10A and H840AAvailable SinceOct. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Plac-dCas9 (Plac)
Plasmid#105235PurposeTn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Plac promoter for expression in P. aeruginosaDepositorInsertS. pasteurianus dCas9
UseCRISPR and Synthetic BiologyTagsHAExpressionBacterialMutationD10A, H599AAvailable SinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK02
Plasmid#105133PurposeClostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriTDepositorInsertsUpstream & Downstream pyrE deletion region
tetR
Cas9
gRNA
UseE. coli - c. difficile shuttle vectorAvailable SinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
PX551
Plasmid#60957PurposepAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.DepositorInsertSpCas9
UseAAV and CRISPRTagsHAExpressionMammalianPromoterpMecp2Available SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC gRNA Shuttle
Plasmid#47024PurposeEncodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.DepositorInsertgRNA Shuttle
UseCRISPR; Cas9ExpressionPlantMutationG to T cloning mutation at position 323PromoterMedicago truncatula U6.6Available SinceSept. 3, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCas9_GFP
Plasmid#44719PurposeCo-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cellsDepositorInsertCas9-2A-GFP
UseCRISPRTags2A-GFPExpressionMammalianPromoterCAGAvailable SinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
HiFi Cas9
Plasmid#188490PurposeExpresses FLAG tagged HiFi Cas9DepositorInsertCas9
UseCRISPR and LentiviralExpressionMammalianMutationR691APromoterEF1aAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV-U6gRNA(BbsI)-PGKpuro2ABFP
Plasmid#50946PurposeAn empty gRNA expression vectorDepositorInsertNone
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceFeb. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro (PX459)
Plasmid#48139PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.DepositorInserthSpCas9-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianPromoterCbhAvailable SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
LRG (Lenti_sgRNA_EFS_GFP)
Plasmid#65656PurposeLentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6 promoter-driven sgRNA expression and EFS promo…Available SinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pwtCas9-bacteria
Plasmid#44250PurposeaTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdownDepositorInsertwild-type Cas9
UseCRISPRExpressionBacterialPromoterpLtetO-1Available SinceApril 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dCas9-T2A-GFP
Plasmid#53191PurposeCo-expresses human optimized S. pyogenes dCas9 and GFPDepositorInserthumanized dead Cas9 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationD10A and H840APromoterhUbCAvailable SinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_WT
Plasmid#83482PurposeBacterial expression of WT L. buccalis C2c2 CRISPR effector.DepositorInsertLbu_C2c2_WT
UseCRISPRTagsHis6-MBPExpressionBacterialPromoterT7Available SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-Cas9
Plasmid#63154PurposeMiddle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLSDepositorInsertCas9
UseCRISPR; Tol2 middle entry vector for gatewayAvailable SinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lwa_Cas13a_WT
Plasmid#91865PurposeBacterial expression for Cas13aDepositorInsertLwaCas13a
UseCRISPRTagsHis6-MBP-TEV siteExpressionBacterialPromoterT7Available SinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only