We narrowed to 10,133 results for: gnas
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Plasmid#42234DepositorInsertCas9
UseCRISPRTagsGFP, HA epitope, and NLS (nuclear localization si…ExpressionMammalianMutationcodon-optimized synthetic DNA sequencePromoterCMVAvailable SinceMarch 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET24a isPETase
Plasmid#225759PurposeExpresses codon-optimized PETase from Ideonella sakaiensis in E. coli without native signal sequence and with a C-terminal his-tagDepositorInsertisPETase
Tags6xHis TagExpressionBacterialPromoterT7Available SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSR33.4r
Plasmid#63198PurposeExpresses Cph8, Ho1, and PcyA constitutivelyDepositorInsertsCph8
Ho1
PcyA
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
Beta-Lactamase
Plasmid#62729PurposeExpresses Beta-Lactamase protein in bacterial cellsDepositorInsertBeta-Lactamase
TagsHis tagExpressionBacterialMutationDeletion of periplasmic signal domainPromoterT7Available SinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMV306hsp+Lux
Plasmid#26159DepositorInsertBacterial luciferase operon
ExpressionBacterialMutationIt contains a gram-positive enhanced translation …Available SinceFeb. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSR58.6
Plasmid#63176PurposeExpresses CcaR constitutively and sfGFP under the PcpcG2-172 promoterDepositorInsertsCcaR
sfGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
ERmoxGFP
Plasmid#68072Purposemammalian expression of ER localized moxGFPDepositorInsertmoxGFP
TagsKDEL ER retention sequence and bovine prolactin s…ExpressionMammalianPromoterCMVAvailable SinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-mCherry-NLS
Plasmid#194049PurposeTransient expression of mCherry-NLS in plant cell (Nucleus)DepositorInsertmCherry
TagsSV40 (Nuclear localization signal)ExpressionPlantPromoterCaMV 35S promoterAvailable SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPhlF-BCD22-sfGFP-ssrA P64U26C39T11 v1-1 (632)
Plasmid#70742Purpose1 color weak reporter used in studyDepositorInsertsfGFP-ssrA
Available SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MTS-jAspSnFR3-mRuby3
Plasmid#203492PurposeMitochondrial directed jAspSnFR3-mRuby3 in lentiviral expression vectorDepositorInsertMTS-jAspSnFR3-mRuby3
UseLentiviralTagsHis-tag, Mitochondrial localization signal, and m…ExpressionMammalianPromoterCMVAvailable SinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-GFP1-10-HygR
Plasmid#223675PurposeTo check the split GFP signals in mitochondria (1)DepositorInsertGFP1-10
ExpressionMammalianPromotercmvAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-3C-sGFP11_pcDNA3.1(-)
Plasmid#182932PurposeFor expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragmentDepositorTypeEmpty backboneTags3C-cleavable split GFP 11, FLAG tag, and HA signa…ExpressionMammalianPromoterCMVAvailable SinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSR43.6r
Plasmid#63197PurposeExpresses CcaS, Ho1, and PcyA constitutivelyDepositorInsertsCcaS
Ho1
PcyA
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAV0765
Plasmid#133501PurposeExpression of mCherry targeted to the nucleus with one NLS signalsDepositorInsertpLys3(BstZ17I)-p(tdh1)-NLSSV40-mCherry-terminator(ScADH1)-kanMX
ExpressionYeastAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-MYC
Plasmid#86461PurposeA modified version of the Promega pGL4.23 vector. Contains the MYC promoter in place of the minimal promoter and polyadenylation signals flanking the enhancer cloning site.DepositorTypeEmpty backboneUseLuciferaseExpressionMammalianPromoterMYCAvailable SinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_ssSBP-EGFP
Plasmid#65277Purposeto synchronize transport of secretory soluble GFP from the ER (RUSH system)DepositorInsertEGFP
TagsIL-2 signal sequence and Streptavidin Binding Pr…ExpressionMammalianPromoterCMVAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Ani- α Amylase SP
Plasmid#153503PurposeAspergillus niger α Amylase Signal Peptide Donor PlasmidDepositorInsertα Amylase SP
UseSynthetic BiologyAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYC1446
