163,606 results

1. pCAGGS-flpE-puro

   Plasmid

   #20733

   Purpose

   Mammalian expression of FLPe recombinase from the highly-expressed CAGGS promoter; puro selectable marker

   Depositor

   Rudolf Jaenisch

   Article

   Beard et al Genesis. 2006 Jan . 44(1):23-8.

   Insert

   FLPe recombinase
   

   Use

   Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available Since

   March 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pICSL90002

   Plasmid

   #68261

   Purpose

   Level 0 Golden Gate Part: Promoter U6-26 (Arabidopsis thaliana)

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter: U6-26 (Arabidopsis thaliana)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEFIRES-P-EGFP-ADRP

   Plasmid

   #87157

   Purpose

   Fluorescent protein localized to lipid droplets (LDs)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   ADRP (PLIN2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   mCherry-R TTGGTCACCTTCAGCTTGG

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. M13KO7-ΔM13-ΔgeneIII-ΔgeneVI

   Plasmid

   #80840

   Purpose

   Modified helper phage

   Depositor

   Mark Isalan

   Article

   Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

   Inserts

   M13 gene II
   M13 gene X
   M13 gene V
   M13 gene VII
   M13 gene IX
   M13 gene VIII
   M13 gene I
   M13 gene XI
   M13 gene IV
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p3xFLAG-EYFP

   Plasmid

   #34582

   Depositor

   Celeste Nelson

   Article

   Lee et al EMBO J. 2011 May 24;30(13):2662-74. doi:

   Type

   Empty backbone

   Use

   Tags

   3xFLAG and EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCRISPRa_all-in-one

   Plasmid

   #183695

   Purpose

   Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

   Depositor

   Edward Avezov

   Article

   Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJR89

   Plasmid

   #140096

   Purpose

   sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.

   Depositor

   Jonathan Weissman

   Article

   Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

   Insert

   sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT3-N90-beta-catenin

   Plasmid

   #31785

   Depositor

   Xin Chen

   Article

   Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

   Insert

   beta-catenin (CTNNB1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Deletion of aa 1-90- Constitutitvely Active

   Promoter

   EF1alpha

   Available Since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-Cre-IRES2-GFP

   Plasmid

   #26646

   Purpose

   Mammalian coexpression of nls-Cre and EGFP from the CAG promoter

   Depositor

   Anjen Chenn

   Article

   Woodhead et al J Neurosci. 2006 Nov 29;26(48):12620-30.

   Insert

   Cre
   

   Use

   Cre/Lox

   Tags

   GFP and IRES2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BRCA2 A11.2 gRNA

    Plasmid

    #90550

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BRCA2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    BRCA2 (Guide Designation A11.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mCherry-Golgi-7

    Plasmid

    #55052

    Purpose

    Localization: Golgi Complex, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Golgi (B4GALT1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPB_TRE3G_scFV-sfGFP-Prdm9CD

    Plasmid

    #235577

    Purpose

    Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Prdm9 fused with scFV to programme H3K4me3

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Prdm9 (Prdm9 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAVS1-Neo-M2rtTA

    Plasmid

    #60843

    Purpose

    AAVS1 donor vector for genomic targeting

    Depositor

    Rudolf Jaenisch

    Article

    DeKelver et al Genome Res. 2010 Aug;20(8):1133-42. doi:

    Inserts

    M2rtTA
    Neo
    

    Use

    Targeting donor

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mApple-pBAD

    Plasmid

    #54536

    Purpose

    Localization: Protein Expression Vector , Excitation: 568, Emission: 592

    Depositor

    Roger Tsien, Michael Davidson, Nathan Shaner

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Use

    Tags

    6xHis and mApple

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CGBE1-NG (pBM1246)

    Plasmid

    #140256

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)_NG-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. lenti-SpCas9 puro

    Plasmid

    #104994

    Purpose

    This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLVX-TetOne-zeo_3xHA-TurboID-NLS

    Plasmid

    #200022

    Purpose

    For lentiviral transduction of 3xHA-TurboID-NLS under control of a doxycyline-inducible promoter

    Depositor

    Britt Glaunsinger

    Article

    McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

    Insert

    3xHA-TurboID-NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3Gs

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTBL227 NheI-Cas9-KpnI-5xFlag-FseI-TdT-NotI

    Plasmid

    #126477

    Purpose

    Expresses Cas9-5xFlag-TdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    Cas9
    5xFlag
    TdT (DNTT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed codons to reduce repetitive sequences.

