We narrowed to 6,273 results for: nls
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Plasmid#176623Purposeexpresses optofYap in zebrafishDepositorAvailable SinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only
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NLS-4LNDD-pcDNA3.1(+)
Plasmid#172313PurposeExpresses ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS) in mammalian cellsDepositorInsertLambda N peptide - human ADAR2 Deaminase Domain
Tags6xHis, Flag, and Nuclear Localization Signal of S…ExpressionMammalianPromoterCMVAvailable SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-3xnls-Tq-Ca-FLITS
Plasmid#145030PurposepiggyBac vector for expressing a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsert3xnls-Tq-Ca-FLITS
Tags3xnls-Tq-Ca-FLITSExpressionMammalianPromoterCMVAvailable SinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
NESRA-DEVD-BNLS
Plasmid#50849PurposeExpresses a tandem red ddFP heterodimer in mammalian cells, construct 1 for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid GANLS.DepositorInsertddRFP A and ddRFP B
TagsNES sequence LQKKLEELELDE placed after ddGFP A an…ExpressionMammalianPromoterCMVAvailable SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2+_NLS-Tir1-HA
Plasmid#117716PurposemRNA synthesis of NLS-TIR1-HA for injectionDepositorInsertTRANSPORT INHIBITOR RESPONSE 1
TagsHA and NLSExpressionMammalianMutationcodon-optimized TIR1 for murine expressionPromoterSP6Available SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEX.(NLS)iGlucoSnFR.H66A.L276V
Plasmid#238944PurposeGreen glucose sensor, high sensitivity, nuclearDepositorInsertiGlucoSnFR.L276V.K312A
UseLentiviralTagsMyc and NLSExpressionMammalianPromoterCMVAvailable SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLEX.(NLS)iGlucoSnFR.L276V.K312A
Plasmid#238945PurposeGreen glucose sensor, moderate sensitivity, nuclearDepositorInsertiGlucoSnFR.L276V.K312A
UseLentiviralTagsMyc and NLSExpressionMammalianPromoterCMVAvailable SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdPCP-GFP
Plasmid#183934PurposePCP tandem dimer binding to PP7 RNA stem loops; labeled with GFPDepositorInserttdPCP-GFP
TagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available SinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
mApple-PCNA-19-NLS-4
Plasmid#54937PurposeLocalization: Nucleus, Excitation: 568, Emission: 592DepositorAvailable SinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-His:CRY2:nls:VPR
Plasmid#135989PurposeExpresses the CRY2 blue light-inducible domain fused to the VPR transcriptional activation domain in mammalian cellsDepositorInsertsCRY2 domain fused to the VPR activation domain
CRY2 domain fused to the VPR activation domain
TagsHisExpressionMammalianPromoterCMVAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-NLS-PTEN
Plasmid#206984PurposeLentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with NLSDepositorAvailable SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
NLS-LucWT-GFP
Plasmid#127709PurposeThe expressed ORF encodes nucleus location signal with GFP and WT Luciferase.DepositorInsertLuciferase
TagsEGFP and FLAG-NLSExpressionMammalianPromoterCMVAvailable SinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL1180-HR-PUbEBFP2-NLS
Plasmid#176682PurposeExpression of nuclear localized EBFP2 under Ae. aegypti Polyubiquitin promoter (AAEL003888), EBFP2 version of pSL1180-HR-PUbECFP (Addgene #47917)DepositorInsertEBFP2
ExpressionInsectPromoterAe. aegypti poly-ubiquitin (AAEL003888)Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
NLS pEGFP-N3 (1299)
Plasmid#62043Purposemammalian expression of NLS-EGFPDepositorInsertSV40 NLS
TagsEGFPExpressionMammalianPromoterCMVAvailable SinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-TagRFP-NLS
Plasmid#184446PurposeNucleus-targeted TagRFP expression in mammalian cellsDepositorInsertTagRFP-3xNLS
