We narrowed to 18,642 results for: RAN-1
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Plasmid#174667PurposeExpresses GFP-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.DepositorInsertGFP-endoNA2 fusion protein
UseTagsHistagExpressionBacterialMutationPromoterT5Available SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-dSaCas9-KRAB-bGHpA
Plasmid#106219PurposeAAV expressing deactivated S. aureus Cas9 fused to a KRAB repressorDepositorInsertdead S. aureus Cas9 KRAB
UseAAV and CRISPRTagsHA-NLSExpressionMammalianMutationD10A and N580APromoterCMVAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-attB-PUb-GFP
Plasmid#183911PurposeattB-site containing docking plasmid for genomic insertion into attP site, encoding GFP under control of the Aedes aegypti PUb promoterDepositorInsertGFP
UseTagsExpressionMutationPromoterAedes aegypti PUbAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUIN
Plasmid#184267PurposeA binary plasmid derived from pCambia1300, with an insert that is a full-length cDNA of CPSMV RNA1 flanked by CaMV 35S promoter and terminator.DepositorInsertCPSMV RNA1 cDNA with 4 introns
UseTagsExpressionPlantMutationS1098P, K1651RPromoterCaMV 35S with duplicated enhancerAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-FLAG-HA-mmuMettl3
Plasmid#207122PurposeLentiviral plasmid to express mouse Mettl3DepositorAvailable SinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-wt
Plasmid#60728PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.DepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-UBC-hAAT-IRES-ZsGreen-W
Plasmid#126702Purposelentiviral bicistronic exprression of both human alpha-1 anitrypsin and ZsGreenDepositorInsertAlpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen" (SERPINA1 Human)
UseLentiviralTagsExpressionMutationPromoterUBCAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMMP-BiP-IRES-mRFP
Plasmid#36975DepositorAvailable SinceMay 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.0-Pct5.1-crRNA(AarI)
Plasmid#191633PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer), entry plasmid for crRNA cloningDepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-T7-hOsa2
Plasmid#17987DepositorAvailable SinceAug. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dSaCas9-KRAB-T2A-PuroR
Plasmid#106249PurposeLentiviral vector with puro selection for expression of S. aureus dCas9-KRABDepositorInsertdead S. aureus Cas9 KRAB T2A PuroR
UseCRISPR and LentiviralTagsHA-NLS and NLS-KRABExpressionMammalianMutationD10A and N580APromoterhUbCAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag Erg
Plasmid#66977PurposeExpression plasmid for mouse Erg; adds Flag tag to the N-terminus of proteinDepositorAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS
Plasmid#107320PurposeExpresses SpCas9 fused to SaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 is fused to SaCas9PromoterCMV IE94Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNLS-2xBFP(4C)
Plasmid#176151PurposeEncodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.DepositorInsertNLS-2xBFP(4C)
UseTags6xHis tag and SV40 nuclear localization sequence …ExpressionBacterialMutationCompared with EGFP, the two BFP domains have the …PromoterPtrcAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-EGFP-SOS1
Plasmid#178860PurposeMammalian expression of SOS1 fused to EGFPDepositorAvailable SinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKSB-AeU6-3gRNA
Plasmid#183914PurposegRNA-expressing plasmid under Aedres aegypti AAEL017763 U6 promoterDepositorInsertgRNA scaffold for protospacer cloning into BbsI sites
UseTagsExpressionMutationPromoterAedes aegypti U6 promoter (AAEL017763)Available SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM9-BetaGal-8C
Plasmid#172489PurposePlasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequenceDepositorInsertM9-BetaGal-8C
UseTags6xHis and M9 nuclear localization sequence (from …ExpressionBacterialMutationBetaGal has two cysteine mutations (C77A, C1022S)…PromoterT7Available SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTrc99A-Rat FABP1
Plasmid#13577DepositorInsertRat liver fatty acid binding protein (Fabp1 Rat)
UseTagsExpressionBacterialMutationPromoterAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
Rtn3LΔ6LIR-mNeonGreen
Plasmid#169036PurposeExpress human Rtn3L with 6x LC3-interacting regions mutated in mammalian cellsDepositorInsertRtn3L (RTN3 Human)
UseTagsmNeonGreenExpressionMammalianMutationChanged following LC3-interacting regions of Rtn3…PromoterCMVAvailable SinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Human NANOS3-P2A-mCherry knock-in targeting construct
Plasmid#59721PurposeGene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised.DepositorInsertP2A mCherry
UseTagsExpressionMammalianMutationPromoterAvailable SinceDec. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
145-pGL3ctrl-3' UTR
Plasmid#28175DepositorInsertLuciferase flanked by tp53 UTRs (TP53 Human)
UseLuciferaseTagsExpressionMammalianMutationNote that insert length is based on sum of the 2 …PromoterAvailable SinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
GBSGFP insulator
Plasmid#121975PurposeGli activity reporter, mouse transgenesisDepositorInsertGBS(long)-GFP-insulator (Gnb5 Mouse)
UseMouse transgenesisTagsExpressionMutationPromoterAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-dSaCas9-2xNLS
Plasmid#107319PurposeExpresses SpCas9 fused to dSaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 is fused to nuclease dead SaCas9 (D10A, N5…PromoterCMV IE94Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSEMS-SDHB-Halo7Tag
Plasmid#108973PurposeSelflabeling tag at succinate dehydrogenase complex subunit B (SDHB)DepositorInsertsuccinate dehydrogenase complex iron sulfur subunit B (SDHB Human)
