We narrowed to 13,035 results for: SHI

1. pBAD/His-miRFP718nano

   Plasmid

   #195743

   Purpose

   Monomeric near-infrared fluorescent protein miRFP718nano in bacterial plasmid

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

   Insert

   miRFP718nano
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   araC

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGas-miRFP718nano

   Plasmid

   #195752

   Purpose

   G protein alpha-subunit internally tagged with miRFP718nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

   Insert

   G protein alpha-s-miRFP718nano
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGWT35S-VC155

   Plasmid

   #194051

   Purpose

   Transient expression of VN155 (mVenus β-strands 8 to 11, amino acids 155−239) in plant cell (Cytosol)

   Depositor

   Yutaka Kodama

   Article

   Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

   Insert

   VC155 (mVenus β-strands 8 to 11, amino acids 155−239)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S promoter

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGWT35S-AtOEP7(1-50aa)-mVenus

   Plasmid

   #194000

   Purpose

   Transient expression of AtOEP7-mVenus in plant cell (Chloroplast outer envelope membrane)

   Depositor

   Yutaka Kodama

   Article

   Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

   Insert

   mVenus
   

   Use

   Tags

   AtOEP7 1–50aa

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S promoter

   Available since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGWT35S-VN154

   Plasmid

   #194050

   Purpose

   Transient expression of VN154 (mVenus β-strands 1 to 7, amino acids 1−154) in plant cell (Cytosol)

   Depositor

   Yutaka Kodama

   Article

   Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

   Insert

   VN154 (mVenus β-strands 1 to 7, amino acids 1−154)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S promoter

   Available since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-PLS-KP7_rpoA-U

   Plasmid

   #190974

   Purpose

   Test the U-to-C editing activity of KP7 on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP7
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-PLS-KP2-E67A_rpoA-U

   Plasmid

   #190959

   Purpose

   Test the U-to-C editing activity of KP2:E67A on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP2
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   DYW-KP:E67A

   Promoter

   T7

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-PLS-KP6-A66S_rpoA-C

   Plasmid

   #190968

   Purpose

   Test the C-to-U editing activity of KP6:A66S on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP6
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   DYW-KP:A66S

   Promoter

   T7

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PM18033-PLS-KP5_rpoA-U

   Plasmid

   #190985

   Purpose

   Test the U-to-C editing activity of KP5 on AtrpoA editing site, in human cells

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP5
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-PLS-KP1_rpoA-U

    Plasmid

    #190955

    Purpose

    Test the U-to-C editing activity of KP1 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP1
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-PLS-KP2-A66S_rpoA-U

    Plasmid

    #190957

    Purpose

    Test the U-to-C editing activity of KP2:A66S on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:A66S

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-PLS-KP3-A66S_rpoA-U

    Plasmid

    #190961

    Purpose

    Test the U-to-C editing activity of KP3:A66S on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:A66S

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PM18033-PLS-KP7_rpoA-U

    Plasmid

    #190994

    Purpose

    Test the U-to-C editing activity of KP7 on AtrpoA editing site, in human cells

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP7
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET-PLS-KP3-E67A_rpoA-U

    Plasmid

    #190963

    Purpose

    Test the U-to-C editing activity of KP3:E67A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:E67A

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-PLS-KP4_rpoA-U

    Plasmid

    #190964

    Purpose

    Test the U-to-C editing activity of KP4 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP4
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-PLS-KP6-E67A_rpoA-C

    Plasmid

    #190972

    Purpose

    Test the C-to-U editing activity of KP6:E67A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:E67A

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PM18033-PLS-KP4_rpoA-U

    Plasmid

    #190984

    Purpose

    Test the U-to-C editing activity of KP4 on AtrpoA editing site, in human cells

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP4
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-PLS-KP5_rpoA-U

    Plasmid

    #190965

    Purpose

    Test the U-to-C editing activity of KP5 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP5
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAFMW-Nbr-CM

    Plasmid

    #188585

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler Catalytic mutant
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    deleted first ATG; D435A [GAT=>GCC]; E435A [GA…

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAFMW-Nbr[N1-383]