ExpressionMammalianMutationWTPromoterRat EF1αAvailable SinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GT_CAG_3xTurboGFP-NLS
Plasmid#229777PurposeBxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP (3 copies)DepositorInsert3x TurboGFP
UseSynthetic BiologyTagsNLSExpressionMammalianPromoterCAGAvailable SinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pN1-NLS-pHlu
Plasmid#129320PurposepH measurement, nucleusDepositorInsertpHluorin (from H. Imamura)
ExpressionMammalianAvailable SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3xP3-EGFP.vas-int.NLS
Plasmid#60948PurposeDrosophila, p3xP3-EGFP.vas-int.NLS.attB was digested SpeI to get rid of the attB site. used for RMCE. CRISPR.DepositorInsertvas-phiC31 integrase.NLS
UseRmcePromotervasaAvailable SinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ICUE3-NLS
Plasmid#138218PurposeNuclear-targeted FRET-based cAMP indicator.DepositorInsertICUE3-NLS
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLBS.EF NLS-pHlu
Plasmid#129324PurposepH measurement, nucleus, lentivirusDepositorInsertpHluorin (from H. Imamura)
UseLentiviralAvailable SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQC NLS YFP IX
Plasmid#37341DepositorInsertNLS YFP
Available SinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pN1-NLS-QUE37C
Plasmid#129323PurposeATP measurement, nucleusDepositorInsertQUEEN-37C
ExpressionMammalianAvailable SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Myc:P4:gs:nls:ABI
Plasmid#135985PurposeExpresses the P4 coiled coil fused to the ABI abscisic acid-inducible domain in mammalian cellsDepositorInsertP4 coiled-coil fused to the ABI domain
TagsMycExpressionMammalianPromoterCMVAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRvUBC-mEGFP-NLS
Plasmid#205010PurposeThe vector contains 1kbp of the upstream and downstream regions of the ubiquitin c gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the ubiquitin c gene from R. varieornatus
UseTardigrade expressionAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40_scFv_GCN4_sfGFP-VPR-GB1_NLS
Plasmid#79373PurposeRecruits VPR to a compatible Cas9 protein for transcriptional activationDepositorInsertGCN4-sfGFP-VPR
UseCRISPRExpressionMammalianAvailable SinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
MCS:GFPnls-T2a-ER-Cre-ER
Plasmid#200106PurposeVector for LFNGDepositorTypeEmpty backboneUseUnspecifiedAvailable SinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-DsRed-cNLS
Plasmid#138528PurposeInducible expression of DsRed-Express2 with nuclear localization signalDepositorInsertDsRed-Express2
Tags3x SV40 cNLSExpressionMammalianPromoterTRE3GsAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
NLS-HA-2xMCP-KRAB
Plasmid#126589PurposeExpresses MCP (MS2 Coat Protein) fusion to KRAB in mammalian cells, lentiviral backboneDepositorInsert2XMCP-KRAB
UseLentiviralTagsNLS-HAExpressionMammalianPromoterhuman ubiquitin C promoterAvailable SinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
NLS-LgBiT-CaM
Plasmid#183401PurposeExpresses nuclear-localized LgBiT-CaMDepositorInsertLgBiT-CaM
TagsSV40 NLSExpressionMammalianPromoterCMVAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRvACT-mEGFP-NLS
Plasmid#205001PurposeThe vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Ramazzottius varieornatus
UseTardigrade expressionAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQUASp-RedStinger (nlsRFP)
Plasmid#46165PurposeExpression of RedStinger in insectDepositorTypeEmpty backboneExpressionInsectAvailable SinceAug. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AktAR2-NLS
Plasmid#175012PurposeNuclear-targeted AktAR2.DepositorInsertAktAR-NLS
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-PCNA-19-SV40NLS-4
Plasmid#56469PurposeLocalization: Nucleus, Excitation: 488, Emission: 507DepositorAvailable SinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVITRO1-BFP-NLS
