165,947 results

1. pAF018 p3E-2A-mCerulean-CAAX

   Plasmid

   #195975

   Purpose

   Gateway p3E 2A fluorophore

   Depositor

   Christian Mosimann

   Article

   Kirchgeorg et al Dev Dyn. 2018 Oct;247(10):1146-1159. doi

   Insert

   2A-mCerulean-CAAX (membrane)
   

   Use

   Other

   Available Since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBPnlsLexA::GADflUw

   Plasmid

   #26232

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   nlsLexA::GADfl
   

   Expression

   Insect

   Available Since

   Oct. 7, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-32a(+)-NRG1B1

   Plasmid

   #120287

   Purpose

   Produce human recombinant NRG1 in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   NRG1-beta 1
   

   Tags

   6x His and S-Tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 promoter

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pdCRISPomyces-2BD

   Plasmid

   #234429

   Purpose

   Plasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning template

   Depositor

   Min-Kyu Oh

   Article

   Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:

   Insert

   Modified dCas9(D10A, H840A) with polyaspartate linking
   

   Expression

   Bacterial

   Mutation

   Linked polyaspartate using glycine-serine linker

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. eSpCas9(1.1)_No_FLAG_AAVS1_T2

   Plasmid

   #79888

   Purpose

   Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Untagged eSpCas9(1.1)

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. HDM_RSV_A2_G_31AACTdel

   Plasmid

   #237352

   Purpose

   Expression plasmid for RSV A2 G

   Depositor

   Jesse Bloom

   Article

   Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

   Insert

   RSV A2 G human codon optimized from Genbank Accesion KT992094.1
   

   Expression

   Mammalian

   Mutation

   31 amino acid cytoplasmic tail deletion

   Promoter

   CMV

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-AAVS1-PGK-Zeo

   Plasmid

   #227271

   Purpose

   Empty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEB2-mScarlet-I

   Plasmid

   #104007

   Purpose

   Plasmid encoding mScarlet-I

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mScarlet-I
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to DsRed (MSKGE AVIKEF… N6A, R…

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-Flag

   Plasmid

   #208051

   Purpose

   Empty vector for mammalian expression, insert a Flag tag (with ATG) at XhoI site of pcDNA3.1 (-), xhoI site at 5' of flag tag destroyed, xhoI site at 3' of flag tag retained. XhoI in-frame with Flag

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Human ICAM-1

    Plasmid

    #8632

    Depositor

    Timothy Springer

    Article

    Staunton et al Cell 1988 Mar 25;52(6):925-33.

    Insert

    ICAM-1 (ICAM1 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lamin A-mRFP

    Plasmid

    #124268

    Purpose

    Wild-type Lamin A, C-terminally tagged with mRFP

    Depositor

    Eric Schirmer

    Insert

    LMNA (LMNA Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDH-TMEM106b-mApple

    Plasmid

    #179385

    Purpose

    Lentiviral vector for expression of TMEM106b fused mApple

    Depositor

    Angus Johnston

    Article

    Rennick et al Nat Commun. 2022 Oct 12;13(1):6023. doi:

    Insert

    TMEM106b - mApple
    

    Use

    Lentiviral

    Tags

    mApple

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLGB13

    Plasmid

    #126618

    Purpose

    Suicide vector for allelic replacement in Bacteroides, erythromycin selection and aTC-inducible ss-Bfe1 counterselection

    Depositor

    Laurie Comstock

    Article

    Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

    Type

    Empty backbone

    Use

    Allellic exchange in bacteria

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGP-CMV-GCaMP6s-CAAX

    Plasmid

    #52228

    Purpose

    Plasma membrane targeted GCaMP6s, calcium sensor

    Depositor

    Tobias Meyer

    Article

    Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

    Insert

    GCaMP6s-CAAX
    

    Tags

    6xHis and CAAX

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVS1-Neo-M2rtTA

    Plasmid

    #60843

    Purpose

    AAVS1 donor vector for genomic targeting

    Depositor

    Rudolf Jaenisch

    Article

    DeKelver et al Genome Res. 2010 Aug;20(8):1133-42. doi:

    Inserts

    M2rtTA
    Neo
    

    Use

    Targeting donor

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTECH-chAcK3RS(IPYE)

    Plasmid

    #104069

    Purpose

    Expression plasmid encoding chAcK3RS(IPYE) and pylT

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Plpp chAcK3RS(IPYE); PproK pyl
    

    Expression

    Bacterial

    Mutation

    Contains activating mutations V31I, T56P, H62Y, A…

    Promoter

    Plpp and PproK

    Available Since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA GFP10-ZIP-GFP11

    Plasmid

    #182243

    Purpose

    Tripartite split-GFP mammalian. Express leucine zipper with GFP10 and GFP11 (self-assembing with GFP1-9)

    Depositor

    Stéphanie Cabantous

    Article

    Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

    Insert

    GFP10-ZIP-11
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Golgi-mEGFP

    Plasmid

    #182877

    Purpose

    Localization: Gal-T - Golgi Complex

    Depositor

    Salvatore Chiantia

    Article

    Petazzi et al J Virol. 2020 Nov 25. pii: JVI.01238-20.

    Insert

    Golgi-mEGFP
    

    Expression

    Mammalian

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKS037 - pCAGGS-3XFLAG-Smc3-mKate2-bpA

    Plasmid

    #156447

    Purpose

    For transient expression of mouse SMC3 tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse SMC3 (Smc3 Mouse)
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pM-ABE

    Plasmid

    #196292

    Purpose

    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding ABE in place of viral GP

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV M antigenome encoding ABE in place of viral GP
    

    Use

    CRISPR

    Tags

    3×flag

    Expression

    Plant

    Mutation

    See depositor comments below

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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