    Plasmid

    #188586

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler N-terminal domain
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    deleted first ATG

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAFMW-Nbr[C399-625]

    Plasmid

    #188587

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler C-terminal domain
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. InterTag-1xL

    Plasmid

    #186911

    Purpose

    Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a single GGGGS linker and the other side expressing BFP and puromycin resistance gene

    Depositor

    Taekjip Ha, Allen Liu

    Article

    Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

    Insert

    SpyTag003-sfCherry11-(GGGGS)1-PDGFR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. L1-AA027

    Plasmid

    #185752

    Purpose

    Level 1 loop assembly vector containing a transcriptional unit with a Hygromycin resistance cassette under the control of the A. agrestis EF1a promoter (pAaEF1a).

    Depositor

    Peter Szoevenyi

    Article

    Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

    Insert

    HygR2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEGFP.N3-myc-SLC35A2

    Plasmid

    #186285

    Purpose

    transient expression of N-terminal myc tagged SLC35A2 isoform c/UGT1 in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Li et al Glycobiology. 2019 Jun 1;29(6):490-503.

    Insert

    SLC35A2 (SLC35A2 Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. gSCPL35-mRFP

    Plasmid

    #181960

    Purpose

    SCPL35 protein tagged with mRFP, under control of native promoter

    Depositor

    George Haughn

    Article

    Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

    Insert

    SCPL35
    

    Use

    Tags

    mRFP

    Expression

    Plant

    Mutation

    Promoter

    native SCPL35

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAc5.1B-lambdaN-HA-DmHPat_57-499_B

    Plasmid

    #145979

    Purpose

    Insect Expression of DmHPat_57-499

    Depositor

    Elisa Izaurralde

    Article

    Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

    Insert

    DmHPat_57-499 (Patr-1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    two silent mutations compared to the sequence giv…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAc5.1B-EGFP-DmMe31B_1-267_D

    Plasmid

    #146118

    Purpose

    Insect Expression of DmMe31B_1-267

    Depositor

    Elisa Izaurralde

    Article

    Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

    Insert

    DmMe31B_1-267 (me31B Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAc5.1B-EGFP-DmHPat_1-225_A

    Plasmid

    #145870

    Purpose

    Insect Expression of DmHPat_1-225

    Depositor

    Elisa Izaurralde

    Article

    Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

    Insert

    DmHPat_1-225 (Patr-1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one non silent mutation N218K compared to the seq…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAc5.1B-EGFP-DmHPat_500-968_A

    Plasmid

    #145873

    Purpose

    Insect Expression of DmHPat_500-968

    Depositor

    Elisa Izaurralde

    Article

    Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

    Insert

    DmHPat_500-968 (Patr-1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A1758G compared to the sequen…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAc5.1B-lambdaN-HA-DmHPat_1-225_A

    Plasmid

    #145875

    Purpose

    Insect Expression of DmHPat_1-225

    Depositor

    Elisa Izaurralde

    Article

    Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

    Insert

    DmHPat_1-225 (Patr-1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one non silent mutation N218K compared to the seq…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAc5.1B-lambdaN-HA-DmHPat_1-499_A

    Plasmid

    #145876

    Purpose

    Insect Expression of DmHPat_1-499

    Depositor

    Elisa Izaurralde

    Article

    Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

    Insert

    DmHPat_1-499 (Patr-1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    two silent mutations compared to the sequence giv…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAc5.1B-lambdaN-HA-DmXRN1_A

    Plasmid

    #145879

    Purpose

    Insect Expression of DmXRN1

    Depositor

    Elisa Izaurralde

    Article

    Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

    Insert

    DmXRN1 (pcm Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCS2+-wbp2nlY282F

    Plasmid

    #172468

    Purpose

    expresses protein with one aa substitution to affect potential phosphorylation in WW-interaction domain

