Addgene

We narrowed to 28,326 results for: REP

Showing: 4681 - 4710 of 28326 results

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AAT-PB-CG2APtk

Plasmid

#86003

Purpose

homology donor for AAT locus with piggyBac-flanked eGFP_T2A_puroTK cassette

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

eGFP_T2A_puro_dTK

Use

Piggybac exciseable

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pIE-hα2M-H6

Plasmid

#128492

Purpose

Expresses human α2M (from S24 to N1473) in insect cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

A2M (A2M Human)

Use

Tags

His tag (6x)

Expression

Insect

Mutation

Promoter

IE1

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMTB2-Avi-Cerulean-RanGAP

Plasmid

#79878

Purpose

Beta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)

Use

Tags

Avi

Expression

Bacterial

Mutation

Promoter

Beta-actin2

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pTYF-PRSx8-AstR-p2A-GFP

Plasmid

#159632

Purpose

Lentiviral expression of the Allatostatin receptor, P2A GFP under the PRSx8 promoter

Depositor

Andrew Allen

Article

Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

Insert

AstR (AstA-R1 Fly)

Use

Lentiviral

Tags

GFP and P2A

Expression

Mammalian

Mutation

Promoter

PRSx8

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-FullLength-TET1-LLAA(20)

Plasmid

#124397

Purpose

Full-length mutant TET1 (L897A/L900A) used to PiggyBAC rescue the function of TET1/2 DKO cells

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

FLAG-FullLength-TET1 with L897A/L900A mutations (TET1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

L897A/L900A mutations (full length protein is 213…

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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GFP scFv [N86/44]

Plasmid

#206743

Purpose

Mammalian Expression of GFP scFV. Derived from hybridoma N86/44 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GFP (Aequorea victoria) recombinant scFV

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO-3XFLAG Halo K164A/H165A nsp1

Plasmid

#175421

Purpose

Expresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 nsp1

Use

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMTB2-NLS-BirA-2A-mCherry_Ras

Plasmid

#80067

Purpose

Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Mutation

Promoter

Beta-actin2

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMTB2-cytoBirA-2A-mCherry_Ras

Plasmid

#80066

Purpose

Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Mutation

Promoter

Beta-actin2

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-Phobos

Plasmid

#98166

Purpose

blue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.

Depositor

Peter Hegemann

Article

Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

Insert

Synthetic construct Phobos gene

Use

Tags

mCherry

Expression

Mammalian

Mutation

T59S, E83N, E90Q, E101S, V117R, E123S, T159G, G16…

Promoter

CMV (+enhancer)

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CIP2A(1-905) R522D V5 His

Plasmid

#119289

Purpose

CIP2A R522D V5 overexpression, CIP2A dimer defective mutant

Depositor

Jukka Westermarck

Article

Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

Insert

KIAA1524 (CIP2A Human)

Use

Tags

V5 His

Expression

Mammalian

Mutation

R522D

Promoter

T7

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-IgKL-mNG

Plasmid

#183465

Purpose

To produce AAV with the P3 promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

IgKL-mNeonGreen

Use

AAV

Tags

IgKL secretory signal

Expression

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pJS699

Plasmid

#168779

Purpose

Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Insert

Spike RBD (S betacoronavirus)

Use

Tags

Cmyc

Expression

Mutation

N343Q

Promoter

GAL1

Available since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shETV1-A

Plasmid

#74973

Purpose

shETV1 shRNA

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ETV1 (ETV1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

Plasmid

#124660

Purpose

reporter protein which emits bright blue fluorescence

Depositor

Yutaka Kuroda

Article

Wu et al Sci Rep. 2020 Nov 18;10(1):20069. doi: 1

Insert

Gaussia luciferase-C9D-TGinsert

Use

Tags

IEGRTMGDDDGDDDGDDD（FactorXa,TMG,C9D-Tag） and MHHH…

Expression

Bacterial

Mutation

changed glutamic acid 100 to alanine, and glycine…

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-Aurora

Plasmid

#98167

Purpose

red-shifted artificial anion conducting channelrhodopsin (aACR). Activation up to 600 nm; off-kinetics 260 ms. Codon optimized for mammalian expression.

Depositor

Peter Hegemann

Article

Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

Insert

Synthetic construct Aurora gene

Use

Tags

mCherry

Expression

Mammalian

Mutation

V59S, E83N, E90Q, E101S, V117R, E123S, P242R, A24…

Promoter

CMV (+enhancer)

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pKI-cjFOXP2-hum

Plasmid

#129757

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

FOXP2 (LOC100411307 C. jacchus (common marmost))

Use

Marmoset targeting

Tags

Expression

Mutation

T301N/N323S

Promoter

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pMT-ubb-Avi-Cerulean-Rpl10a

Plasmid

#79882

Purpose

Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)

Use

Tags

Avi

Expression

Bacterial

Mutation

Promoter

Ubiquitin

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pNHT7::RPL15::hGluc

Plasmid

#186768

Purpose

An mRNA production vector encoding human codon-optimized gaussia luciferase (hGluc) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Gaussia luciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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sgRNA BRCA1 C64Y

Plasmid

#139326

Purpose

Plasmid expressing a sgRNA to introduce BRCA1 C64Y using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA1 C64Y using base editing

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA BRCA1 E638K

Plasmid

#139327

Purpose

Plasmid expressing a sgRNA to introduce BRCA1 E638K using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA1 E638K using base editing

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pcmv3-IRG1-H159Q-HA

Plasmid

#198183

Purpose

Mammalian expression of HA-tagged human ACOD1 (IRG1) H159Q

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

ACOD1 (ACOD1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

The histidine in IRG1 at position 159 mutates to …

Promoter

CMV

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_mpx_AsCas12a(dual-gRNA)_h7SK(PacI-ClaI)_U6(I-SceI-NheI)_PGK-puro

Plasmid

#189635

Purpose

Lentiviral expression of double dual-AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

h7SK arrayed Cas12a gRNA cassette, hU6 arrayed Cas12a gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGMC00034 (aka pMC0235)

Plasmid

#210751

Purpose

Non-targeting guide RNA

Depositor

Raj Chari

Article

Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

Insert

Non-targeting guide RNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pelB-MBP-BB0323-ss

Plasmid

#137067

Purpose

E. coli expression clone (T7lac promoter) for mature BB0323 (aa 20-377) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAC66700.1

Use

Tags

PelB signal peptide + His10 + maltose binding pro…

Expression

Bacterial

Mutation

mature BB0323: lacks the BB0323 signal peptide

Promoter

T7lac

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSN25

Plasmid

#100110

Purpose

expresses a chimeric SOX2-repressor fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCitrine-P2A-SOX2 fused to full length HP1 alpha (SOX2, CBX5 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSicoR-sh4EBP2

Plasmid

#81121

Purpose

expresses an shRNA targeting murine 4E-BP2

Depositor

Angelique Bordey

Article

Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

Insert

4E-BP2 shRNA (Eif4ebp2 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO R99A CoV-2 nsp1 3xFLAG

Plasmid

#176061

Purpose

Expresses R99A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV-2 nsp1 (ORF1ab SARS-CoV-2)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2 P1-6A

Plasmid

#51251

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848,923,956 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 789,805,817,848,923,956 mutated to Ala

Promoter

EF1a

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pLKO.1 shETV1-B

Plasmid

#74974

Purpose

shETV1 shRNA

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ETV1 (ETV1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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