161,674 results

1. mCherry-INPP5K

   Plasmid

   #171950

   Purpose

   Expresses INPP5K in mammalian cells

   Depositor

   Cheng-han Yu

   Article

   Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   INPP5K (INPP5K Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLR5-CBh-dCas9-hUtx-IRES-Hyg

   Plasmid

   #122374

   Purpose

   Expresses dCas9-hUtx-IRES-Hyg in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   CBh-dCas9-hUtx-IRES-Hyg-pA
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDisplay-GRAB_OT1.0

   Plasmid

   #185384

   Purpose

   Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   GPCR activation based oxytocin sensor GRAB_OT1.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHR_SFFv_Her2-BFP_RHL027

   Plasmid

   #164823

   Purpose

   Lentiviral vector for constitutive expression of HER2+TM(23-675)-BFP

   Depositor

   Wendell Lim

   Article

   Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

   Insert

   Extracellular and transmembrane region of ERBB2 (ERBB2 Human)
   

   Use

   Lentiviral

   Tags

   tagBFP

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-pTH-iCre-WPREpA

   Plasmid

   #213130

   Purpose

   Truncated rat TH promoter expressing iCre

   Depositor

   Ulrik Gether

   Article

   Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

   Insert

   iCre
   

   Use

   AAV and Mouse Targeting

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   Truncated TH promoter from rat (Rattus norvegicus…

   Available since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLminP_Luc2P_RE28

   Plasmid

   #90370

   Purpose

   TFEB - Lysosomal biogenesis gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TFEB

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNLF1-N_CCT5:1-541

   Plasmid

   #210978

   Purpose

   Mammalian expression of full-length human CCT5. N-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   CCT5:M1-E541 (CCT5 Human)
   

   Use

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pETDuet_huCAD_ICADL (TevS)

   Plasmid

   #100098

   Purpose

   dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences

   Depositor

   Jim Wells

   Article

   Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

   Insert

   DNA fragmentation factor subunit alpha (CAD, DFFA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   two caspase cleavage sites (DETD-117 and DAVD-224…

   Promoter

   T7

   Available since

   Jan. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSLHDR01_mCherry_tCD19

   Plasmid

   #123661

   Purpose

   Construction of 1st donor for scarless genome editing

   Depositor

   Matthew Porteus

   Article

   Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

   Type

   Empty backbone

   Use

   Mouse Targeting; Human targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pWZL Neo Myr Flag PDK1

    Plasmid

    #20564

    Depositor

    William Hahn, Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    PDK1 (PDK1 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJAT10

    Plasmid

    #204293

    Purpose

    For marking CRISPR HDR integrant with DsRed expressed in flight muscles.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    MHC-DsRed
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    MHC

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. miniCMV-(mNeonGreen)4-tDeg

    Plasmid

    #129402

    Purpose

    (mNeonGreen)4-tDeg fluorogenic protein

    Depositor

    Samie Jaffrey

    Article

    Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

    Insert

    (mNeonGreen)-tDeg
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOpen-T4 PNK

    Plasmid

    #165520

    Purpose

    The most commonly used polynucleotide kinase.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    T4 PNK
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. FUW-hSNCA-GFP

    Plasmid

    #215489

    Purpose

    Lentiviral expression and easy visualization of alpha-synuclein in mammalian cells

    Depositor

    Ophir Shalem

    Article

    Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMRX-IZ-ANXA5-mRuby3

    Plasmid

    #184891

    Purpose

    Stable expression of ANXA5-mRuby3 (Annexin A5-mRuby3) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

    Insert

    ANXA5 (ANXA5 Human)
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-C1-PRKAA1

    Plasmid

    #30305

    Depositor

    Jay Brenman

    Article

    Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

    Insert

    AMP-activated Protein Kinase α1 (Prkaa1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ZJOM90

    Plasmid

    #133647

    Purpose

    Integration of SS-mCherry-HDEL in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast endoplasmic reticulum (ER).

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    pTPI1-SS-mCherry-HDEL
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SpCas9-TadCBEd-ivt-template

    Plasmid

    #193843

    Purpose

    Template for in vitro transcription of TadCBEd (with SpCas9)

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadCBEd with SpCas9
    

    Use

    In vitro transcription template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. qTAG-C-moxGFP-Zeo

    Plasmid

    #207732

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. AKAP1-hLOV-TEVcs-GAL4bd-VP64-V5

    Plasmid

    #170995

    Purpose

    HiLITR transcription factor with AKAP1 N-terminal tmd targeting sequence (mitochondria)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    AKAP1(tmd)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (AKAP1 Synthetic)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1-alpha

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRDA_052

    Plasmid

    #136474

    Purpose

    Lentiviral expression of AsCas12a gRNA

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    Puromycin resistance
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 5_T7-GAP43-mScarlet3

    Plasmid

    #225932

    Purpose

    In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the GAP43 membrane localisation signal

    Depositor

    Michalis Averof

    Article

    Karapidaki et al bioRxiv 2024.11.12.623055

    Insert

    GAP43-mScarlet3
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CMV-tdTomato-P2A-paCaMKII

    Plasmid

    #165431

    Purpose

    Expresses tdTomato and paCaMKII in mammalian cells to induce synaptic plasticity.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    tdTomato-P2A-paCaMKII
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGF1-4eCOL2A1

    Plasmid

    #97210

    Purpose

    Lentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoter

    Depositor

    Ryan Porter

    Article

    De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d

    Insert

    4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    COL2A1 regulatory elements

    Available since

    Oct. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. dSpCas9-VPR

    Plasmid

    #205247

    Purpose

    In vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.

