163,606 results

1. Su9-EGFP

   Plasmid

   #23214

   Depositor

   David Chan

   Article

   Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

   Insert

   Subunit 9 of the F0-ATPase
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   contains AA 1-69 of subunit 9

   Promoter

   Available Since

   Jan. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 FlipGFP(Mpro) T2A mCherry

   Plasmid

   #163078

   Purpose

   Expresses FlipGFP-based activity reporter of SARS-CoV-2 main protease Mpro and also mCherry

   Depositor

   Xiaokun Shu

   Article

   Li et al PLoS Pathog. 2021 Sep 3;17(9):e1009898.

   Insert

   FlipGFP-based Mpro activity reporter and mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pN251

   Plasmid

   #188471

   Purpose

   Lentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)

   Depositor

   Le Cong

   Article

   Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

   Insert

   enCas12a-HF (gift from Dr. Keith Joung)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSIP403

   Plasmid

   #122028

   Purpose

   Plasmid for expression of gene of interest in Lactobacillus plantarum and L. sakei

   Depositor

   Lars Axelsson, Geir Mathiesen

   Article

   Sorvig et al Microbiology. 2005 Jul;151(Pt 7):2439-49

   Insert

   gusA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   sppA

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSFDuet1_CoiA1_CoiB

   Plasmid

   #208761

   Purpose

   Precursor peptide CoiA1 and synthase CoiB, for the co-expression of CoiA1 in E.coli.

   Depositor

   Wilfred van der Donk

   Article

   Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

   Inserts

   CoiA1
   CoiB
   

   Use

   Tags

   Hisx6 Tag and SUMO

   Expression

   Bacterial

   Mutation

   Changed Ser 20 to Asn, Changed Arg 84 to Lys, bot…

   Promoter

   T7

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3/td5StayGold(c4)=GianCreg

   Plasmid

   #212022

   Purpose

   For labeling the Golgi apparatus. c4 is the appendage for N-terminal tagging with td5StayGold. ‘GianCreg’ is an abbreviation for giantin C-terminal region. ‘=’ denotes a linker [(GGGGS)3].

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GianCreg (GOLGB1 Human)
   

   Use

   Tags

   td5StayGold(c4)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSAM2_mCherry_Gata6

   Plasmid

   #72694

   Purpose

   Doxycyline regulated expression of Gata6 in mammalian cells

   Depositor

   Timothy Kamp

   Article

   Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

   Insert

   Gata 6 (Gata6 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-Doxycycline

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-CMV-dnMCAK

   Plasmid

   #205993

   Purpose

   Over-expression of GFP-dnMCAK

   Depositor

   Floris Foijer

   Article

   Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

   Insert

   dnMCAK
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLminP_Luc2P_RE1

   Plasmid

   #90335

   Purpose

   NFkB - p50/p65 pathway gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   NFKB

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV9:cTNT::3Flag-hYAP S127A

    Plasmid

    #86558

    Purpose

    To produce cardiac specific adeno-associated virus expressing activated human YAP.

    Depositor

    William Pu

    Article

    Lin et al Circ Res. 2014 Jul 18;115(3):354-63. doi

    Insert

    YAP (YAP1 Human)
    

    Use

    AAV

    Tags

    3Flag

    Expression

    Mutation

    Serine 127 was mutated into Alanine, to activate …

    Promoter

    cTNT

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP- SEC61B

    Plasmid

    #121159

    Purpose

    Mammalian expression of GFP tagged SEC61B, used as a general ER marker

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Insert

    SEC61B (SEC61B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. MLV Gag-RFP

    Plasmid

    #1814

    Depositor

    Walther Mothes

    Article

    Sherer et al Traffic 2003 Nov;4(11):785-801.

    Insert

    Gag (gag MuLV)
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 19, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHCMV_SFV_E1E2

    Plasmid

    #207308

    Purpose

    Mammalian expression of Semliki Forest virus E1E2 protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    SFV_E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLPO2

    Plasmid

    #209976

    Purpose

    Contains Level 0 Part: replication origin (SH71rep) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    replication origin (SH71_rep)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pFUGW mCherry-KASH

    Plasmid

    #131505

    Purpose

    mCherry_KASH epression under hUBC promotor; serves as backbone for ORANGE lenti knock-in

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    mCherry_KASH
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUBC

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. T7_CC_PE7_IVT_Template

    Plasmid

    #223022

    Purpose

    Template for in vitro transcription of PE7. For HSPC-related experiments.

