Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

177,170 results

Showing: 7251 - 7300 of 177170 results

  4. pcDNA3-CXCL12-sfGFP

    Plasmid
    #98961
    Purpose
    Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12
    Depositor
    Erik Procko
    Publication
    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CXCL12 (CXCL12 Human)
    Tags
    Superfolder GFP
    Expression
    Mammalian
    Mutation
    Full-length, wildtype
    Promoter
    CMV
    Available since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    98961-plasmid-map-sequence-id-193141

    Enlarge

  5. pQUAST-attB

    Plasmid
    #104880
    Purpose
    For insertion of 5xQUAS-geneX transgenes using PhiC31 integrase system
    Depositor
    Christopher Potter
    Type
    Empty backbone
    Expression
    Insect
    Available since
    March 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    104880-plasmid-map-sequence-id-204757

    Enlarge

  6. pAAV.hSynapsin.SF-iGluSnFR.A184S

    Plasmid
    #106174
    Purpose
    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Publication
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Has service
    AAV1 and AAV9
    Insert
    SF-iGluSnFR.A184S
    Use
    AAV
    Mutation
    GltI: A184S
    Promoter
    hSynapsin
    Available since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    106174-plasmid-map-sequence-id-204113

    Enlarge

  7. pAAV-CBh-mKate2-IRES-MCS (WT-WT)

    Plasmid
    #105921
    Purpose
    AAV-mediated gene expression from CBh promoter. Contains canonical left and right AAV2 ITRs
    Depositor
    Marcella Patrick
    Has service
    AAV2
    Inserts
    mKate2
    IRES-Multiple cloning site
    Use
    AAV
    Mutation
    codon optimized
    Promoter
    CBh and same CBh promoter as mKate2
    Available since
    Feb. 15, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles
    105921-plasmid-map-sequence-id-443051

    Enlarge

  8. phage UbiC NLS HA stdMCP stdHalo

    Plasmid
    #104999
    Purpose
    Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labeling
    Depositor
    Jeffrey Chao
    Publication
    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.
    Insert
    stdMCP-stdHalo
    Use
    Lentiviral
    Tags
    HA tag, NLS, Two synonymous copies of the Halo-ta…
    Expression
    Bacterial and Mammalian
    Promoter
    UbiC
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    104999-plasmid-map-sequence-id-202896

    Enlarge

  9. pEGFP-N-Drd1

    Plasmid
    #104358
    Purpose
    Expresses mouse dopamine receptor subtype 1 with EGFP fused to the C-terminus of the receptor. Localizes to primary cilia.
    Depositor
    Kirk Mykytyn
    Publication
    Domire et al Cell Mol Life Sci. 2011 Sep;68(17):2951-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Dopamine receptor D1 (Drd1) (Drd1 Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    104358-plasmid-map-sequence-id-200988

    Enlarge

  14. pcDNA3.1-hAsCpf1(TYCV) (pY210)

    Plasmid
    #89351
    Purpose
    Expresses humanized AsCpf1 variant that recognizes TYCV PAMs
    Depositor
    Feng Zhang
    Publication
    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cpf1, RR variant
    Use
    CRISPR
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    S542R/K607R
    Promoter
    CMV
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    89351-plasmid-map-sequence-id-178227

    Enlarge

  16. pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2]

    Plasmid
    #84446
    Purpose
    AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.
    Depositor
    Edward Boyden
    Publication
    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi
    Has service
    AAV1, AAV5, and AAV8
    Insert
    Jaws-KGC-tdTomato-ER2
    Use
    AAV
    Tags
    ER2, KGC, and tdTomato
    Expression
    Mammalian
    Mutation
    K200R W214F
    Promoter
    CAG
    Available since
    April 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    84446-plasmid-map-sequence-id-317050

    Enlarge

  20. pPB-US-ECasE

    Plasmid
    #83961
    Purpose
    Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.
    Depositor
    Eleanor Chen
    Insert
    ERT2-spCas9-ERT2
    Expression
    Mammalian
    Mutation
    please see depositor comments below
    Promoter
    U6 (gRNA), EF1a (ERT2-Cas9-ERT2)
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    83961-plasmid-map-sequence-id-214594

    Enlarge

  21. pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)

    Plasmid
    #85008
    Purpose
    For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.
    Depositor
    Takuji Iwasato
    Publication
    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-ires-tTA
    Expression
    Mammalian
    Available since
    Nov. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    85008-plasmid-map-sequence-id-167314

    Enlarge

  22. pFUSEss-CHIg-mG1_M18

    Plasmid
    #82357
    Purpose
    Expression plasmid coding for heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18.
    Depositor
    Joanna Bereta
    Publication
    Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103
    Insert
    mouse immunoglobulin heavy chain IgG1 isotype (Ighg1 Mouse)
    Tags
    none
    Expression
    Mammalian
    Mutation
    none
    Promoter
    hEF1-HTLV prom
    Available since
    Sept. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    82357-plasmid-map-sequence-id-157227

    Enlarge

  26. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

    Plasmid
    #73440
    Purpose
    Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    PG6-vioABE-P4A6-vioC-P3A2-vioD
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    PG6, P4A6, and P3A2 (orthogonal T7-lac variants)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    73440-plasmid-map-sequence-id-135339

    Enlarge

  27. pcDNA3.1+ eiF4A1 myc HIS

    Plasmid
    #71657
    Purpose
    mammalian expression of eiF4A1
    Depositor
    Gideon Dreyfuss
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eiF4A1 (EIF4A1 Human)
    Tags
    HIS and myc
    Expression
    Mammalian
    Mutation
    aa403 from Ala to Arg
    Promoter
    CMV
    Available since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    71657-plasmid-map-sequence-id-422687

    Enlarge

  31. CXCR4-Tango

    Plasmid
    #66262
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CXCR4 (CXCR4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    66262-plasmid-map-sequence-id-148151

    Enlarge

  34. BPK2101

    Plasmid
    #65770
    Purpose
    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    Use
    CRISPR
    Tags
    NLS-3xFlag
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65770-plasmid-map-sequence-id-112650

    Enlarge

  35. pcDNA3-PM-TORCAR

    Plasmid
    #64930
    Purpose
    Plasma membrane-targeted FRET biosensor for monitoring mTORC1 kinase activity.
    Depositor
    Jin Zhang
    Publication
    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn-TORCAR (EIF4EBP1 Synthetic, Human)
    Tags
    Cerulean, N-terminal targeting sequence from Lyn …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    64930-plasmid-map-sequence-id-109694

    Enlarge

  36. Clover-mRuby2-FRET-10

    Plasmid
    #58169
    Purpose
    Localization: Cytosolic FRET, Excitation: , Emission:
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    Clover and mRuby2
    Expression
    Mammalian
    Available since
    April 28, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    4 Articles
    58169-plasmid-map-sequence-id-200604

    Enlarge

  37. pSNAPf-C1

    Plasmid
    #58186
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    SNAP tag
    Expression
    Mammalian
    Available since
    Jan. 6, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    7 Articles
    58186-plasmid-map-sequence-id-314094

    Enlarge

  38. pPHT-PIP2

    Plasmid
    #60937
    Purpose
    Fluorescent FPX sensor for PIP2
    Depositor
    Anne Marie Quinn
    Publication
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PIP2 dimerization dependent sensor RA+B monomers
    Tags
    dimerization dependent fluorescent protein monome…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    60937-plasmid-map-sequence-id-503887

    Enlarge

  40. mCherry-Farnesyl-5

    Plasmid
    #55045
    Purpose
    Localization: Plasma Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Farnesyl (HRAS Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    55045-plasmid-map-sequence-id-314088

    Enlarge

  44. pICherryNeo

    Plasmid
    #52119
    Purpose
    a bicistronic IRES-mCherry containing vector for mammalian expression (CMV promoter)
    Depositor
    Dario Vignali
    Type
    Empty backbone
    Tags
    IRES-mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    52119-plasmid-map-sequence-id-80505

    Enlarge

  45. CaV1.3e[8a,11,31b,Δ32,42a]

    Plasmid
    #49333
    Purpose
    Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].
    Depositor
    Diane Lipscombe
    Publication
    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CACNA1D (Cacna1d Rat)
    Expression
    Mammalian
    Mutation
    Contains exons 8a, 11, 31b and 42a.Deletion of ex…
    Promoter
    CMV
    Available since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    10 Articles
    49333-plasmid-map-sequence-id-159439

    Enlarge

  46. AAV phSyn1(S)-DreO-bGHpA

    Plasmid
    #50363
    Purpose
    Can be used to generate AAV virus that will express DreO recombinase in neurons from the synapsin promoter
    Depositor
    Hongkui Zeng
    Publication
    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10
    Has service
    AAV5 and AAV Retrograde
    Insert
    DreO recombinase
    Use
    AAV
    Tags
    none
    Mutation
    Sequence optimized for expression in mammalian ce…
    Promoter
    hSyn1
    Available since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    50363-plasmid-map-sequence-id-308988

    Enlarge

Showing: 7251 - 7300 of 177170 results