Skip to main content
Addgene
Showing: 5921 - 5960 of 10227 results

  18. pTEV5_ RpMatB_3

    Plasmid
    #78207
    Purpose
    Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - very weak binding
    Depositor
    Martin Webb
    Article
    Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do
    Insert
    matB (His6 C106A T167A R286C Q457C K488A)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    C106A T167A R286C Q457C K488A
    Promoter
    T7
    Available since
    July 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    78207-plasmid-map-sequence-id-152439

    Enlarge

  19. pDC146

    Plasmid
    #169366
    Purpose
    Expresses Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96
    Depositor
    Axel Brunger
    Article
    Cipriano et al J Biol Chem. 2013 Aug 9;288(32):23436-45
    Insert
    Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96
    Use
    Tags
    6 his tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169366-plasmid-map-sequence-id-333023

    Enlarge

  27. NmMetQ N238A

    Plasmid
    #129648
    Purpose
    Expresses N238A mutation in the mature metQ gene from Neisseria meningitides, without the signal sequence
    Depositor
    Douglas Rees
    Article
    Nguyen et al Protein Sci. 2019 Jul 26. doi: 10.1002/p
    Insert
    metQ Gene
    Use
    Tags
    10x Histidine Tag, Enterokinase, and pelB signal …
    Expression
    Bacterial
    Mutation
    Changed Asparagine 238 to Alanine
    Promoter
    T7 Promoter
    Available since
    Sept. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    129648-plasmid-map-sequence-id-254340

    Enlarge

Showing: 5921 - 5960 of 10227 results