12,947 results

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pCXLE-mp53DD

Plasmid

#41859

Purpose

Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

Trp 53 (Trp53 Mouse)

Expression

Mammalian

Mutation

Δ40-903

Promoter

CAG

Available Since

July 17, 2013

Availability

Academic Institutions and Nonprofits only

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pMXs-Klf4

Plasmid

#13370

Purpose

Retroviral expression of mouse Klf4

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

Kruppel-like factor 4 (gut) (Klf4 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

Plasmid

#60224

Purpose

Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

sgRNA
Renilla luciferase
Cre recombinase
KrasG12D HDR donor (Kras Mouse)

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Tags

Cre-HA and Rluc-P2A

Expression

Mammalian

Promoter

EFS, No promoter, and U6

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-Sox2

Plasmid

#13367

Purpose

Retroviral expression of mouse Sox2

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

SRY-box containing gene 2 (Sox2 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-TNT4-Zfr-P2A-HA-YAP5SA-3xMIR122

Plasmid

#223186

Purpose

Plasmid containing the Zfr/YAP5SA/3xMIR122 cassette. Translation of YAP5SA is controlled by splicing modulation of the Zfr specifically in cardiomyocytes by risdiplam in vivo.

Depositor

Yuxuan Guo

Article

Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

Inserts

YAP5SA (Yap1 Mouse)
mir122 (Mir122 Mouse)

Use

AAV

Tags

HA and engineered P2A

Mutation

S61A, S109A, S127A, S164A, S381A

Promoter

TNNT2

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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U6REST1_U6REST2.hPGK.BRN2.hPGK.Ascl1WPRE

Plasmid

#101852

Purpose

Direct reprogramming of adult fibroblasts to neurons

Depositor

Malin Parmar

Article

Drouin-Ouellet et al EMBO Mol Med. 2017 Aug;9(8):1117-1131. d

Insert

Ascl1, Brn2

Use

Lentiviral

Promoter

U6, PGK

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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Pdx1 Cre (DM#258)

Plasmid

#15021

Depositor

Douglas Melton

Article

Gu et al Development. 2002 May . 129(10):2447-57.

Insert

Pdx1 promoter (Pdx1 Mouse)

Use

Cre/Lox; Mouse transgenic

Tags

Cre

Expression

Mammalian

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

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pABpuro-BluF

Plasmid

#46824

Purpose

luciferase reporter for BmalI promoter

Depositor

Steven Brown

Article

Brown et al PLoS Biol. 2005 Oct;3(10):e338. Epub 200

Insert

Bmal1 (Bmal1 Mouse)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Bmal1

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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pCIP-Flag-TfebAA

Plasmid

#79014

Purpose

For mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

Tfeb (Tfeb Mouse)

Tags

Flag

Expression

Mammalian

Mutation

S142A, S211A

Promoter

pCAGGs

Available Since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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Bcl11b MSCV IRES mCherry

Plasmid

#189808

Purpose

Retroviral expression of Bcl11b

Depositor

Ellen Rothenberg

Insert

Bcl11b (Bcl11b Mouse)

Use

Retroviral

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pFUGW-Pol2-ffLuc2-eGFP

Plasmid

#71394

Purpose

Lentiviral vector of luciferase-eGFP fusion gene driven by Pol2 promoter

Depositor

Glenn Merlino

Article

Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

Insert

Pol2-ffLuc2-eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

Pol2 (mouse RNA polymerase II gene promtoer)

Available Since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pT3-EF1aH NICD1

Plasmid

#86500

Purpose

Mammalian expression of Myc tagged NICD1

Depositor

Xin Chen

Article

Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

Insert

Notch1 (C-term) (Notch1 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

Cytoplasmic domain only (AA 1753-2531)

Promoter

EF1a

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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5HT6-G-GECO1.0

Plasmid

#47499

Purpose

Fluorescent reporter for Ca2+ inside primary cilia

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

5-hydroxytriptamine receptor isoform 6 (Htr6 Mouse)

Tags

G-GECO1.0

Expression

Mammalian

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pCDF-His-mTET1CD

Plasmid

#81053

Purpose

bacterial expression of the catalytic domain of murine TET1

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TET1 (Tet1 Mouse)

Tags

6HIS

Expression

Bacterial

Mutation

catalytic domain amino acid 1367-2057

Promoter

T7

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-myrAkt-P2A-BCL2

Plasmid

#233182

Purpose

Retroviral expression of myristoylated Akt1 (myr-Akt) and Bcl2

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

myr-Akt (Akt1, BCL2 Human, Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

myr

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAdCMV/V5-DEST-STAT3-3xFlag

Plasmid

#99260

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3

Depositor

Alejandro Adam

Article

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Promoter

CMV

Available Since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Tet2

Plasmid

#60939

Purpose

Expresses Tet2 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Ten-Eleven Translocation 2 (Tet2 Mouse)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pAdCMV/V5-DEST-Y705F-STAT3-3xFlag

Plasmid

#99261

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3 (Y705F)

Depositor

Alejandro Adam

Article

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 - Y705F (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Mutation

Y705F (contains a silent mutation: nucleotide 271…

Promoter

CMV

Available Since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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TUBB5-Halo

Plasmid

#64691

Purpose

Expresses beta-tubulin labeled with Halo-tag

Depositor

Yasushi Okada

Article

Uno et al Nat Chem. 2014 Aug;6(8):681-9. doi: 10.1

Insert

TUBB

Tags

Halo-tag

Expression

Mammalian

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pLVX-hSyn-Galpha-q ONE-GO

Plasmid

#204336

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-q ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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TRE-UBC-mCD47-T2A-GFP

Plasmid

#205449

Purpose

lentiviral plasmid for expression of mouse CD47

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mCD47 (Cd47 Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Mm.cargo(Cre)

Plasmid

#174862

Purpose

Expresses mouse Peg10 cargoRNA encoding Cre recombinase

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

Cre flanked by MmPeg10 UTRs

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTubulin-miRFP670-2

Plasmid

#197238

Purpose

Expresses the fusion protein of Tubulin-miRFP670-2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Tubulin-miRFP670-2

Expression

Mammalian

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Prdm9CD

Plasmid

#235577

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Prdm9 fused with scFV to programme H3K4me3

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Prdm9 (Prdm9 Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G

Plasmid

#194458

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

Depositor

Ana Sofia Coroadinha

Article

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-CD19 CAR

Use

Lentiviral

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

Plasmid

#67977

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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mEmerald-Integrin-Beta3-N-18

Plasmid

#54130

Purpose

Localization: Integrin/Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Integrin-Beta3

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pMXs-c-Myc

Plasmid

#13375

Purpose

Retroviral expression of mouse c-Myc

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

myelocytomatosis oncogene (Myc Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
murine TCR OTII-2A.pMIG II

Plasmid

#52112

Purpose

retroviral expression of both TCRalpha and TCRbeta using a single vector

Depositor

Dario Vignali

Article

Holst et al Nat Protoc. 2006;1(1):406-17.

Inserts

T cell receptor alpha chain (Tcra Mouse)
T cell receptor beta chain (Tcrb Mouse)

Use

Retroviral

Tags

P2A (from porcine teschovirus-1)

Expression

Mammalian

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122)

Plasmid

#209079

Purpose

Ngn2 mediated cortical neuron induction, along with sfGFP over-expression

Depositor

Vikram Khurana

Article

Lam et al bioRxiv 2022.11.08.515615

Inserts

sfGFP
NGN2 (Neurog2 Mouse)

Expression

Mammalian

Promoter

TRE4G

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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