Plasmid#184458PurposeNucleus-targeted EBFP2 expression in mammalian cellsDepositorInsertEBFP2-3xNLS
ExpressionMammalianMutationWTPromoterRat EF1αAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_N-DYK_MTHFD1-NLS
Plasmid#133023PurposeNLS sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)DepositorAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
AsCas12a-2C-NLS in pCSDest
Plasmid#126637PurposeExpresses AsCas12a-2C-NLS in mammalian cellsDepositorInsertAsCas12a-2C-NLS
UseCRISPRTags3xHuman influenza hemagglutinin (HA)-TagExpressionMammalianPromoterCMV IE94 promoterAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFP-LacI-NLS
Plasmid#103840PurposeFlourescent marker for lacO arraysDepositorInsertCFP-LacI-NLS
ExpressionMammalianMutationLacI: C19T (silent, L7L), T264C (silent, A88A), C…PromoterSV2Available SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-NLS-ZapCV2-ALA
Plasmid#203665PurposeExpresses zinc dead sensor ZapCV2-Ala in the nucleus of mammalian cellsDepositorInsertZapCV2-Ala zinc dead sensor
TagsSV40 NLSExpressionMammalianMutationCys and His in Zap1 is mutated with AlaPromoterCMV promoterAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-NLStdMCP- tagRFP
Plasmid#86244PurposeMiddle entry vector for inserting promotors for tissue specific expression of NLS-tdMCP-tagRFPDepositorTypeEmpty backboneUseUnspecifiedTagsNLS-tdMCP-tagRFPAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
H_NLS 41kDa (BFP)
Plasmid#201391PurposeNucleocytoplasmic transport probeDepositorInsert(NLS)SV40-BFP-2PrA
ExpressionMammalianAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE3-FNLS(RA)
Plasmid#112671PurposeCMV expression vector for BE3-FNLS construct (codon optimized)DepositorInsertBE3-FNLS
Tags3x FLAGExpressionMammalianMutationNLS sequence at the N-terminus and D10APromoterCMVAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-nlsGFP-P2A
Plasmid#80808Purposenuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2ADepositorInsertminimal cytomegalovirus immediate early enhancer/promoter
Available SinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMEH275 NLS dTomato
Plasmid#168210PurposeMammalian expression of nuclear-localized dTomatoDepositorInsertNLS dTomato
UseLentiviralTagsNLSExpressionMammalianPromoterCMVAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
NLS-LucDM-GFP
Plasmid#127796PurposeThe expressed ORF encodes nucleus location signal with GFP and destabilized mutant Luciferase.DepositorInsertdestabilized mutant (DM) Luciferase
TagsEGFP and FLAG-NLSExpressionMammalianMutationR188Q, R261Q in Firefly Luciferase insertPromoterCMVAvailable SinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQC NLS mPlum IX
Plasmid#37356DepositorInsertNLS mPlum
Available SinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTrex-b-NLS-hSpCas9
Plasmid#62543PurposeExpression of Cas9 in T. cruziDepositorInsertNLS-hSpCas9
UseCRISPR; Trypanosoma cruzi expressionTags3xFLAG and NLSPromoterRibo-HX1Available SinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
NLS-4LNDDE488Q-pCDNA3.1(+)
Plasmid#172314PurposeExpresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)DepositorInsertLambda N peptide - human ADAR2 Deaminase Domain E488Q
Tags6xHis, Flag, and Nuclear Localization Signal of S…ExpressionMammalianMutationchanged the Glutamic acid 488 in the Deaminase Do…PromoterCMVAvailable SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAd-PV-NLS-CD-DsRed
Plasmid#16345DepositorInsertPV-DsRed-NLS-CD (Pvalb Rat)
UseAdenoviralTagsMycExpressionMammalianMutationFunctional Ca2+binding site CD was inactivated by…Available SinceFeb. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PCNA-19-NLS-4
Plasmid#55116PurposeLocalization: Nucleus, Excitation: 587, Emission: 610DepositorAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-NPM NLS1D
Plasmid#13284DepositorAvailable SinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only