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178,651 results

Showing: 4201 - 4230 of 178651 results

  2. pcDNA3-TLR2-CFP

    Plasmid
    #13015
    Depositor
    Doug Golenbock
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Toll-Like Receptor 2 (TLR2 Human)
    Tags
    CFP
    Expression
    Mammalian
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    13015-plasmid-map-sequence-id-471621

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  16. pDG458

    Plasmid
    #100900
    Purpose
    SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100900-plasmid-map-sequence-id-457690

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  18. pcDNA3-PRB

    Plasmid
    #89130
    Purpose
    mammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418
    Depositor
    Elizabeth Wilson
    Publication
    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human progesterone receptor-B (PGR Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89130-plasmid-map-sequence-id-176077

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  23. pDD286 (TagRFP^SEC^3xMyc)

    Plasmid
    #70684
    Purpose
    TagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.
    Depositor
    Bob Goldstein
    Insert
    TagRFP-T-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xMyc and C. elegans codon-optimized TagRFP-T
    Expression
    Worm
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70684-plasmid-map-sequence-id-306412

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  24. MSP1101

    Plasmid
    #65773
    Purpose
    Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335E, and T1337R mutations in C…
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65773-plasmid-map-sequence-id-112657

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  27. pICE-RNaseHI-D10R-E48R-NLS-mCherry

    Plasmid
    #60367
    Purpose
    Plasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Depositor
    Sébastien Britton, Patrick Calsou
    Publication
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RNase HI-D10R-E48R
    Tags
    NLS from SV40 T antigen and mCherry
    Expression
    Mammalian
    Mutation
    D10R+E48R; catalyticaly inactive
    Promoter
    CMV-tet
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60367-plasmid-map-sequence-id-456413

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Showing: 4201 - 4230 of 178651 results