    Depositor

    Rasmus Bak

    Article

    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

    Insert

    dSpCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV_S5E2_Chrimson_tdTomato

    Plasmid

    #219432

    Purpose

    AAV construct expressing Chrimson-tdTomato under the control of E2 regulatory element

    Depositor

    Gordon Fishell

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Insert

    Chrimson-tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRSET-mEosEM

    Plasmid

    #132708

    Purpose

    application in Correlative light-electron microscopy

    Depositor

    Pingyong Xu

    Article

    Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    mEosEM
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDONR223_PIK3CA_p.H1047R

    Plasmid

    #82824

    Purpose

    Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Matthew Meyerson, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    H1047R

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLVX TRE 3G BFP

    Plasmid

    #128070

    Purpose

    Lentiviral expression of Tet inducible TagBFP

    Depositor

    Oskar Laur

    Insert

    TagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    no

    Promoter

    Available since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. NFAT luciferase reporter

    Plasmid

    #10959

    Depositor

    Toren Finkel

    Article

    Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

    Insert

    IL-2 promoter (IL2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PX458-3xHA-SpCas9-HF1

    Plasmid

    #201952

    Purpose

    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTTCC

    Plasmid

    #164264

    Purpose

    Expresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilus

    Depositor

    Gudmundur Hreggvidsson

    Article

    Adalsteinsson et al Sci Rep. 2021 May 5;11(1):9586. doi: 10.

    Inserts

    Cas9
    gRNA scaffold
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pcel and trc

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. TFORF2018

    Plasmid

    #142311

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PITX3 (PITX3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEVS104

    Plasmid

    #207393

    Purpose

    Conjugal helper plasmid

    Depositor

    Edward Ruby, Eric Stabb, Jason Peters

    Article

    Hall et al MicroPubl Biol. 2023 Jul 13;2023:10.1791

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLBH531_MBP-Cas14a1 expression

    Plasmid

    #112500

    Purpose

    Expresses MBP fused to Cas14a1

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14a1
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. HSC1-HS4-D4Z4c-GiP

    Plasmid

    #58541

    Purpose

    Derived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoter

    Depositor

    James Ellis

    Article

    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

    Inserts

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    D4Z4 repeat, partial
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1-alpha

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAC-BETAipi

    Plasmid

    #53277

    Purpose

    Contains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

    Insert

    idi
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoter

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pH6-MBP-TEV-BirA

    Plasmid

    #179694

    Purpose

    Expresses His6 tagged MBP-BirA fusion protein

    Depositor

    Mark Arbing

    Insert

    BirA
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMV-hERalpha

    Plasmid

    #101141

    Purpose

    Mammalian expression of full-length human estrogen receptor-alpha

    Depositor

    Elizabeth Wilson

    Article

    Mao et al J Biol Chem. 2008 May 9;283(19):12819-30

    Insert

    estrogen receptor-alpha (ESR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET His6 Sumo TEV LIC cloning vector (2S-T)

    Plasmid

    #29711

    Depositor

    Scott Gradia

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    6xHis, SUMO, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. TFORF2886

    Plasmid

    #143655

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GLI3 (GLI3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pAAV-CAG-eLACCO1.1

    Plasmid

    #168789

    Purpose

    Fluorescent sensor for extracellular lactate

    Depositor

    Marie-Eve Paquet

    Article

    Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

    Insert

    eLACCO1.1
    

    Use

    AAV

    Tags

    ER export and GPI

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. EMTB-mCherry

    Plasmid

    #26742

    Depositor

    William Bement

    Article

    Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

    Insert

    ensconsin (MAP7 Human)
    

    Use

    Probe for microtubules

    Tags

    2x mCherry and Myc

    Expression

    Mammalian

    Mutation

    Microtubule binding-domain of ensconsin (amino ac…

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. NeonGreen-C1-ORP2

    Plasmid

    #220069

    Purpose

    overexpresses ORP2 with a neonGreen fluorophore

    Depositor

    Gerry Hammond

    Article

    Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

    Insert

    OSBPL2 (OSBPL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pmtb-t7-alpha-bungarotoxin

    Plasmid

    #69542

    Purpose

    For in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.

    Depositor

    Sean Megason

    Article

    Swinburne et al PLoS One. 2015 Aug 5;10(8):e0134005. doi

    Insert

    alpha-bungarotoxin
    

    Use

    Tol2, beta-actin promoter, sp6,t7 for zebrafish

    Tags

    Expression

    Mutation

    Promoter

    beta-actin, sp6, t7

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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46. pET28a-AviTag-SnoopLigase

    Plasmid

    #105626

    Purpose

    Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylation

    Depositor

    Mark Howarth

    Article

    Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

    Insert

    AviTag-SnoopLigase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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47. pGRG25

    Plasmid

    #16665

    Depositor

    Nancy Craig

    Article

    McKenzie et al BMC Microbiol. 2006 . 6():39.

    Insert

    mTn7::MCS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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48. pcDNA5/FRT/TO_Cox8a(4x)-SNAP-tag2-T2A-EGFP

    Plasmid

    #226515

    Purpose

    Mamalian cell expression of SNAP-tag2 in the mitochondrial matrix and cytosolic EGFP

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    Cox8a(4x)-SNAP-tag2-T2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. pcDNA3.1_PAsfCherry2(1-10)

    Plasmid

    #82612

    Purpose

    Express PAsfCherry2(1-10) in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    PAsfCherry2(1-10)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Glu 118 to Gln, changed Thr 128 to Ile

    Promoter

    CMV

    Available since

    Jan. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. qTAG-N-Zeo-dTAG

    Plasmid

    #207705

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-dTAG cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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