    Promoter

    CMV

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCRISPR-Cas9-ScaligD

    Plasmid

    #125688

    Purpose

    Gene knockout/in for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

    Insert

    streptomyces codon optimized spCas9, sgRNA casette, and ScaligD
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    ermE*/tipA

    Available Since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 6xHis-SNAP-PI(3,4,5)P3 Probe

    Plasmid

    #211514

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteria

    Depositor

    Hannes Maib, David Murray

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    BTK1-PH
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. APLP2-bio-His

    Plasmid

    #51642

    Purpose

    Expresses full-length Amyloid-like protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    APLP2 (APLP2 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAG-oG

    Plasmid

    #74288

    Purpose

    To express oG (optimized Glycoprotein) under CAG promoter

    Depositor

    Edward Callaway

    Article

    Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

    Insert

    oG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    chimeric glycoprotein

    Promoter

    CAG

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA6.2 EmGFP hAQP4(M23)

    Plasmid

    #126464

    Purpose

    Expresses human AQP4 (isoform M23) as an EmGFP fusion protein in mammalian cells

    Depositor

    Markus Reindl

    Article

    Mader et al J Neuroinflammation. 2011 Dec 28;8:184.

    Insert

    Aquaporin 4 (AQP4 Human)
    

    Use

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AAV2-hSyn-G-Flamp1

    Plasmid

    #188569

    Purpose

    green biosensor for cAMP in neurons

    Depositor

    Jun Chu

    Article

    Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

    Insert

    G-Flamp1
    

    Use

    AAV

    Tags

    His tag

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pYPQ-CBE-Cas9n-Act3.0

    Plasmid

    #178955

    Purpose

    It consists of a Cas9 nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Insert

    hA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-GFP-Dub3 mouse

    Plasmid

    #72683

    Purpose

    mammalian expression of GFP-Dub3

    Depositor

    Domenico Maiorano

    Article

    van_der_Laan et al Mol Cell. 2013 Nov 7;52(3):366-79. doi:

    Insert

    Dub3 (Usp17le Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pinducer 20 DN-KASHΔPPPL

    Plasmid

    #129280

    Purpose

    Tet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPL

    Depositor

    Daniel Conway

    Article

    Zhang et al Curr Biol. 2019 Aug 2. pii: S0960-9822(1

    Insert

    SYNE1 (SYNE1 Human)
    

    Use

    Lentiviral

    Tags

    mcherry

    Expression

    Mutation

    the last 4 amino acids (PPPL) of the KASH domain …

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCDNA5/FRT/TO_CEP41-HaloTag7_T2A_EGFP

    Plasmid

    #169327

    Purpose

    Expression of CEP41-HaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    CEP41-HaloTag7 (CEP41 Human)
    

    Use

    Tags

    HaloTag7

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTMB301_ZFcharm_Kv1_Prnp_SPM

    Plasmid

    #220840

    Purpose

    AAV genome expressing ZFcharm Kv1 targeting Prnp; single perfect match self-silencing binding site

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    ZFcharm Kv1
    

    Use

    AAV

    Tags

    HAtag

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDONR223-HCK

    Plasmid

    #23879

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    HCK (HCK Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pmScarlet_Giantin_C1

    Plasmid

    #85048

    Purpose

    In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Giantin (GOLGB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa 3131-3259

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. GW1-Mito-pHRed

    Plasmid

    #31474

    Depositor

    Gary Yellen

    Article

    Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

    Insert

    Cox8-tagged pHRed
    

    Use

    Tags

    4xCox8

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHiUGE smFP-V5 donor (all-in-one) ORF2

    Plasmid

    #200388

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. BCJJ345

    Plasmid

    #117504

    Purpose

    YAO:Cas9:E9 Golden Gate Level 1 Position 2 reverse

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:tagt:YAO:Cas9-3(intron):E9:ttgc:BpiI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtYAO