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nlY282F
    

    Use

    Xenopus expression

    Tags

    none

    Expression

    Mutation

    changed Tyrosine at aa282 to Phenylalanine

    Promoter

    SP6

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. tcTRP24sub8_Cap

    Plasmid

    #175797

    Purpose

    de novo thick circular tandem repeat protein capped to prevent multimerization

    Depositor

    Barry Stoddard

    Article

    Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

    Insert

    tcTRP24sub8_Cap
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. SH2-free

    Plasmid

    #175800

    Purpose

    Human Nck2 SH2 domain

    Depositor

    Barry Stoddard

    Article

    Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

    Insert

    human Nck2 SH2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. tcTRP9

    Plasmid

    #175793

    Purpose

    de novo thick circular tandem repeat proteins with 9 repeats

    Depositor

    Barry Stoddard

    Article

    Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

    Insert

    tcTRP9
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCS2+-wbp2nlF127P

    Plasmid

    #174507

    Purpose

    expresses protein with one aa substitution to disrupt the alpha-helix near the PH-G domain

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nlF127P
    

    Use

    Xenopus expression

    Tags

    none

    Expression

    Mutation

    changed Phenylalanine at aa127 to Proline

    Promoter

    SP6

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCS2+-wbp2nlT45A

    Plasmid

    #172464

    Purpose

    expresses protein with one aa substitution to affect potential phosphorylation

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nlT45A
    

    Use

    Xenopus expression

    Tags

    none

    Expression

    Mutation

    changed Threonine at aa45 to Alanine

    Promoter

    SP6

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCS2+-wbp2nlY55F

    Plasmid

    #172466

    Purpose

    expresses protein with one aa substitution to affect potential phosphorylation in ITSM domain

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nlY55F
    

    Use

    Xenopus expression

    Tags

    none

    Expression

    Mutation

    changed Tyrosine at aa55 to Phenylalanine

    Promoter

    SP6

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pWSK129-stm2585-gp130

    Plasmid

    #174404

    Purpose

    Bacterial expression of sarA-gp130 chimera under native promoter.

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    sarA-IL6ST chimera
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Endogenous

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. Negative feedback (NF) plasmid

    Plasmid

    #169747

    Purpose

    Standalone NF circuit plasmid that has the same implementation of the NF subcircuit in Equalizers.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    tetR-P2A-eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tetO2

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pAc5.1B-EGFP-DmMe31B_268-459_D

    Plasmid

    #146119

    Purpose

    Insect Expression of DmMe31B_268-459

    Depositor

    Elisa Izaurralde

    Article

    Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

    Insert

    DmMe31B_268-459 (me31B Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pMRBad-C-DiB-RM

    Plasmid

    #168478

    Purpose

    C-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168476: pET11a-N-DiB-RM to obtain DiB-RM‐split protein

    Depositor

    Jens Meiler

    Article

    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

    Insert

    C-fragment of DiB-RM
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET11a-N-DiB-RM

    Plasmid

    #168476

    Purpose

    N-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168478: pMRBad-C-DiB-RM to obtain DiB-RM‐split protein

    Depositor

    Jens Meiler

    Article

    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

    Insert

    N-fragment of DiB-RM
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pET11a-Z-N-DiB2

    Plasmid

    #168473

    Purpose

    N-fragment of the DiB2‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain DiB2‐split-Zip protein

    Depositor

    Jens Meiler

    Article

    Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10

    Insert

    N-fragment of DiB2 + Leucine Zipper
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. PB-TAC-Cas9

    Plasmid

    #120352

    Purpose

    piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on and CBh

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. PB-TAC-KRAB-dCas9

    Plasmid

    #120355

    Purpose

    piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on and CBh

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCFJ2365 - Intron GG1 - 250bp no PATC

    Plasmid

    #159883

    Purpose

    Golden Gate compatible intron with no PATCs for negative controls

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron GG1 - 250bp no PATC
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCFJ2345 - Intron GG2 - 250bp no PATC

    Plasmid

    #159884

    Purpose

    Golden Gate compatible intron with no PATCs for negative controls

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron GG2 - 250bp no PATC
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCFJ2366 - intron GG3 - 250bp no PATC

    Plasmid

    #159885

    Purpose

    Golden Gate compatible intron with no PATCs for negative controls

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    intron GG3 - 250bp no PATC
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge