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We narrowed to 7,632 results for: alp

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Showing: 7251 - 7300 of 7632 results

  3. TFORF3247

    Plasmid
    #144723
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    LEF1 (LEF1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144723-plasmid-map-sequence-id-404303

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  4. plenti-As-Cas12a-2xNLS

    Plasmid
    #155047
    Purpose
    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged _Acidaminococcus sp. BV3L6 (As)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    AsCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS, Myc-tag and SV40 NLS
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Aug. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    155047-plasmid-map-sequence-id-308054

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  6. TFORF1323

    Plasmid
    #141809
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    KLF10 (KLF10 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141809-plasmid-map-sequence-id-413873

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  11. hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

    Plasmid
    #99278
    Purpose
    Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Article
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mVenus-IRES-mCherry
    Puro
    Use
    Lentiviral
    Tags
    H2B, IRES, PGK, mCherry, and mVenus
    Promoter
    EF1 alpha-human and PGK-mouse
    Available Since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99278-plasmid-map-sequence-id-262659

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  13. INCTbiosyn-pEF-GFP(rc)Bxb1

    Plasmid
    #127511
    Purpose
    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase Bxb1 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase Bxb1 attachment sites.
    Expression
    Mammalian
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127511-plasmid-map-sequence-id-250763

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  14. INCTbiosyn-pEF-GFP(rc)phiC31

    Plasmid
    #127510
    Purpose
    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase phiC31 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase phiC31 attachment sites.
    Expression
    Mammalian
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127510-plasmid-map-sequence-id-250757

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  16. pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS

    Plasmid
    #115694
    Purpose
    All-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.
    Depositor
    Erik Sontheimer
    Article
    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:
    Inserts
    NmeCas9
    Nme-sgRNA
    Use
    CRISPR
    Tags
    NLS and NLS, HA
    Expression
    Insect and Mammalian
    Mutation
    None and fusion of repeat/tracrRNA to make a sgRNA
    Promoter
    EF-1 alpha and U6
    Available Since
    Sept. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    115694-plasmid-map-sequence-id-221986

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  18. TFORF1103

    Plasmid
    #142730
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GTF2IRD1 (GTF2IRD1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142730-plasmid-map-sequence-id-395807

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  19. plenti-Lb-Cas12a-2xNLS

    Plasmid
    #155046
    Purpose
    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS, Myc-tag and SV40 NLS
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Aug. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    155046-plasmid-map-sequence-id-308670

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  21. hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

    Plasmid
    #99277
    Purpose
    Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Article
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mOrange2-IRES-EGFP
    Puro
    Use
    Lentiviral
    Tags
    EGFP, H2B, IRES, PGK, and mOrange2
    Promoter
    EF1 alpha-human and PGK-mouse
    Available Since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99277-plasmid-map-sequence-id-197594

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  22. TFORF1322

    Plasmid
    #141808
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    KLF10 (KLF10 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141808-plasmid-map-sequence-id-396950

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  25. Ad:2CMV_A2R2-N25K3

    Plasmid
    #122242
    Purpose
    Expresses a dominant negative Kash construct that disrupts LINC complexes in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone
    Depositor
    Corey Neu
    Article
    Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516
    Inserts
    C-terminal end of Nesprin-3 (Syne3 Mouse)
    Actinin alpha 2 (Actn2 Mouse)
    Use
    Adenoviral
    Tags
    Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    122242-plasmid-map-sequence-id-238909

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  26. pMH014_AAVS1_puro-pA-HS4-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid
    #179436
    Purpose
    Dual fluorescent reporter construct with double HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus
    Depositor
    Lacra Bintu
    Article
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Insert
    HS4-TRE-cit-HS4-mch (DH)
    Expression
    Mammalian
    Promoter
    pEF alpha, pRSV
    Available Since
    Feb. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    179436-plasmid-map-sequence-id-355459

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  27. PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-ins

