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We narrowed to 9,660 results for: Gnas

Showing: 7401 - 7450 of 9660 results

  4. pYC2085

    Plasmid
    #158721
    Purpose
    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364
    Insert
    Sth1 sgRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    158721-plasmid-map-sequence-id-320843

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  14. pCoofy57

    Plasmid
    #122009
    Purpose
    Mammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His, PreScission Cleavage site and different C-tag options
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Use
    Tags
    His8 and Multi1: depending on the LP2 primer, eit…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    122009-plasmid-map-sequence-id-243824

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  24. pTEV5_ RpMatB_1

    Plasmid
    #78205
    Purpose
    Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor
    Depositor
    Martin Webb
    Article
    Vancraenenbroeck et al ACS Chem Biol. 2015 Nov 20;10(11):2650-7
    Insert
    matB (His6 C106A R286C Q457C K488A)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    C106A R286C Q457C K488A
    Promoter
    T7
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only

  28. RNF168-Halo HRD

    Plasmid
    #207080
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207080-plasmid-map-sequence-id-432323

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  29. FKBP_LD 0_TEV

    Plasmid
    #58877
    Purpose
    MESA protease chain with Rapamycin FKBP ectodomain and no linker domain
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA protease chain with FKBP rapamycin-binding ectodomain, TEV protease and mCherry fusion
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    58877-plasmid-map-sequence-id-138316

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  30. CyTERM-mGFP

    Plasmid
    #62237
    Purpose
    ER targeted transmembrane domain with monomeric EGFP oriented towards the cytoplasm. Used for assessing fluorescent protein oligomerization.
    Depositor
    Erik Snapp
    Article
    Costantini et al Traffic. 2012 May;13(5):643-9. doi: 10.1
    Insert
    CyTERM-mGFP
    Use
    Tags
    mGFP
    Expression
    Mammalian
    Mutation
    signal anchor of rabbit cytochrome p450 and monom…
    Promoter
    CMV
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    62237-plasmid-map-sequence-id-320621

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  38. FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual

    Plasmid
    #216377
    Purpose
    Baculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)
    Depositor
    William Weis
    Insert
    LDL receptor related protein 6 (LRP6 Human)
    Use
    Baculovirus
    Tags
    9xHis and HA signal sequence-FLAG-3C cleavage sit…
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin (LRP6)/p10 (MESD)
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216377-plasmid-map-sequence-id-433174

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  39. pVH1002-Tier2(pUC ori)

    Plasmid
    #169600
    Purpose
    Tier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and pUC ori).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    169600-plasmid-map-sequence-id-366610

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Showing: 7401 - 7450 of 9660 results