UseTagsHalo7-tagExpressionMammalianMutationPromoterCMVAvailable SinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPV-H1-ccdB-mEF1α-RiH
Plasmid#100598PurposepiggyBac vector for sgRNA cloningDepositorTypeEmpty backboneUsePiggybac vectorTagsExpressionMammalianMutationPromoterHuman H1 PolIII promoter, human FTH1 promoter, mo…Available SinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-hArfaptin1
Plasmid#206404PurposeMammalian expression of hArfaptin1DepositorAvailable SinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMDJ112 - pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]
Plasmid#159811PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUB-nHyPer2 (NLS:HyPer2:NLS)
Plasmid#84730PurposeExpresses HyPer2 in plant cells-biosensor for Hydrogen peroxide targeted to NucleiDepositorInsertHyPer2 targeted to nucleus
UseTagsNLS (nuclear localisation signal and NLS (nuclear…ExpressionPlantMutationPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable SinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
LP453
Plasmid#90184PurposepFLAG-CMV2 with cDNA encoding HsNPHP4 aa residues 1-839DepositorAvailable SinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSico-shRaptor
Plasmid#81086Purposeconditional expression of an shRNA targeting murine RaptorDepositorInsertRaptor shRNA (Rptor Mouse)
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6Available SinceSept. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10RFP-Pct5.1-crRNA(AarI)-RFP
Plasmid#192273PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(AarI)-RFP, entry plasmid for crRNA cloningDepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUB-nSypHer (NLS:sypHer:NLS)
Plasmid#84731PurposeExpresses sypHer in plant cells-biosensor for pH targeted to NucleiDepositorInsertnSypHer
UseTagsNLS (nuclear localisation signal and NLS (nuclear…ExpressionPlantMutationPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable SinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.9RT2-Pct5.1-crRNA(cadR)-RT(ΔcadR)
Plasmid#191647PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cadR-targeting spacer) cadR-deleting repair template,used for cadR deletionDepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2XNLS-SpCas9WT-NLS-3xHA-2XNLS-NmCas9WT-2XNLS
Plasmid#107324PurposeExpresses SpCas9 fused to NmCas9 in mammalian cellsDepositorInsertSpCas9-NmCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 is fused to NmCas9PromoterCMV IE94Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.1RT1-Pct5.1-crRNA(dadR)-RT(ΔdadR)
Plasmid#191635PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer) dadR-deleting repair template, used for dadR deletionDepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10-VEGFprom(-950 to -700)
Plasmid#66133PurposeLuciferase-based reporter for VEGF promoter region (-950 to -700 bp)DepositorInsertVascular Endothelial Growth Factor-A promoter region (VEGFA Human)
UseLuciferaseTagsExpressionMutationPromoterVEGF -950 to -700Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
HOXA10 (human) HIS-tag pET
Plasmid#8516DepositorAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-mouse Dicer
Plasmid#50357PurposeMouse Dicer cDNA cloneDepositorAvailable SinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
ATF4 7: 5’3’ATF4.GFP
Plasmid#21854DepositorInsertATF4 (Atf4 Mouse)
UseTagsEGFPExpressionMammalianMutationVector derived viral termination and poly-adenyla…PromoterAvailable SinceSept. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xMS2 oligo-library based
Plasmid#158200PurposeCassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted from the oligo pool experiment.DepositorInsert10 binding sites of MS2 from oligo library experiment
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cs2+Etv4(pea3)
Plasmid#49093Purposeexpression of Etv4DepositorInsertEtv4 (etv4 Zebrafish)
UseXenopus/avian/zebrafishTagsExpressionMammalianMutationPromoterCMVAvailable SinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
NET30 pEGFP-N2 (815)
Plasmid#62011Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEBG-MKK4 (GST)
Plasmid#21563DepositorInsertmitogen-activated protein kinase kinase 4 (Map2k4 Mouse)
UseTagsGSTExpressionMammalianMutationPromoterAvailable SinceJuly 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfGCaMP6s-T78H
Plasmid#100023PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutationDepositorInsertsfGCaMP6s-T78H
UseTags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFP and T78H m…PromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
NET55 pEGFP-N2 (610)
Plasmid#61992Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-ORF24-NTD
Plasmid#138464PurposeExpresses GST-tagged ORF24-NTD in bacteriaDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsGSTExpressionBacterialMutationAmino acids 1-201PromotertacAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-V5-SBP-HsSmg6-del36-576-siRNAres_I
Plasmid#146553PurposeMammalian Expression of HsSmg6-del36-576-siRNAresDepositorAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAT9750-BEAR-mCherry
Plasmid#162990PurposeBEAR target plasmid with split mCherry and disrupted 5' splice siteDepositorInsertmCherry split with an intron between amino acids 119-120
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-THAP11-rescue
Plasmid#37001DepositorInsertTHAP11 (THAP11 Human)
UseTags3xFLAGExpressionMammalianMutationMutations were generated at codons 273-275 conver…PromoterAvailable SinceMay 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pISH-Gnas
Plasmid#105483Purposeto generate a riboprobe for in situ hybridazationDepositorAvailable SinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only