Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 14,451 results for: Cas9

Showing: 301 - 320 of 14451 results

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pLenti_Cas9_GFP_AAVS1_sg2

Plasmid

#254530

Purpose

AAVS1 knockout, control guide

Depositor

Jason Sheltzer

Article

Klaske M. et al bioRxiv 2026.04.26.720636

Insert

AAVS1

Use

CRISPR and Lentiviral

Tags

FLAG-SV40NLS and nucleoplasminNLS-P2A-GFP

Available Since

May 11, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_Cas9_GFP_AAVS1_sg3

Plasmid

#254531

Purpose

AAVS1 knockout, control guide

Depositor

Jason Sheltzer

Article

Klaske M. et al bioRxiv 2026.04.26.720636

Insert

AAVS1

Use

CRISPR and Lentiviral

Tags

FLAG-SV40NLS and nucleoplasminNLS-P2A-GFP

Available Since

May 11, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_Cas9_GFP_ROSA26_sg1

Plasmid

#254532

Purpose

ROSA26 control guide

Depositor

Jason Sheltzer

Article

Klaske M. et al bioRxiv 2026.04.26.720636

Insert

ROSA26

Use

CRISPR and Lentiviral

Tags

FLAG-SV40NLS and nucleoplasminNLS-P2A-GFP

Available Since

May 11, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_Cas9_GFP_CDC7_sg1

Plasmid

#254533

Purpose

CDC7 knockout

Depositor

Jason Sheltzer

Article

Klaske M. et al bioRxiv 2026.04.26.720636

Insert

CDC7 (CDC7 Human)

Use

CRISPR and Lentiviral

Tags

FLAG-SV40NLS and nucleoplasminNLS-P2A-GFP

Available Since

May 11, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_Cas9_GFP_CDC7_sg2

Plasmid

#254534

Purpose

CDC7 knockout

Depositor

Jason Sheltzer

Article

Klaske M. et al bioRxiv 2026.04.26.720636

Insert

CDC7 (CDC7 Human)

Use

CRISPR and Lentiviral

Tags

FLAG-SV40NLS and nucleoplasminNLS-P2A-GFP

Available Since

May 11, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_Cas9_GFP_CDC7_sg3

Plasmid

#254535

Purpose

CDC7 knockout

Depositor

Jason Sheltzer

Article

Klaske M. et al bioRxiv 2026.04.26.720636

Insert

CDC7 (CDC7 Human)

Use

CRISPR and Lentiviral

Tags

FLAG-SV40NLS and nucleoplasminNLS-P2A-GFP

Available Since

May 11, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_Cas9_GFP_CDC7_sg4

Plasmid

#254536

Purpose

CDC7 knockout

Depositor

Jason Sheltzer

Article

Klaske M. et al bioRxiv 2026.04.26.720636

Insert

CDC7 (CDC7 Human)

Use

CRISPR and Lentiviral

Tags

FLAG-SV40NLS and nucleoplasminNLS-P2A-GFP

Available Since

May 11, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_Cas9_GFP_CDK9_sg1

Plasmid

#254537

Purpose

CDK9 knockout

Depositor

Jason Sheltzer

Article

Klaske M. et al bioRxiv 2026.04.26.720636

Insert

CDK9 (CDK9 Human)

Use

CRISPR and Lentiviral

Tags

FLAG-SV40NLS and nucleoplasminNLS-P2A-GFP

Available Since

May 11, 2026

Availability

Academic Institutions and Nonprofits only

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pCas9_antiBFP

Plasmid

#220922

Purpose

Expresses Cas9 with a BFP targeting crRNA for use in targeting the BFP plasmid in a cell based assay

Depositor

Ryan Jackson

Article

Walters et al J Microbiol Biol Educ. 2026 Apr 30;27(1)

Insert

pCas9

Expression

Bacterial

Available Since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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pCas9_antiGFP

Plasmid

#220923

Purpose

Expresses Cas9 with a GFP targeting crRNA for use in targeting the GFP plasmid in a cell based assay

Depositor

Ryan Jackson

Article

Walters et al J Microbiol Biol Educ. 2026 Apr 30;27(1)

Insert

pCas9

Expression

Bacterial

Available Since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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pCas9_antiOFP

Plasmid

#220924

Purpose

Expresses Cas9 with a OFP targeting crRNA for use in targeting the OFP plasmid in a cell based assay

Depositor

Ryan Jackson

Article

Walters et al J Microbiol Biol Educ. 2026 Apr 30;27(1)

Insert

pCas9

Expression

Bacterial

Available Since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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pGCas9_scr

Plasmid

#207567

Purpose

GeoCas9-mediated cleavage of genomic DNA in E. coli

Depositor

John van der Oost

Article

Trasanidou et al Commun Biol. 2023 Oct 13;6(1):1042. doi:

Insert

Plac/tet_GeoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)

Expression

Bacterial

Mutation

Wild-type

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pdTCas9_scr

Plasmid

#207568

Purpose

dThermoCas9-mediated silencing of genomic gene in E. coli

Depositor

John van der Oost

Article

Trasanidou et al Commun Biol. 2023 Oct 13;6(1):1042. doi:

Insert

Plac/tet_dThermoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)

Expression

Bacterial

Mutation

ThermoCas9(D8A,H582A)

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pdGCas9_scr

Plasmid

#207569

Purpose

dGeoCas9-mediated silencing of genomic gene in E. coli

Depositor

John van der Oost

Article

Trasanidou et al Commun Biol. 2023 Oct 13;6(1):1042. doi:

Insert

Plac/tet_dGeoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)

Expression

Bacterial

Mutation

GeoCas9(D8A,H582A)

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAcrTCas9_scr

Plasmid

#207571

Purpose

AcrIIC1-mediated inhibition of ThermoCas9 cleavage activity in E. coli

Depositor

John van der Oost

Article

Trasanidou et al Commun Biol. 2023 Oct 13;6(1):1042. doi:

Insert

Prha_AcrIIC1Nme_Plac/tet_ThermoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)

Expression

Bacterial

Mutation

Wild-type

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAcrGCas9_scr

Plasmid

#207572

Purpose

AcrIIC1-mediated inhibition of GeoCas9 cleavage activity in E. coli

Depositor

John van der Oost

Article

Trasanidou et al Commun Biol. 2023 Oct 13;6(1):1042. doi:

Insert

Prha_AcrIIC1Nme_Plac/tet_GeoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)

Expression

Bacterial

Mutation

Wild-type

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA3717

Plasmid

#240491

Purpose

CRISPRi-mediated Knockdown of RPA3717

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA3701

Plasmid

#240490

Purpose

CRISPRi-mediated Knockdown of RPA3701

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSSBIO32_dCas9_RPA1580

Plasmid

#240488

Purpose

CRISPRi-mediated Knockdown of RPA1580

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW124_dCas9

Plasmid

#244821

Purpose

Mammalian expression of catalytically inactive Cas9 (dCas9)

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

dCas9 (WT)

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Mutation

D10A/H840A

Promoter

CAG, U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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Showing: 301 - 320 of 14451 results

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