We narrowed to 17,271 results for: form
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Plasmid#108244PurposeMembrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expressionDepositorInsertMembrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker
UseSynthetic BiologyPromoterpLacO1Available SinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-(mcs) human CSF1 full length-IRES-Puro
Plasmid#86797Purposeretroviral expression of CSF-1DepositorAvailable SinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
wt Opa1
Plasmid#204427PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 protein.DepositorAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB405
Plasmid#74799PurposeGateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5 alpha6
Plasmid#16036DepositorInsertalpha6 (ITGA6 Human)
ExpressionMammalianAvailable SinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag-eGFP-dspB(E184Q W330Y)-6xHis
Plasmid#175801PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
TagsAviTag, Hexahistidine tag, and eGFPExpressionBacterialMutationE184Q W330YPromoterT7Available SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.4 human alpha4 full
Plasmid#221397PurposeMammalian expression of human integrin alpha4 full lengthDepositorAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag p38 beta2
Plasmid#20355DepositorAvailable SinceMay 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-mCherry-FRB
Plasmid#186576PurposeExpression of mCherry-FRB at the outer surface of the lysosome using LAMP1DepositorAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCashGem-Xyl2-URA3-LINEAR
Plasmid#174840PurposepRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitisDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastPromoterENO1pAvailable SinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i3-IRESWT-Gbetagamma ONE-GO
Plasmid#204341PurposeGPCR/G protein BRET biosensorDepositorInsertGalpha-i3/Gbetagamma ONE-GO
UseLentiviralAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSLQ4419 pHR (hU6-BsmBI-BsmBI-EFS-PuroR-WPRE)
Plasmid#214875PurposeEmpty lentiviral backbone for guide array cloningDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_AIO-TetOn_Down-Tandem Donor
Plasmid#229785PurposeBxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)DepositorInsertLacZ; rtTA-T2A-mTagBFP2
UseSynthetic BiologyExpressionMammalianPromoterTRE and CAGAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTH727-CEN-RLuc/staCFLuc
Plasmid#38211DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationThe last three codons of the full-length Firefly …PromoterADH1 and TDH3 (=GPD)Available SinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i1 ONE-GO
Plasmid#189735PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHJ4: LexA-VHH-Caffeine
Plasmid#108242PurposeLexA DNA binding domain fusing with VHH antibody which can be dimerized by ligand caffeine. Upon ligand binding, LexA-VHH-Caffeine can repress down stream reporter gene sfGFP expression.DepositorInsertLexA DNA binding domain fusing with VHH-Caffeine antibody
UseSynthetic BiologyPromoterpLacO1Available SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K29R
Plasmid#17602PurposeMammalian expression of HA tagged ubiquitin K29RDepositorAvailable SinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-mGFP-FMR1iso1-CC2mut (V427P)
Plasmid#225466PurposeExpress mEGFP-fusion protein of FMR1 isoform 1 (V427P)DepositorAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
σ2-mCherry
Plasmid#186579PurposeExpression of adaptor protein 2 (AP2) sigma subunit AP2S1 tagged with mCherryDepositorAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-LMP2A-IRES-GFP
Plasmid#220352PurposeExpresses EBV latent gene LMP2A in mammalian cellsDepositorAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3alpha2_35S:P19:Tnos (GB1203)
Plasmid#68214PurposeTranscriptional unit for Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression driven by the 35S promoterDepositorInsertP19 silencing suppressor
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp MCP-mCherry IRES h2b-diRFP
Plasmid#102351PurposeLentiral expression vector bearing the MS2 Coat Protein (MCP) fused to mCherry as well as the nuclear marker histone 2b fused to two copies of irFP. Also bears the hygromycin selection marker.DepositorInsertMS2 Coat Protein
UseLentiviralTagsmCherryPromoterSFFVAvailable SinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND S297A
Plasmid#169966PurposepDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon)DepositorAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
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IL2 pMalLP
Plasmid#41808DepositorInsertIL2 (IL2 Human)
Tags3C protease site, 6x His, and Maltose Binding Pro…ExpressionBacterialPromoterpTacAvailable SinceJan. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
DJ-1 TEV site pET15b
Plasmid#60687PurposeRecombinant protein expression of Park7DepositorInsertDJ-1 (PARK7 Human)
Tags6xHisExpressionBacterialMutationTEV site was inserted (see comments below).PromoterT7Available SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
