8,885 results
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pBABE puro mouse IRS-1 myc
Plasmid#11374DepositorAvailable SinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Zeo-gfpmut3
Plasmid#65037PurposeZeoR mini-Tn7 vector, mobilizable, for GFP tagging bacteriaDepositorInsertgfpmut3
ExpressionBacterialAvailable SinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGFP FAK
Plasmid#50515PurposeThis plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertprotein tyrosine kinase
TagsGFPExpressionMammalianPromoterCMVAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_TNF-SBP-mCherry
Plasmid#65279Purposesynchronize trafficking of TNF from the ER (RUSH system)DepositorInsertTNF (TNF Human)
TagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianPromoterCMVAvailable SinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
hATMS1981A
Plasmid#32300DepositorAvailable SinceNov. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
AcGFP1-N1
Plasmid#54705PurposeLocalization: N1 Cloning Vector, Excitation: 475, Emission: 505DepositorTypeEmpty backboneTagsAcGFP1ExpressionMammalianAvailable SinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Rab5a-7
Plasmid#54243PurposeLocalization: Ras GTPase, Excitation: 487, Emission: 509DepositorInsertRab5a
TagsmEmeraldExpressionMammalianPromoterCMVAvailable SinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBABE-puro TPR-Met
Plasmid#10902DepositorUseRetroviralExpressionMammalianMutationConstitutively active form of the c-Met receptorAvailable SinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 HA-TBRII (delta Cyt)
Plasmid#14051DepositorInsertTGF beta rec II (delta cyt) (TGFBR2 Human)
TagsHA (PYDVPDYA)ExpressionMammalianMutationDominant negative mutant. A stop codon was introd…Available SinceMay 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
Lac-I-SceI-Tet
Plasmid#17655DepositorInsertlac I-SceI tet
ExpressionBacterialAvailable SinceMay 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBUE411
Plasmid#62200PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistanceDepositorInsertsgRNA scaffold
zCas9
UsePlant binary vectorTags3x FLAG and NLSExpressionPlantMutationmaize codon optimizedPromoterOsU3 and UbiAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABE-zeo largeTgenomic
Plasmid#1778DepositorAvailable SinceJune 23, 2005AvailabilityAcademic Institutions and Nonprofits only -
pARF6-CFP
Plasmid#11382DepositorAvailable SinceMarch 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTRE_GFP-GluR1
Plasmid#34857DepositorAvailable SinceFeb. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRubiG-T2A-Cas9
Plasmid#75348PurposeUbiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.DepositorInsertCas9
UseCRISPR and RetroviralTagsGFP (via t2a)ExpressionMammalianPromoterUbiquitinAvailable SinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAX APP delta CT
Plasmid#30143DepositorInsertAmyloid Precursor Protein (APP Human)
ExpressionMammalianMutationdeletion of C-terminal intracellular domain (afte…Available SinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPUR-hU6-sgRNA-Sirius-8XMS2
Plasmid#121942PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XMS2
UseCRISPR and LentiviralTagsnoExpressionMammalianPromoterhuman U6 promoterAvailable SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-3xMBT
Plasmid#46987PurposeInducible expression of 3xMBT from L3MBTL1 as a GST fusion protein in E.coliDepositorInsertTriple MBT domain from human L3MBTL1 (L3MBTL1 Human)
TagsGST and PreScission cleavage siteExpressionBacterialMutationcontains amino acids 190–530 of accession NP_0562…PromotertacAvailable SinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro-IMS-RP
Plasmid#24535DepositorInsertDiablo homolog (DIABLO Human)
UseRetroviralTagsmRFP1ExpressionMammalianMutationDeleted residues 56-239Available SinceJune 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
dxCas(3.7)-VPR
Plasmid#108383PurposeMammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminusDepositorInsertdxCas(3.7)-VPR
TagsNLSExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VAvailable SinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2A
Plasmid#15780DepositorAvailable SinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CamKII 0.4.Cre.SV40
Plasmid#105558PurposeAAV expression of Cre from CamKII promoterDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertCre
UseAAV and Cre/LoxExpressionMammalianPromoterCamKIIAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKH3-human RSK1
Plasmid#13841DepositorAvailable SinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSlow-FT-N1
Plasmid#31912DepositorInsertSlow-FT
ExpressionMammalianMutationM18V/E30V/K69R/L84W/A179V compared to mCherry (bu…PromoterCMVAvailable SinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDG461
Plasmid#100902PurposeSpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.DepositorInsertshumanized CRISPR associated protein 9 Nickase
U6-gRNA scaffold 1
U6-gRNA scaffold 2
UseCRISPR and Mouse TargetingTags3xFLAG and T2A-EGFPExpressionMammalianMutationD10A mutant converts to NickasePromoterCBh and U6Available SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-nsp1-2xStrep-IRES-Puro
Plasmid#141367PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp1 (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable SinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Myc-PSF-WT
Plasmid#35183DepositorAvailable SinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pQE60-RRF-His
Plasmid#124132PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-ABERA-P2A-Puro
Plasmid#112675PurposeLentivirus for constitutive expression of ABE7.10 RA (codon optimized)DepositorInsertABERA
UseLentiviralTagsFLAGMutationD10APromoterEF1sAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI-SEP-NRI
Plasmid#23999DepositorInsertNR1 (Grin1 Rat)
TagsNR1 signal sequence (aa1-20) and SEP (supereclipt…ExpressionMammalianPromoterCMVAvailable SinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro LEF1
Plasmid#27023DepositorAvailable SinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
PU6::unc-119_sgRNA
Plasmid#46169Purposeunc-119 targeting sgRNADepositorInsertunc-119 targeting sgRNA (unc-119 Synthetic)
UseCRISPRPromoterC. elegans U6 snRNA pol III promoterAvailable SinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCFJ151 - ttTi5605_MCS
Plasmid#19330DepositorArticleInsertttTi5605 targeting region
ExpressionWormAvailable SinceOct. 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
mOrange-N1
Plasmid#54499PurposeLocalization: N1 Cloning Vector, Excitation: 548, Emission: 562DepositorTypeEmpty backboneTagsmOrangeExpressionMammalianAvailable SinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET32a-TRXtag
Plasmid#11516DepositorInsertNone - thioredoxin is vector encoded (trxA )
TagsHis, His (out of frame), and TrxExpressionBacterialMutationvector modified; removed N-terminal His tag by di…Available SinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TDsmURFP-IRES-HO-1
Plasmid#80346PurposeExpresses Tandem Dimer smURFP and Heme Oxygenase-1 in mammalian cells.DepositorInsertsTandem Dimer smURFP
HO-1
ExpressionMammalianPromoterCMV and IRESAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/V5-His Snk/Plk2
Plasmid#16015DepositorAvailable SinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSicoR
Plasmid#11579PurposeCre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF shRNA expression.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiExpressionMammalianAvailable SinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP21
Plasmid#22574DepositorAvailable SinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only