Plasmid#158720PurposeIntegrating plasmid expressing Sth1 Cas9 and the cognate sgRNADepositorInsertSth1 sgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEVA63_HelperΔgIII
Plasmid#190433PurposeM13 helper genes cloned into the pSEVA631 vector, except the minor coat protein coding gene gIII (also called gp3, or g3p). This plasmid does not carry an M13 packaging signal.DepositorInsertM13 genes I-V and VII-XI
UseSynthetic BiologyAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEVA63_Helper
Plasmid#190432PurposeM13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ297.)DepositorInsertM13 genes: I-XI
UseSynthetic BiologyAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYES2-Ub-R-GFP
Plasmid#11953DepositorInsertUb-R-GFP
ExpressionYeastMutationUbiquitin fused to N-terminus of GFP. Arginine at…Available SinceMay 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
tTA_BFP
Plasmid#58854PurposeConstitutive expression of tTA-EBFP fusion protein (tTA from CL-CTIG, Addgene plasmid 14901)DepositorInserttTA transcription factor fused to enhanced blue fluorescent protein
TagsBFPExpressionMammalianPromoterCMVAvailable SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMIII-Neo
Plasmid#153422PurposeMSCV with IRES-Neomycin resistant geneDepositorTypeEmpty backboneUseRetroviralExpressionMammalianAvailable SinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lyn-R-GECO1
Plasmid#120410PurposeExpresses a fusion between myristoylation signal peptide and R-GECO1. This construct targets R-GECO1 to the plasma membrane.DepositorInsertLyn-R-GECO1
ExpressionMammalianPromoterCMVAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-PTS1-IRES-Puromycin
Plasmid#134665PurposeExpresses EGFP-tagged peroxisome markerDepositorInserttripeptide SKL (Peroxisome targeting signal 1)
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1aAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJKR-O-mphR
Plasmid#62570PurposeMacrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids.DepositorInsertmCherry
ExpressionBacterialAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
SOX2se-CPG-TV
Plasmid#70155Purposea targeting vector to target the endogenous Sox2 SE CPG island with Snrp-Tomato-polyADepositorInsert5'hSOX2se-Snrp-Tomato-polyA-Pgk-PURO-3'hSOX2se
UseMouse Targeting and Synthetic BiologyAvailable SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET22b - PA
Plasmid#84863Purposebacterial expression of Bacillus anthracis protective antigenDepositorInsertPA
TagspelB signal sequenceExpressionBacterialPromoterT7Available SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_SBP-EGFP-Ecadherin
Plasmid#65288Purposesynchronize trafficking of Ecadherin from the ER (RUSH system)DepositorInsertEcadherin
TagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…ExpressionMammalianPromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-H2-18 Light chain
Plasmid#216308PurposeExpresses anti-HER2 H2-18 Light chain in mammalian cells. To be paired with H2-18 heavy chain to form the anti-HER2 H2-18 FabDepositorInsertH2-18 Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a MDHP_YEAST
Plasmid#204233PurposeBacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHP_YEAST (MDH3 Budding Yeast)
Tags6X HisExpressionBacterialMutationThe last 3 C-terminal residues (SKL) are the pero…PromoterLacAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal
Plasmid#52694PurposeThis plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.DepositorInsertsCas9
Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
UseCRISPR; Toxoplasma gondiiTags3X FLAG and Nuclear localization signalPromoterTgTUB1 and Toxoplasma U6Available SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-GLP1R Fusion Vector
Plasmid#238559PurposeExpress HiBiT-GLP1R Fusion Protein in Mammalian Cells under a CMV promoterDepositorHas ServiceDNAInsertGLP1R (GLP1R Human)
TagsHiBiTExpressionMammalianMutationDomain 24-463 (native signal peptide deleted)PromoterCMVAvailable SinceOct. 29, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ii-Str_SBP-mCherry-Ecadherin
Plasmid#65289Purposesynchronize trafficking of Ecadherin from the ER (RUSH system)DepositorInsertEcadherin
