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AAV-GFP/Cre

Plasmid

#49056

Purpose

Adeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.

Depositor

Fred Gage

Publication

Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

Insert

Cre

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

EGFP, Myc, and NLS

Expression

Mammalian

Promoter

CMV

Available since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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pCAG-Cre

Plasmid

#13775

Purpose

Mammalian expression of Cre recombinase from the broadly active CAG promoter/enhancer

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Cre

Use

Cre/Lox

Tags

Myc

Expression

Mammalian

Promoter

CAG

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

126 Articles

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pSpCas9n(BB) (PX460)

Plasmid

#48873

Purpose

Cas9n (D10A nickase mutant) from S. pyogenes

Depositor

Feng Zhang

Publication

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Has service

Cloning Grade DNA

Insert

hSpCas9n

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Promoter

Cbh

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pU6-Sp-pegRNA-HEK3_CTT_ins

Plasmid

#132778

Purpose

S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

Depositor

David Liu

Publication

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

HEK3_CTT_ins pegRNA

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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MS2-P65-HSF1_GFP

Plasmid

#61423

Purpose

Expresses the MS2-P65-HSF1 activator helper complex with a 2A GFP. 3rd generation lentiviral vector.

Depositor

Feng Zhang

Publication

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Insert

MS2(N55K)-P65-HSF1_2A_GFP (HSF1 Human, Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1A

Available since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

49 Articles

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pBabe-Puro-BRAF-V600E

Plasmid

#15269

Purpose

Retroviral expression of BRAF V600E (the V600E mutation has been found in cancer patients, confers increased kinase activity, and is transforming)

Depositor

William Hahn

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

BRAF (BRAF Human)

Use

Retroviral

Tags

His and Myc

Expression

Mammalian

Mutation

Valine 600 to Glutamate (mutation has been found …

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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pC016 - LwCas13a guide expression backbone with U6 promoter

Plasmid

#91906

Purpose

Backbone for cloning LwCas13a guides under U6 promoter

Depositor

Feng Zhang

Publication

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Has service

Cloning Grade DNA

Insert

LwCas13a Guide site

Expression

Mammalian

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pGP-CMV-NES-jRGECO1a

Plasmid

#61563

Purpose

Red fluorescent calcium sensor protein

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

NES-jRGECO1a

Tags

nuclear export signal

Expression

Mammalian

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

35 Articles

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pDEST-HisMBP

Plasmid

#11085

Depositor

David Waugh

Publication

Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

Has service

Cloning Grade DNA

Type

Empty backbone

Tags

6xHis and MBP

Expression

Bacterial

Promoter

Tac (lactose/IPTG inducible)

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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HDAC6 Flag

Plasmid

#13823

Purpose

Mammalian expression of human histone deacetylase 6 with flag tag

Depositor

Eric Verdin

Publication

Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

Insert

HDAC6 (HDAC6 Human)

Tags

flag

Expression

Mammalian

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

35 Articles

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pTet-O-Ngn2-puro

Plasmid

#52047

Purpose

3rd generation lentiviral vector, expresses Ngn2 and puromycin resistent gene under control of TetON promoter

Depositor

Marius Wernig

Publication

Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

Insert

Ngn2 (Neurog2 Mouse)

Use

Lentiviral

Tags

T2A-and puromycin resistant cassette

Expression

Mammalian

Promoter

tet-on

Available since

April 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

88 Articles

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pSin-EF2-Nanog-Pur

Plasmid

#16578

Depositor

James Thomson

Publication

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens Nanog homeobox (NANOG Human)

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

41 Articles

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pSin-EF2-Oct4-Pur

Plasmid

#16579

Depositor

James Thomson

Publication

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1) (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

37 Articles

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3xAP1pGL3 (3xAP-1 in pGL3-basic)

Plasmid

#40342

Depositor

Alexander Dent

Publication

Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

Insert

3xAP-1

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Contains three canonical AP-1 binding sites (TGAC…

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

57 Articles

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pTwist-SARS-CoV-2 Δ18 B.1.1.529

Plasmid

#179907

Purpose

Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Publication

Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014

Insert

SARS-CoV-2 B.1.1.529 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

A67V; Δ69-70; T95I; G142D; Δ143-145; N211I; Δ212;…

Promoter

CAG

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pXR004: CasRx pre-gRNA cloning backbone

Plasmid

#109054

Purpose

hU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs

Depositor

Patrick Hsu

Publication

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Has service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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OKSIM

Plasmid

#24603

Depositor

Jose Cibelli

Publication

Ross et al Stem Cells Dev. 2010 Aug;19(8):1221-9

Insert

Oct4 KLF4 Sox2 c-Myc (SOX2, POU5F1, MYC, KLF4 Human)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

Oct4-2A-KLF4-2A-Sox2-IRES-Myc

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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800 pSG5L HA PTEN wt

Plasmid

#10750

Depositor

William Sellers

Publication

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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mEGFP-ER-5a

Plasmid

#56455

Purpose

Localization: Endoplasmic Reticulum, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Insert

ER 5a (CALR Human)

Tags

mEGFP

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available since

Dec. 23, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pSLCAR-CD19-BBz

Plasmid

#135992

Purpose

Modular 41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Scott McComb

Publication

Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

Insert

FMC63-41BB-3z-P2A-EGFP (CD247, CD28, TNFRSF9 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a-short

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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