FB027
Plasmid#119712PurposeMultipartite assembly obtained by combining FB parts FB026+FB003 into pDGB3omega1. Used for fluorescent tagging of fungi (Hyg resistance +YFP) according to FungalBraid modular DNA assembly for ATMTDepositorInsertPgpdA::YFP::TtrpC(←)-PtrpC::hph::Ttub (→)
UseSynthetic BiologyAvailable SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-G-Flamp1-mut
Plasmid#188568Purposecontrol probe of G-Flamp1, insensitive to cAMPDepositorInsertG-Flamp1-mut
TagsHis tagExpressionMammalianPromoterCAGAvailable SinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo-CTRLg1
Plasmid#139450PurposeLentiviral vector with non-targeting gRNA and neomycin selectable markerDepositorInsertNon-targeting sgRNA inserted; resistance gene: neoR
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40
Plasmid#98928PurposeCre dependent AAV expression of Ruby2 spaghetti monster FLAG constructDepositorHas ServiceAAV1 and AAV5InsertRuby2-smFP-Flag
UseAAVExpressionMammalianPromoterCAGAvailable SinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Tet1 CD
Plasmid#70129PurposeExpress flag-tagged mouse Tet1 catalytic domain.DepositorInsertTen-Eleven Translocation 1 (Tet1 Mouse)
TagsflagExpressionMammalianMutationThe plasmid expresses TET1 catalytic domain.Available SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-H-Ras_wt
Plasmid#39503DepositorAvailable SinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IP spGFP-ERGIC53
Plasmid#38270DepositorInsertERGIC-53 (LMAN1 Human)
UseRetroviralTagsEGFP with Calreticulin signal peptide at N-termin…ExpressionMammalianMutationdeleted signal peptide (1-30 amino acids)Available SinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i3 ONE-GO
Plasmid#189737PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
TRE-mClover3
Plasmid#214922Purposeexpression vector control - contitutive expression of mClover3 aloneDepositorInsertmClover3
UseLentiviralAvailable SinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV-LMP2A-IRES-mCherry
Plasmid#220353PurposeExpresses EBV latent gene LMP2A in mammalian cellsDepositorAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB555
Plasmid#74885PurposeGateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-BaLgp120-8his
Plasmid#100925PurposeMammalian expression plasmid for gp120 subunit from the BaL HIV-1 strainDepositorInsertHIV-1 (BaL) Env
TagsCD5 leader sequence and Purification tag after K5…ExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable SinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMEH31 Lamp2b HA
Plasmid#168208PurposeMammalian expression of lysosome-associated membrane protein 2b fused to a C-terminal HA tagDepositorAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTL5
Plasmid#127967PurposeDHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locusDepositorInsertAAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
UseTALENExpressionMammalianPromoterCAGAvailable SinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PKCdelta-EGFP
Plasmid#178856PurposeMammalian expression of PKCδ fused to EGFPDepositorAvailable SinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Puro_siKO
Plasmid#86696PurposeTetracycline inducible expression of guide RNA targeted to AAVS1 locusDepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterH1 TOAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGMC00028 (aka pTS0057)
Plasmid#200832PurposeAAVS1 guide RNA cloned into the lentiSAM puro backboneDepositorInsertAAVS1 guide RNA
UseLentiviralExpressionMammalianAvailable SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI
Plasmid#115267PurposeExpresses LbuCas13a from a SUMO-tagged bacterial expression constructDepositorInsertLbuCas13a
TagsSUMOExpressionBacterialAvailable SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_AIO-GZV-SynZiFTR_Down-Tandem donor
Plasmid#229787PurposeBxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression (empty)DepositorInsertLacZ; ZF10-NS3-p65
UseSynthetic BiologyExpressionMammalianPromoterCAGAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRK5 beta4
Plasmid#16037DepositorInsertbeta4 (ITGB4 Human)
ExpressionMammalianAvailable SinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+DPP6
Plasmid#117272PurposeEncodes human DPP6 variant 1DepositorAvailable SinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-H-Ras_V12
Plasmid#39504DepositorAvailable SinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCP60 RS-GFP
Plasmid#122173Purpose"Binary vector for Agrobacterium tumefacience-mediated transformation of plant cells resulting in constitutive expression of cytoplasmatically localized RS-GFP"DepositorInsertRS-GFP
UseBinary vector for escherichia coli and agrobacter…ExpressionPlantPromoterCaMV 35SAvailable SinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
hE-cadherin/Δβ-catenin-pcDNA3
Plasmid#45772DepositorInserthE-cadherin/Δβ-catenin (CDH1 Human)
ExpressionMammalianMutationEnds at amino acid 844. Last 35 amino acids dele…PromoterCMVAvailable SinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only