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We narrowed to 10,071 results for: EPO

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Showing: 8051 - 8100 of 10071 results

  6. p420-3

    Plasmid
    #71257
    Purpose
    luciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in front of hGM-CSF -55 to +28 minimal promoter
    Depositor
    Peter Cockerill
    Article
    Johnson et al Mol Cell Biol. 2004 Sep;24(18):7914-30.
    Insert
    3 copies of the GM420 NFAT-AP-1 site (CSF2 Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Promoter
    GM-CSF minimal
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    71257-plasmid-map-sequence-id-131889

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  7. pCAG-memRFP-3xPax7gRNA

    Plasmid
    #224569
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    mRFP1
    Use
    CRISPR
    Tags
    Membrane localization signal
    Mutation
    Codon optimized
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224569-plasmid-map-sequence-id-451497

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  12. pDual-eGFP(Y203)

    Plasmid
    #63559
    Purpose
    Expression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(Y203)
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Changed threonine at position 203 with respect to…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. AAV-GfaABC1D-lck-smFLAG-4x6T-WPRE

    Plasmid
    #196414
    Purpose
    Astrocytic expression of membrane-targeted FLAG spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette
    Depositor
    Stanley Thomas Carmichael
    Article
    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:
    Insert
    Lck-smFLAG
    Use
    AAV; Astrocyte-selective
    Tags
    Lck
    Expression
    Mammalian
    Promoter
    CAG and GfaABC1D
    Available Since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    196414-plasmid-map-sequence-id-385111

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  15. pFRLuc-CoV2-delStem1

    Plasmid
    #177616
    Purpose
    Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. 3-nucleotide deletion in Stem 1 (negative control).
    Depositor
    Junjie Guo
    Article
    Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11
    Insert
    SARS-CoV-2 FSE
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    3-nucleotide (UAC) deletion in Stem 1
    Available Since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177616-plasmid-map-sequence-id-350042

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  19. Fireworks RFP[PTC+] (AVA2626)

    Plasmid
    #85444
    Purpose
    Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.
    Depositor
    Joan Steitz
    Article
    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:
    Insert
    tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR
    Use
    Minimal backbone fragment for low-copy bacterial …
    Expression
    Mammalian
    Available Since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85444-plasmid-map-sequence-id-171527

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  23. LSB-hsa-miR-9-5p

    Plasmid
    #103749
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-9-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-9-5p target
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103749-plasmid-map-sequence-id-196546

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  30. pFRLuc-CoV2-delSS

    Plasmid
    #177615
    Purpose
    Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. Slippery sequence (SS) deleted (negative control).
    Depositor
    Junjie Guo
    Article
    Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11
    Insert
    SARS-CoV-2 FSE
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Slippery sequence deleted
    Available Since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177615-plasmid-map-sequence-id-350040

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  33. pAAV-anti-TNFalpha(Ozoralizumab) PAGER(TF)

    Plasmid
    #230001
    Purpose
    Expresses anti-TNFalpha PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
    Depositor
    Alice Ting
    Article
    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-
    Insert
    IL2SP-Aro6-Ozoralizumab-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    Use
    AAV
    Expression
    Mammalian
    Mutation
    V360A/R361A on KORD
    Promoter
    CMV
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230001-plasmid-map-sequence-id-457205

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  34. pAAV-anti-CCL2(8E10) PAGER(TF)

    Plasmid
    #230002
    Purpose
    Expresses anti-CCL2 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
    Depositor
    Alice Ting
    Article
    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-
    Insert
    IL2SP-Aro6-8E10-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    Use
    AAV
    Expression
    Mammalian
    Mutation
    V360A/R361A on KORD
    Promoter
    CMV
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230002-plasmid-map-sequence-id-457201

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  35. pAAV-anti-mCherry(LaM6) PAGER(TF)

    Plasmid
    #229998
    Purpose
    Expresses anti-mCherry PAGER(TF) (high sensitivity) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
    Depositor
    Alice Ting
    Article
    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-
    Insert
    IL2SP-Aro6-LaM6-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    Use
    AAV
    Expression
    Mammalian
    Mutation
    V360A/R361A on KORD
    Promoter
    CMV
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    229998-plasmid-map-sequence-id-457195

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  37. Fireworks GFP[PTC+] (AVA2600)

    Plasmid
    #85446
    Purpose
    Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.
    Depositor
    Joan Steitz
    Article
    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:
    Insert
    tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
    Use
    Minimal backbone fragment for low-copy bacterial …
    Expression
    Mammalian
    Available Since
    Feb. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    85446-plasmid-map-sequence-id-171530

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  39. ROSA26(MultiFPsΔPuro)

    Plasmid
    #140759
    Purpose
    Targeting vector to Gt(ROSA)26 locus for conditional expression of distinct FPs (Venus, mCherry, and mCerulean) responding to each site-specific-recombinase activity (Cre, Dre, and phiC31o).
    Depositor
    Hideyuki Okano
    Article
    Serizawa et al Development. 2019 Nov 4;146(21). pii: de
    Inserts
    puromycin-N-acetyltransferase
    Venus
    mCherry
    mCerulean
    Use
    Mouse Targeting
    Promoter
    CAGGS
    Available Since
    June 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140759-plasmid-map-sequence-id-311014

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  40. aCat DeltaTS (delta570 to 670 aa in aCat TS WT)

    Plasmid
    #101300
    Purpose
    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin. See Depositor Comments for plasmid construction.
    Depositor
    Alpha Yap
    Article
    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.
    Insert
    alpha-Catenin
    Expression
    Mammalian
    Mutation
    Contains amino acids 1-509, 670-698, and 699-906 …
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    101300-plasmid-map-sequence-id-246103

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  41. LSB-hsa-miR-199a-3p

    Plasmid
    #103319
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-199a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-199a-3p target
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103319-plasmid-map-sequence-id-195687

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  42. pEND-229_Cacnes_EforCP

    Plasmid
    #225615
    Purpose
    Replicative vector for red fluorescent reporter protein eForCP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+eForCP
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    EforCP
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225615-plasmid-map-sequence-id-451383

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  43. pEND-226_Cacnes_mCitrine

    Plasmid
    #225613
    Purpose
    Replicative vector for green fluorescent reporter protein mCitrine strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCitrine
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    mCitrine
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225613-plasmid-map-sequence-id-451363

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  44. p2R3a-3AT

    Plasmid
    #71273
    Purpose
    Entry clone containing 3AT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Ari Pekka Mähönen
    Article
    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246
    Insert
    T3A ribulose-1,5-bisphosphate carboxylase 3A subunit terminator
    Use
    Gateway
    Available Since
    Dec. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    71273-plasmid-map-sequence-id-129830

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  49. PB-CAG-DDdCas9VPP300-T2A-GFP-IRES-Neo

    Plasmid
    #102887
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-P300-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102887-plasmid-map-sequence-id-262467

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  50. VanGlow-RR-DSCP

    Plasmid
    #83339
    Purpose
    for Gateway cloning of enhancer elements upstream of the DSCP and mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.
    Depositor
    Cheng-Yu Lee
    Article
    Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    mCherry::Renilla(nls)
    Expression
    Insect
    Promoter
    Drosophila Synthetic Core Promoter
    Available Since
    Dec. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    83339-plasmid-map-sequence-id-158654

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Showing: 8051 - 8100 of 10071 results