4,200 results

1. pET21a-Streptavidin-Alive

   Plasmid

   #20860

   Purpose

   Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

   Depositor

   Alice Ting

   Article

   Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

   Insert

   Streptavidin-Alive
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Wt gene

   Available Since

   April 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTP1122

   Plasmid

   #104159

   Purpose

   Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
   

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. eIF2a 3

   Plasmid

   #21809

   Depositor

   David Ron

   Insert

   eIF2a (EIF2S1 Human)
   

   Expression

   Mammalian

   Mutation

   S52D

   Available Since

   Sept. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-UbC-rtTA-Ngn2:2A:Ascl1

   Plasmid

   #127289

   Purpose

   all-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)

   Depositor

   Fred Gage

   Article

   Herdy et al Elife. 2019 May 17;8. pii: 41356. doi: 1

   Inserts

   Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
   Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
   

   Use

   Lentiviral

   Tags

   Ngn2 and Ascl1 linked via 2A

   Expression

   Mammalian

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-mCherry

   Plasmid

   #128744

   Purpose

   Expresses mCherry in mammalian cells

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-HA-OTUB1

   Plasmid

   #22551

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   OTUB1 (OTUB1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGFPGUSPlus

   Plasmid

   #64401

   Purpose

   binary plant vector expressing GFP and GUS, both driven by a 35S promoter

   Depositor

   Claudia Vickers

   Article

   Vickers et al Biotechnol Lett. 2007 Nov;29(11):1793-6.

   Inserts

   GFP
   GUSPlus
   

   Use

   Binary vector for plant transformation

   Tags

   His

   Expression

   Plant

   Mutation

   S65T

   Promoter

   35S

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP_mNG2(11)_Clathrin light chain

   Plasmid

   #82608

   Purpose

   Expresses mNG2(11) tagged clathrin light chain in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   mNG2(11)_Clathrin light chain
   

   Expression

   Mammalian

   Available Since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-dCas9-KRAB-blast

   Plasmid

   #89567

   Purpose

   Plasmid expression dCas9 protein in fusion with KRAB domain

   Depositor

   Gary Hon

   Article

   Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   Blasticidin resistance gene and KRAB

   Mutation

   D10A and H840A

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO5.sgRNA.EFS.GFP

    Plasmid

    #57822

    Purpose

    3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    eGFP
    

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAX APP Swe/Ind

    Plasmid

    #30145

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    human APP 695 Swedish/Indiana mutation (APP Human)
    

    Expression

    Mammalian

    Mutation

    Swedish and Indiana; K595N, M596L and V642F

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21a-BirA

    Plasmid

    #20857

    Depositor

    Alice Ting

    Article

    Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 1

    Insert

    BirA
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRIP-CMV-GFP-FLAG-cGAS

    Plasmid

    #86675

    Purpose

    Expresses catalytically-active GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

    Depositor

    Nicolas Manel

    Article

    Raab et al Science. 2016 Apr 15;352(6283):359-62. d

    Insert

    GFP-FLAG-cGAS
    

    Use

    Lentiviral

    Available Since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiCRISPRv2 puro

    Plasmid

    #98290

    Purpose

    Variant of lentiCRISPRv2 that confers puromycin resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Promoter

    EF-1a

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. mNeonGreen-mTurquoise2

    Plasmid

    #98886

    Purpose

    Produces a fusion between mTurquoise2 and mNeonGreen. It can be used as a positive control for FRET

    Depositor

    Dorus Gadella

    Article

    Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

    Insert

    mNeonGreen-mTurquoise2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-p300

    Plasmid

    #23252

    Depositor

    Warner Greene

    Article

    Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

    Insert

    p300 (EP300 Human)
    

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-TetO-hNGN2-eGFP-Puro

    Plasmid

    #79823

    Purpose

    Expresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.

    Depositor

    Kristen Brennand

    Article

    Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

    Insert

    hNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)
    

    Use

    Lentiviral

    Promoter

    TetO

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. BPK1520

    Plasmid

    #65777

    Purpose

    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SpCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry-CD9-10

    Plasmid

    #55013

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    CD9 (CD9 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 EYFP H148Q/I152L

    Plasmid

    #25872

    Depositor

    Peter Haggie

    Article

    Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

    Insert

    EYFP H148Q/I152L
    

    Expression

    Mammalian

    Mutation

    EYFP from pEYFP (Clontech) with H148Q and I152L m…

    Available Since

    Aug. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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