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV4-ApoE2

    Plasmid

    #87085

    Purpose

    Expresses ApoE2 in Mammalian cells

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    Apolipoprotein E2 (APOE Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFV2-HA

    Plasmid

    #187082

    Purpose

    Expression of ATP synthase, with all Cys changed to Ala, and subunit a-HA tagged, subunit beta-His tagged)

    Depositor

    Steven Vik

    Article

    Bae et al Biochim Biophys Acta. 2009 Sep;1787(9):1

    Insert

    atpBEFHAGDC
    

    Use

    Tags

    6-His beta subunit (atpD) and HA tag subunit a (a…

    Expression

    Bacterial

    Mutation

    All Cys codons changed to Ala.

    Promoter

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA 3.2-FUS-1-526aa-V5

    Plasmid

    #29609

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. DsRed2-Mito-7

    Plasmid

    #55838

    Purpose

    Localization: Mitochondria, Excitation: 558, Emission: 583

    Depositor

    Michael Davidson

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    DsRed2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pMito-mCherry-FRB

    Plasmid

    #59352

    Purpose

    Expresses mCherry-tagged MitoTrap in mammalian cells.

    Depositor

    Stephen Royle

    Article

    Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

    Type

    Empty backbone

    Use

    Tags

    FRB and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pUV15-pHGFP

    Plasmid

    #70045

    Purpose

    encodes a pH-sensitive ratiometric GFP (pH-GFP), which allows for noninvasive measurements of intrabacterial pH in live cells

    Depositor

    Sabine Ehrt

    Article

    Vandal et al Nat Med. 2008 Aug;14(8):849-54. doi: 10.

    Insert

    phGFP
    

    Use

    Mycobacterium tuberculosis expression vector

    Tags

    Expression

    Mutation

    Promoter

    mycobacterial promoter Psmyc

    Available Since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pGWB402

    Plasmid

    #74796

    Purpose

    Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pKrox24(2xD-E_inD)Luc

    Plasmid

    #200109

    Purpose

    Luciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    modified EGR1 promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    highly modified sequence

    Promoter

    2 copies of D-E element in front of EGR1 promoter…

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pGEM4Z-Fluc

    Plasmid

    #183474

    Purpose

    This plasmid can be used to prepare Fluc mRNA by In Vitro Transcription

    Depositor

    Chantal Pichon

    Article

    Perche et al Mol Ther Nucleic Acids. 2019 Sep 6;17:76

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET-NlaCascade-NLS-6xHis

    Plasmid

    #180213

    Purpose

    Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    NlaCas5-Cas8-Cas7
    

    Use

    Tags

    6XHis and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. Tet1-dCas9

    Plasmid

    #136650

    Purpose

    Tet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

    Insert

    Tet1 (Tet1 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-Tet1-dCas9-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pPB_TRE3G_scFV-sfGFP-Kmt5cFL

    Plasmid

    #235581

    Purpose

    Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Kmt5c fused with scFV to programme H4K20me3

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Kmt5c (Kmt5c Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pBAD33-Gm

    Plasmid

    #65098

    Purpose

    bacterial expression vector with Gentamycin selection, based on pBAD33

    Depositor

    Juan Tomas

    Article

    Jimenez et al J Bacteriol. 2009 Apr;191(7):2228-36. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. CROP-sgRNA-MS2

    Plasmid

    #153457

    Purpose

    CROP-seq vector with mCherry and x2 MS2 loops in gRNA scaffold

    Depositor

    Wolf Reik

    Article

    Alda-Catalinas et al Cell Syst. 2020 Jul 22;11(1):25-41.e9. d

    Insert

    sgRNA empty backbone
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. p-EGFP-C1-Flag-mFmr1(wt)

    Plasmid

    #87929

    Purpose

    Expresses mouse FMRP-EGFP fusion, which can be phosphorylated on ser-499.

    Depositor

    Stephanie Ceman

    Article

    Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

    Insert

    Fmr1 (Fmr1 Mouse)
    

    Use

    Tags

    Flag and eGFP

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pEF_PE2

    Plasmid

    #199267

    Purpose

    Mammalian expression of SpCas9 prime editor 2 under EF1α promoter

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    PE2-P2A-mp53DD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Δ40-903

    Promoter

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge