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159,371 results

Showing: 7251 - 7300 of 159371 results

  2. ADA

    Plasmid
    #39020
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ADA (ADA Human)
    Use
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  5. qTAG-C-moxGFP-Puro-LAMP1

    Plasmid
    #207788
    Purpose
    Donor template for moxGFP-2A-Puro insertion into the C-terminus of the LAMP1 locus. For lysosome visualization.To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LAMP1 Homology Arms flanking a moxGFP-Puro Cassette (LAMP1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 1, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207788-plasmid-map-sequence-id-416383

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  6. TFORF3083

    Plasmid
    #144559
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOSL2 (FOSL2 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144559-plasmid-map-sequence-id-404246

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  10. pmal_c5x_RNAse_Inhib

    Plasmid
    #153314
    Purpose
    Bacterial expression of an RNase inhibitor
    Depositor
    Philippa Marrack, Drew Endy
    Insert
    RNAse Inhibitor
    Use
    Tags
    Maltose Binding Protein
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 2, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153314-plasmid-map-sequence-id-298771

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  11. TFORF2200

    Plasmid
    #143352
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    POU4F1 (POU4F1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143352-plasmid-map-sequence-id-400182

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  14. pFC334

    Plasmid
    #87846
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    argB
    yA specific sgRNA
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    A. nidulans gpdA promoter with Hammerhead ribozym…
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87846-plasmid-map-sequence-id-174613

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  16. pTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC

    Plasmid
    #221357
    Purpose
    Mammalian expression of Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC; to be used with Anti-DYKDDDDK [IPI-4E11/M1] light chain (Plasmid 221358) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221357-plasmid-map-sequence-id-436816

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  17. pTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa

    Plasmid
    #221358
    Purpose
    Mammalian expression of Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa; to be used with Anti-DYKDDDDK [IPI-4E11/M1] heavy chain (Plasmid 221357) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221358-plasmid-map-sequence-id-436819

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  19. pUltra-Plus

    Plasmid
    #161775
    Purpose
    3rd generation lentiviral multicistronic vector for mammalian expression of three genes of interest
    Depositor
    Jorge Luis Arias-Arias
    Type
    Empty backbone
    Use
    Lentiviral; Multicistronic
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    UbC
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161775-plasmid-map-sequence-id-316822

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  21. pSC101-Donor

    Plasmid
    #140630
    Purpose
    Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    LE, RE, KanR, temperature sensitive pSC101 origin of replication
    Use
    CRISPR; Transposon
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    140630-plasmid-map-sequence-id-306011

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  25. pLenLox_U6 p53

    Plasmid
    #59360
    Purpose
    Lentiviral expression vector: p53 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.
    Depositor
    Peter Scheiffele
    Article
    Chih et al Science. 2005 Feb 25;307(5713):1324-8. E
    Inserts
    shRNA p53 (mouse) (Trp53 Mouse)
    eGFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and mouse U6
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    59360-plasmid-map-sequence-id-93311

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  31. pLSB-NAT

    Plasmid
    #166698
    Purpose
    Combined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    adh15 promoter and tRNA promoter
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166698-plasmid-map-sequence-id-329407

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  35. pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid
    #50454
    Purpose
    Double floxed Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV8
    Insert
    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50454-plasmid-map-sequence-id-214300

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  42. pCW57-MCS1-P2A-MCS2 (Blast)

    Plasmid
    #80921
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    80921-plasmid-map-sequence-id-234619

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  46. Cas12aUltra_5XNLS

    Plasmid
    #218775
    Purpose
    His-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLS
    Depositor
    Ross Wilson
    Article
    Sahu et al bioRxiv 2024.07.14.603391
    Insert
    5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a
    Use
    CRISPR
    Tags
    6XHis (C terminal), HA, Nucleoplasmin, SV40, and …
    Expression
    Bacterial
    Mutation
    Nucleotides mutations were made to encode for arg…
    Promoter
    Available since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    218775-plasmid-map-sequence-id-443448

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  48. pLL3.7-EF-EYFP-YAP1_5SA-PolyA

    Plasmid
    #112285
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) 5SA active mutant - serines 61, 109, 127, 164 and 397 (also known as 381 in other isoforms)
    Depositor
    Erik Sahai
    Article
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Human YAP1-2gamma with 5 Serines (61, 109, 127, 1…
    Promoter
    EF-1 alpha
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    112285-plasmid-map-sequence-id-227437

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Showing: 7251 - 7300 of 159371 results