    Plasmid
    #78099
    Purpose
    Nuclear fluorescent reporter in mammalian cells, phiC31 attB for integration, can be silenced with TetR-CR or rTetR-CR (e.g. CR can be KRAB or DNMT3B).
    Depositor
    Michael Elowitz
    Article
    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do
    Insert
    Histone H2B - Citrine (H2BC21 Synthetic, Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    5xTetO pEF alpha
    Available Since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only

  29. pSLPB2B-ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187950
    Purpose
    ecDHFR degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187950-plasmid-map-sequence-id-373501

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  31. pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187948
    Purpose
    ER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    ER50-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Mutation
    6 mutations, T371A, L384M, M421G, G521R, Y537S, N…
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187948-plasmid-map-sequence-id-373701

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  36. PLL5.0-Anillin ShRNA-Cherry-Anillin deltaActin binding domain

    Plasmid
    #187276
    Purpose
    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Actin binding domain
    Depositor
    Alpha Yap
    Article
    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.
    Insert
    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    Use
    Lentiviral
    Tags
    mCherry
    Mutation
    deltaActin binding domain
    Promoter
    U6, CMV
    Available Since
    Sept. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    187276-plasmid-map-sequence-id-372976

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  38. pMH015_AAVS1_puro-pA-core-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

    Plasmid
    #179437
    Purpose
    Dual fluorescent reporter construct with double core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus
    Depositor
    Lacra Bintu
    Article
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Insert
    coreHS4-TRE-cit-coreHS4-mch (DC)
    Expression
    Mammalian
    Promoter
    pEF alpha, pRSV
    Available Since
    Feb. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    179437-plasmid-map-sequence-id-355553

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  39. PLL5.0-Anillin ShRNA-Cherry-Anillin deltaMyosin binding domain

    Plasmid
    #187275
    Purpose
    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Myosin binding domain
    Depositor
    Alpha Yap
    Article
    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.
    Insert
    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    Use
    Lentiviral
    Tags
    mCherry
    Mutation
    deltaMyosin binding domain
    Promoter
    U6, CMV
    Available Since
    Sept. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    187275-plasmid-map-sequence-id-372978

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  40. TFORF1102

    Plasmid
    #144166
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GTF2IRD1 (GTF2IRD1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144166-plasmid-map-sequence-id-353688

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  43. FSF-GFP

    Plasmid
    #163658
    Purpose
    Expresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from TFR, TMD from ST6GAL1, and luminal domain from TFR in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and transferrin receptor (ST6GAL1, TFRC Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the cytosolic tail from TFR…
    Available Since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163658-plasmid-map-sequence-id-322789

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  44. TFORF3499

    Plasmid
    #144975
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RARA (RARA Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144975-plasmid-map-sequence-id-404542

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  45. pRRLHygro-pEF1a-p53ashL344P-mKate2-splitmVenusC

    Plasmid
    #69587
    Purpose
    Expresses mutated p53-L344P tagged with mKate2 and split C-term mVenus
    Depositor
    Galit Lahav
    Article
    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:
    Insert
    p53-L344P (TP53 Human)
    Use
    Lentiviral
    Tags
    mKate2 and split mVenus - C term
    Expression
    Mammalian
    Mutation
    p53 L344P mutation that prevents dimerization and…
    Promoter
    EF1alpha
    Available Since
    April 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    69587-plasmid-map-sequence-id-135230

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  46. pSLPB2B-SMASh-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187949
    Purpose
    SMASh degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    SMASh-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187949-plasmid-map-sequence-id-373500

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  48. Cav2.2 HA E1658A (EIVA) pcDNA3

    Plasmid
    #206114
    Purpose
    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658A mutation in the P loop of Domain IV which reduces trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Expression
    Mammalian
    Mutation
    E1658A
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206114-plasmid-map-sequence-id-415366

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Showing: 7251 - 7300 of 7632 results