4,168 results

1. AAVS1-TALEN-L

   Plasmid

   #59025

   Purpose

   TALEN designed to target the human AAVS1/PPP1R12C locus

   Depositor

   Danwei Huangfu

   Article

   Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

   Insert

   AAVS1-TALEN-L
   

   Use

   AAV and TALEN

   Tags

   3xFlag and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-TALEN-R

   Plasmid

   #59026

   Purpose

   TALEN designed to target the human AAVS1/PPP1R12C locus

   Depositor

   Danwei Huangfu

   Article

   Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

   Insert

   AAVS1-TALEN-R
   

   Use

   AAV and TALEN

   Tags

   3xFlag and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3.1(+)-CMV-bArrestin2-TEV

   Plasmid

   #107245

   Purpose

   Vector for preparation of stable cell lines expressing Barrestin2-TEV fusion protein in lieu of the HTLA cell line.

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   ARRB2
   

   Tags

   TEV(NIA), tobacco etch virus NIA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. L4440

   Plasmid

   #1654

   Depositor

   Andrew Fire

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   RNAi

   Tags

   T7p, T7p, lacZN, OriF1>>, OriF1<<

   Expression

   Worm

   Available Since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLJC5-Tmem192-3xHA

   Plasmid

   #102930

   Purpose

   Lentiviral expression of a lysosomal tag (Tmem192-HA)

   Depositor

   David Sabatini

   Article

   Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

   Insert

   Tmem192 (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Promoter

   ubc

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. VAMP2-pHmScarlet

   Plasmid

   #166890

   Purpose

   For vesicle exocytosis visualization using pHmScarlet

   Depositor

   Pingyong Xu

   Article

   Liu et al Nat Commun. 2021 Mar 3;12(1):1413. doi:

   Insert

   VAMP2 (Vamp2 Mouse)
   

   Tags

   pHmScarlet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV R-CEPIA1er

   Plasmid

   #58216

   Purpose

   Red fluorescent indicator for calcium signaling in the endoplasmic reticulum

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   R-CEPIA1er
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   R-GECO1 E300D M305L D329N F332I E336D N346K F361W…

   Promoter

   CMV

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCBASceI

   Plasmid

   #26477

   Purpose

   I-SceI endonuclease expression vector with mammalian promoter to introduce a DSB at a genomic I-SceI site

   Depositor

   Maria Jasin

   Article

   Richardson et al Genes Dev. 1998 Dec 15. 12(24):3831-42.

   Insert

   pCBASceI
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-15F11-HA-mEGFP

   Plasmid

   #129590

   Purpose

   The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

   Depositor

   Tim Stasevich

   Article

   Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

   Insert

   Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti-EF1a-dCas9-VPR-Puro

    Plasmid

    #99373

    Purpose

    3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    (Sp)dCas9-VPR-P2A-Puro
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-Ub

    Plasmid

    #11928

    Purpose

    Used for in vivo monitoring of ubiquitin localization.

    Depositor

    Nico Dantuma

    Article

    Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

    Insert

    Ubiquitin (UBC Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCasSA

    Plasmid

    #98211

    Purpose

    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

    Depositor

    Quanjiang Ji

    Article

    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX6P1-GFP-Nanobody

    Plasmid

    #61838

    Purpose

    Expresses GST-tagged anti-GFP Nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    GFP-Nanobody
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

    Plasmid

    #71237

    Purpose

    Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A GFP
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Mutation

    D10A and H840A

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pORTMAGE-Ec1

    Plasmid

    #138474

    Purpose

    Enables high-efficiency recombineering in E. coli

    Depositor

    George Church

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

    Inserts

    CspRecT
    mutL E32K
    

    Expression

    Bacterial

    Mutation

    E32K

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-2xFLAG-SREBP-1c

    Plasmid

    #26802

    Depositor

    Timothy Osborne

    Article

    Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

    Insert

    SREBP-1c (SREBF1 Human)
    

    Tags

    2XFLAG

    Expression

    Mammalian

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV/SB11

    Plasmid

    #26552

    Depositor

    Perry Hackett

    Article

    Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

    Insert

    Sleeping Beauty
    

    Expression

    Bacterial

    Mutation

    Methionine 243 to Glutamine Threonine 136 to Arg…

    Available Since

    Oct. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. phH1-gRNA

    Plasmid

    #53186

    Purpose

    Expresses the S. pyogenes sgRNA from the H1 promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human H1

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE_puro

    Plasmid

    #118692

    Purpose

    Empty vector to express overexpression cDNA

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Type

    Empty backbone

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pmU6-gRNA

    Plasmid

    #53187

    Purpose

    Expresses the S. pyogenes sgRNA from the mouse U6 promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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