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Showing: 7501 - 7550 of 21593 results

  17. pHEA

    Plasmid
    #168297
    Purpose
    Bacterial surface display of polypeptides (nanobodies) in E. coli
    Depositor
    Luis Ángel Fernández
    Article
    Marin et al J Bacteriol. 2010 Nov;192(21):5588-602.
    Insert
    Enterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
    Use
    Tags
    6xHis, E-tag, and PelB signal peptide
    Expression
    Bacterial
    Mutation
    Promoter
    LacI-Plac
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    168297-plasmid-map-sequence-id-334448

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  26. pTJV1ScKm-rpoB

    Plasmid
    #166980
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Insert
    NeoR/KanR
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166980-plasmid-map-sequence-id-331333

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  29. T444T-GFP(NCO)

    Plasmid
    #183972
    Purpose
    Enhanced RNAi clone for C. elegans knockdown of non-codon-optimized GFP
    Depositor
    David Matus
    Article
    Palmisano et al CourseSource, 2022
    Insert
    GFP (non-codon-optimized)
    Use
    RNAi
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    183972-plasmid-map-sequence-id-364073

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  32. EB3-mScarlet-I

    Plasmid
    #98826
    Purpose
    In vivo visualization of microtuble plus ends (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    EB3
    Use
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    98826-plasmid-map-sequence-id-190905

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  39. pGWB701NL3F10H

    Plasmid
    #141288
    Purpose
    No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, Tunicamycin R for plant)
    Depositor
    Andrew Millar
    Article
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Type
    Empty backbone
    Use
    Luciferase; Gateway
    Tags
    FLAG (x3), HIS (x10), and NLUC
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    141288-plasmid-map-sequence-id-324889

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  49. pMito-TrxRFP1

    Plasmid
    #98997
    Purpose
    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, mitochondrially locaclized
    Depositor
    Huiwang Ai
    Article
    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038
    Insert
    TrxRFP1 and mitochondrial localization sequence
    Use
    Tags
    His-tag and Mitochondria localization sequence
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    98997-plasmid-map-sequence-id-189692

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  50. ITPKA-mNeonGreen

    Plasmid
    #98883
    Purpose
    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    ITPKA
    Use
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    98883-plasmid-map-sequence-id-191169

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Showing: 7501 - 7550 of 21593 results