165,947 results

1. pINDUCER21 (ORF-EG)

   Plasmid

   #46948

   Purpose

   Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

   Depositor

   Stephen Elledge, Thomas Westbrook

   Article

   Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-HA-Ubiquitin-K6

   Plasmid

   #22900

   Depositor

   Sandra Weller

   Article

   Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

   Insert

   Ubiquitin C (UBC Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K6 only, all other lysines mutated to arginines

   Available Since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. AP1muA-Halo-ALFA-polyA-G418 HR

   Plasmid

   #229681

   Purpose

   Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   genomic sequence AP1M1 locus (AP1M1 Human)
   

   Use

   CRISPR

   Tags

   Halo, ALFA

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSIQ

   Plasmid

   #216238

   Purpose

   All-in One IQ transgene switch, empty vector

   Depositor

   Hyunju Ro

   Article

   Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6_empty-sgRNA(PP7)

   Plasmid

   #232432

   Purpose

   empty gRNA backbone which contains the PP7 aptamer in the scaffold tetraloop

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   PP7-tagged sgRNA scaffold driven by human U6 promoter
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-6xHis-SUMO-MarathonRT

   Plasmid

   #109029

   Purpose

   Group II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”

   Depositor

   Anna Pyle

   Article

   Zhao et al RNA. 2018 Feb;24(2):183-195. doi: 10.126

   Insert

   ERE_01490
   

   Tags

   6xHis-SUMO tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVTHM

   Plasmid

   #12247

   Purpose

   2nd gen lentivector expressing shRNA from H1 promoter and GFP. For direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from pSUPER. Dox-controllable if used with tTRKRAB expressing plasmid

   Depositor

   Didier Trono

   Article

   Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. Factor IX donor

   Plasmid

   #182141

   Purpose

   Factor IX donor for twinPE and Bxb1 mediated insertion at ALB

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   attP-Factor IX intron 1 and exon 2-8 CDS and exon 8 3'UTR
   

   Expression

   Mammalian

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pfwB

   Plasmid

   #37329

   Depositor

   John Schiller

   Article

   Buck et al J Virol. 2005 Mar;79(5):2839-46.

   Insert

   GFP
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-flag-GFP-iCAM-1-Tether

    Plasmid

    #205187

    Purpose

    This vector encodes of a synCAM (iCAM1 Tether) and GFP

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM iCAM1 tether and GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

    Plasmid

    #104394

    Purpose

    Yeast MCP-GFP expression

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    MCP-NLS-2xyeGFP
    

    Expression

    Bacterial and Yeast

    Mutation

    MCP fused to NLS(SV40) and 2x yeast codon optimiz…

    Promoter

    CYC1

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-DeAct-GS1

    Plasmid

    #89445

    Purpose

    Expresses EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells

    Depositor

    Brad Zuchero

    Article

    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

    Insert

    DeAct-GS1 (GSN Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Truncation spanning cytoplasmic Gsn amino acids 5…

    Promoter

    CMV

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21a-MTF-His

    Plasmid

    #124133

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Methionyl-tRNA formyltransferase (fmt Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. plenti6-SLC7A11/xCT-V5

    Plasmid

    #170427

    Purpose

    Eepresses SLC7A11 (also named xCT) in mammalian cells

    Depositor

    Boyi Gan

    Article

    Koppula et al J Biol Chem. 2017 Aug 25;292(34):14240-1

    Insert

    solute carrier family 7 member 11 (SLC7A11 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

    Plasmid

    #138150

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    hU6_Rfxcas13d-DR1_BsmBI
    

    Use

    Lentiviral

    Mutation

    DR1 A>T

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1-MAFB-IRF8-CEBPA

    Plasmid

    #190720

    Purpose

    For microglia differentiation from iPSC

    Depositor

    Li Gan

    Article

    Drager et al Nat Neurosci. 2022 Aug 11. pii: 10.1038/

    Insert

    MAFB-IRF8-CEBPA
    

    Expression

    Mammalian

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330.puro

    Plasmid

    #110403

    Purpose

    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance

    Depositor

    Sandra Martha Gomes Dias

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. MaLionR

    Plasmid

    #113908

    Purpose

    Red ATP sensor

    Depositor

    Tetsuya Kitaguchi

    Article

    Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

    Insert

    MaLionR
    

    Expression

    Mammalian

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC013 - Twinstrep-SUMO-huLwCas13a

    Plasmid

    #90097

    Purpose

    Twinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LwCas13a
    

    Use

    CRISPR

    Tags

    6xHis-Twin Strep-SUMO

    Expression

    Bacterial

    Available Since

    June 13, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. ph7SK-gRNA

    Plasmid

    #53189

    Purpose

    Expresses the S. pyogenes sgRNA from the 7SK promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human 7SK

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLPCX2-MT1-MMP-eGFP

    Plasmid

    #89819

    Purpose

    Express in MT1-MMP mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    MT1-MMP (MMP14 Human)
    

    Use

    Retroviral

    Tags

    eGFP

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. qTAG-AAVS1-Ef1a-Blast

    Plasmid

    #227267

    Purpose

    Empty AAVS1 targeting donor for the insertion of Blast and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3.1/hChR2(H134R)-EYFP

    Plasmid

    #20940

    Purpose

    Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    CMV

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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24. pSRKBB-empty

    Plasmid

    #59449

    Purpose

    Contains BioBrick prefixed and suffixed insert with a modified lac promoter, Shine-Delgarno sequence, 5’ & 3’ MCS, 3’ 6xHis tag.

    Depositor

    Claudia Schmidt-dannert

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    modified lac

    Available Since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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25. EF1a-MVPH

    Plasmid

    #235594

    Purpose

    Expresses the MS2-VP64-P65-HSF1 under the control of human EF1a promoter

    Depositor

    Ting Zhou

    Article

    Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

    Insert

    MS2-VP64-P65-HSF1
    

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1a

    Available Since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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26. pORTMAGE-Ec1

    Plasmid

    #138474

    Purpose

    Enables high-efficiency recombineering in E. coli

    Depositor

    George Church

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

    Inserts

    CspRecT
    mutL E32K
    

    Expression

    Bacterial

    Mutation

    E32K

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. pCMV-PE7-P2A-hMLH1dn

    Plasmid

    #222997

    Purpose

    Mammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7-P2A-hMLH1dn
    

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Deletion of residues 754-756 in MLH1 as described…

    Promoter

    CMV

    Available Since

    Aug. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCRIS-PITChv2-BSD-dTAG (BRD4)

    Plasmid

    #91792

    Purpose

    PITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

    Depositor

    James Bradner, Behnam Nabet

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    BSD-P2A-2xHA-FKBP_F36V
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promoterless

    Available Since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pcDNA-HDAC6-FLAG

    Plasmid

    #30482

    Depositor

    Tso-Pang Yao

    Article

    Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

    Insert

    HDAC6 (HDAC6 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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30. tet-AID-PcrA M6-UGI-T2A-mTagBFP2-PGK-Puro-T2A-rtTA

    Plasmid

    #221234

    Purpose

    Tet-inducible lentiviral HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-PcrA M6-UGI-T2A-mTagBFP2
    

    Use

    Lentiviral

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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