TagsIL-2 Signal Sequence and Streptavidin Binding Pro…ExpressionMammalianPromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBI-MCS-EYFP
Plasmid#58855PurposeThe tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFPDepositorInsertenhanced yellow fluorescent protein
ExpressionMammalianPromoterCMVAvailable SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
FLAG-3C-sGFP1-10_pcDNA3.1(-)
Plasmid#182931PurposeFor expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragmentDepositorTypeEmpty backboneTags3C-cleavable split GFP 1-10, FLAG tag, and HA sig…ExpressionMammalianPromoterCMVAvailable SinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIBA2-Int-SpyTag
Plasmid#198038PurposeExpression plasmid for producing SpyTagged stalled secretion variant of intiminDepositorInserteaeA
TagsSpyTagExpressionBacterialMutationlacks native signal peptide (amino acids 1-39)PromotertetAvailable SinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNR291
Plasmid#79555PurposeOutput plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.DepositorInsertsGFPmut3b
mTagBFP
mRFP
ExpressionBacterialAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTetR-BCD20-mCherry-ssrA P66U23C90T20 v40-3a (588)
Plasmid#70745Purposefor building 3-color reporter plasmidDepositorInsertmCherry-ssrA
Available SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
ERoxBFP
Plasmid#68126Purposemammalian expression of ER localized oxBFPDepositorInsertoxBFP
TagsKDEL ER retention sequence and bovine prolactin s…ExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_SBP-EGFP-GPI
Plasmid#65296Purposesynchronize trafficking of EGFP-GPI from the ER (RUSH system)DepositorInsertGPI anchor
TagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…ExpressionMammalianPromoterCMVAvailable SinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-F0ATPMLS-mCherry-GFP1-10
Plasmid#211466PurposeLentivirus backbone expressing mCherry and GFP1-10 fusion protein with a mitochondrial localization signalDepositorInsertmito-mCherry-GFP1-10
UseLentiviralExpressionMammalianPromoterEF-1aAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAPV-DsRed2-ER-5
Plasmid#170570PurposeExpresses DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after signal peptide cleavage.DepositorInsertAPV-DsRed2-ER-5
ExpressionMammalianPromoterCMVAvailable SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_PsChR2_EYFP
Plasmid#69057PurposeHigh-efficiency blue-shifted cation channelrhodopsinDepositorInsertPsChR2
TagsEYFPExpressionMammalianPromoterCMVAvailable SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJKR-H-cdaR
Plasmid#62557PurposeGlucarate biosensor. High copy plasmid that expresses GFP proportional to glucarate concentration.DepositorInsertsbeta lactamase
sfGFP
CdaR
ExpressionBacterialAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHEA
Plasmid#168297PurposeBacterial surface display of polypeptides (nanobodies) in E. coliDepositorInsertEnterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
Tags6xHis, E-tag, and PelB signal peptideExpressionBacterialPromoterLacI-PlacAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-jGCaMP7b-XN
Plasmid#178362PurposeNuclear localized GCaMP-X sensor for monitoring calcium signal in nucleus.DepositorInsertjGCaMP7b-XN
ExpressionMammalianAvailable SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
GeNL(Ca2+)_480/pcDNA3
Plasmid#85205Purposeluminescent reporter for calcium signalingDepositorInsertGeNL(Ca2+)_480
ExpressionMammalianPromoterCMVAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
dTomato_LD 0_TEV
Plasmid#58872PurposeMESA protease chain with dTomato ectodomain and no linker domainDepositorInsertMESA protease chain with dTomato ectodomain and TEV protease
UseAAVExpressionMammalianPromoterCMVAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHis17_ParM _1
Plasmid#78201PurposeExpression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensorDepositorInsertparM (K33A I27C T174A T175N C287A)
TagsHis6ExpressionBacterialMutationK33A I27C T174A T175N C287APromoterunknownAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only