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Template for in vitro transcription

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP‐RNASEH1

    Plasmid

    #108699

    Purpose

    For expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cells

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    RNASEH1 (nuclear isoform) (RNASEH1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. px330_Rosa_sgRNA

    Plasmid

    #97007

    Purpose

    Expresses Cas9 and Rosa26 locus specific sgRNA

    Depositor

    Russell Ray

    Article

    Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

    Insert

    Rosa26 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti.PGK.chFP.W

    Plasmid

    #51008

    Purpose

    Lentivector that expresses chFP from internal human PGK promoter

    Depositor

    Rusty Lansford

    Insert

    mChFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human PGK

    Available Since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-NbALFA

    Plasmid

    #159987

    Purpose

    For the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Insert

    mCherry-NbALFA
    

    Use

    Tags

    8histidine-HRV3C protease cleavage site

    Expression

    Bacterial

    Mutation

    3GS linker between mCherry and NbALFA

    Promoter

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. CAG-FR-GECO1c

    Plasmid

    #163682

    Purpose

    Far-red fluorescent calcium indicator FR-GECO1c

    Depositor

    Robert Campbell

    Article

    Dalangin et al bioRxiv 2020.11.12.380089

    Insert

    FR-GECO1c
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPB-cT3G-cERP2-TCF21

    Plasmid

    #192894

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of TCF21

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    TCF21 (TCF21 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. p3xnls-mScarlet3_N1

    Plasmid

    #189775

    Purpose

    Construct for labelling cell nuclei in mammalian cells using nuclear targeted mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    3xnls-mScarlet3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. RN3P-KDM5B-FLAG

    Plasmid

    #86398

    Purpose

    FLAG-tagged mouse KDM5B cDNA clone for mammalian cell expression

    Depositor

    Wei Xie

    Article

    Zhang et al Nature. 2016 Sep 14;537(7621):553-557. d

    Insert

    KDM5B (Kdm5b Mouse)
    

    Use

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Lac

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHR-FUSN-mCh-Cry2olig

    Plasmid

    #101224

    Purpose

    FUS(1-214) fused to mCh-Cry2olig

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    FUS(aa1-214) (FUS Human)
    

    Use

    Lentiviral

    Tags

    mCherry-Cry2olig

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCMV-PEmax-SpG

    Plasmid

    #211549

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

    Insert

    PEmax-SpG
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMBP-MS2

    Plasmid

    #65104

    Purpose

    can be used to isolate RNA binding complexes

    Depositor

    Josep Vilardell

    Article

    Macias et al Mol Cell. 2008 Jun 20;30(6):732-42. doi:

    Insert

    MS2
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    double mutation (V75Q and A81G) that prevents oli…

    Promoter

    tac

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CLIMP-63 pcDNA

    Plasmid

    #80977

    Purpose

    mammalian expression of CLIMP-63

    Depositor

    Peter Steyger

    Article

    Karasawa et al Cell Death Dis. 2010 Nov 18;1:e102. doi:

    Insert

    CLIMP-63 (CKAP4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. phROSA26-Tet-HA-JSAP1_WT

    Plasmid

    #208772

    Purpose

    Tetracycline inducible expression vector for HA-JSAP1_WT, used as a donor plasmid for HA-JSAP1_WT knock-in at the human ROSA26 locus

    Depositor

    Katsuji Yoshioka

    Article

    Suzuki et al Genes Cells. 2023 Nov 14. doi: 10.1111/g

    Insert

    JSAP1 (MAPK8IP3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Ub-G76V-GFP

    Plasmid

    #11941

    Depositor

    Nico Dantuma

    Article

    Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

    Insert

    Ub-G76V-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ubiquitin fused to N-terminus of GFP. Glycine 76 …

    Promoter

    Available Since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge